Substrati enzimatici

<p>Luciferin (also in its salt form) is the substrate of luciferase, an enzyme that catalyzes a light-producing reaction. It is a bioluminescent substrate used to assay ATP availability. It also serves as a versatile scaffold to design other luminogenic enzyme substrates.</p>

<p>Chemiluminescent substrate for C8-esterase and lipase</p>

<p>Coelenterazine and its derivatives spontaneously complex with aequorin to develop a Ca2+-dependent fluorescent signal.</p>

<p>Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Fluorescein-dipalmitoylphosphatidylethanolamine triethylammonium salt is a fluorescent phospholipid derivative for phospholipase. This substrate consists of a fluorescein conjugated to DPPE (dipalmitoylphosphatidylethanolamine), a key component of biological membranes. This compound is frequently used in membrane dynamics studies and lipid metabolism research. The fluorescein moiety allows visualization of lipid interactions in model membranes, liposomes, or live-cell imaging applications. The triethylammonium salt form enhances solubility and stability, property that facilitates its use in aqueous and lipid-based environments.</p>

<p>Hippurate hydrolase is an enzyme that catalyzes the hydrolysis of hippurate to glycine and taurine. It is a fluorogenic substrate with high purity, ligand, and high quality. Hippurate hydrolase is used as a diagnostic enzyme for detecting the presence of hippuric acid in urine. This enzyme can be conjugated to a variety of molecules, including fluorophores, biotin or haptens, for use in immunoassay or other applications.</p>

<p>4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside is a chromogenic substrate for chitinase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside is used in different applications such as the Fungal infection detection, chitin degradation assays</p>

<p>6-Bromo-2-naphthyl b-D-glucopyranoside is an enzyme substrate used in chromogenic assays with beta-glucosidases. It has been used in culture media for the identification of Legionella pneumophila.</p>

<p>Aldol® 484 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by bacteria producing beta-glucosidase in liquid or solid media, yielding intense red color and green fluorescent signal. Aldol® 484 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Water soluble, chromogenic substrate for visualizing alkaline phosphatase activity. Produces a blueinsoluble end product that is detected visually when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situhybridization, ELISAs and immunohistochemistry.</p>

<p>Substrate for horseradish peroxidase</p>

<p>5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.</p>

<p>Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.</p>

<p>6-Chloro-3-indolyl butyrate is a chromogenic substrate for lipases. Use in lipid digestion and metabolism studies, it produces a salmon-pink color when 6-chloro-3-indolyl is released after enzymatic cleavage.</p>

<p>A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).</p>

<p>Also known as BCIP; can be used for visualization of phosphatase labelled antibodies on Western blots. This method quickly became extremely popular in diagnostics and biology research.</p>

<p>2-Naphthyl b-D-mannopyranoside is a chromogenic substrate specifically designed for the detection and quantification of β-mannosidase enzyme activity. Upon hydrolysis by β-mannosidase, this substrate releases a colored product, 2-naphthol, which can be easily monitored spectrophotometrically. The use of 2-Naphthyl b-D-mannopyranoside allows for the rapid and sensitive measurement of β-mannosidase activity in various applications, such as enzyme assays, screening for β-mannosidase-producing microorganisms, and studying the kinetics and inhibition of β-mannosidase enzymes.</p>

<p>Ac-DEVD-6-amino-D-luciferin is a substrate for luciferase that can be used in diagnostic, bioluminescence, and chemiluminescence assays. Ac-DEVD-6-amino-D-luciferin is a high quality compound that has been conjugated with various ligands. The CAS number for Ac-DEVD-6-amino-D-luciferin is 612061–87–8.</p>

<p>Chromogenic substrate for aryl-sulfatase. Yields a blue precipitate upon cleavage.</p>