Substrati enzimatici

<p>5-Bromo-3-indoxyl caprylate is a fluorogenic substrate that can be used for the detection of β-galactosidase. It is an enzyme substrate that can be used to measure β-galactosidase activity in cell culture media. It has a high purity and high quality. 5-Bromo-3-indoxyl caprylate is also used as a chromogenic substrate for the detection of β-galactosidase. This product has a CAS number of 133950-69-3.</p>

<p>Ac-DEVD-6-amino-D-luciferin is a bioluminescence substrate for protease enzymes (eg caspase-3 protease).</p>

<p>AquaSpark® H2S probe is a chemiluminescent probe for the detection of hydrogen sulfide (H2S), with excellent selectivity for sulfide over thiols. The AquaSpark® H2S probe is ~760-fold more selective for Na2S than to L-cysteine, and ~380-fold more selective for Na2S than for glutathione. AquaSpark® H2S probe was used in a study identifying antibiotic-resistant bacterial strains by detecting the beta-lactamase-driven hydrolysis of beta-lactam antibiotics, where hydrogen sulfide is a biodegradation metabolite (Popat Gholap et al, 2021).</p>

<p>Please enquire for more information about 3-Indoxyl choline phosphate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>AquaSpark® 510 acetate is a dioxetane-based luminogenic substrate for C2-esterase (acetyl esterase) activity. Upon hydrolytic cleavage by the enzyme AquaSpark® 510 acetate spontaneously emits green light. Esterases occur in all domains of life and fulfill important tasks in energy metabolism and recycling of cellular components. In microbiology C2-esterase activity can be used to detect specific microorganisms such as Bacillus cereus, Pseudomonas aeruginosa, Campylobacter jejuni and Candida albicans.</p>

<p>X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing β-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.<br>Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside (X-beta-D-galactoside, X-Gal) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate.<br>X-Gal can also be used as a detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the &amp;alpha;-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta-galactosidase, color develops within minutes to a few hours.</p>

AquaSpark™ β-D-galactoside is a dioxetane-based luminogenic substrate for β-galactosidase activity. β-galactosidase assays are frequently used in molecular biology as reporter gene assays. AquaSpark™beta-D-galactoside can be used to replace X-gal (Cymit Quimica Cat. No.B-7150) that is frequently used in genetically modified bacteria, in the yeast two-hybrid system, or in mouse models to indicate lacZ gene activity. Furthermore, native β-galactosidase activity can be used to detect coliform bacteria, which is important in microbial diagnostics and hygiene monitoring.

<p>AquaSpark® Calibration Probe is a water-soluble dioxetane-based chemiluminescent probe. Deprotonation of the free phenol group of the calibration probe under mild basic conditions results in an emissive decomposition process. No addition of enhancer or sensitizing agent is required to produce the bright green light emission in aqueous solutions.<br>AquaSpark® Calibration Probe is used for:</p>

<p>5-Bromo-4-chloro-3-indoxyl choline phosphate is a synthetic enzyme substrate for detecting phosphatidylcholine-specific phospholipase C.</p>

Chromogenic substrate for α-mannosidase yielding a salmon colored precipitate upon cleavage.

<p>5-Bromo-6-chloro-3-indoxyl phosphate, disodium salt monohydrate is a substrate used to visualize phosphatase activity via showing magenta colored end product when used with Nitroblue Tetrazolium.</p>

AquaSpark is a high-quality chemiluminescent substrate for the detection of bacterial, yeast, and fungal cells. It is a fluorogenic substrate that is used in diagnostic applications such as bacterial identification. The product has been tested for quality and purity, and it is suitable for use in culture media, food testing, and enzyme substrates. AquaSpark can be used in conjunction with a wide range of ligands to produce chemiluminescence. AquaSpark has been shown to have a superior performance when compared with other fluorogenic substrates used in the detection of bacteria. AquaSpark® alpha-L-idopyranosiduronic acid is an organic compound that belongs to the group of sugars. This product was designed by Ramot at Tel-Aviv University Ltd., Israel Patent family WO 2017/130191. It can be used as an analytical reagent to detect organisms such as bacteria, yeast, and fungi

<p>Substrate for horseradish peroxidase (HRP). HRP catalyzes oxidation of 4-chloro-1-naphthol in the presence of peroxide to the blue insoluble product 4-chloro-1-naphthon. It is used for both blotting and immunohistochemical staining but is less sensitive than other precipitating HRP substrates.</p>

<p>This product is a chromogenic substrate that is used in the detection of alkaline phosphatase and other enzymes. It is also used in food testing, staining, and diagnostics. This product has high purity with a CAS number of 125328-84-9.</p>

<p>AquaSpark® beta-D-apiofuranoside is a chemiluminescent substrate that can be used in the detection of various proteins, such as immunoglobulins, enzymes and hormones. The detection of these proteins can be done by the use of AquaSpark® beta-D-apiofuranoside conjugated with antibodies or enzymes. AquaSpark® beta-D-apiofuranoside is a substrate for the luciferase enzyme and it produces light when activated by a luciferin. This product has been tested for purity, quality and activity in various culture media. The product is also free from any detectable impurities and has passed all environmental testing requirements.</p>

<p>Chromogenic substrate for carboxylesterase yielding a blue precipitate upon cleavage. It has been proposed for the rapid detection of Branhamella catarrhalis in a strip test because unlike most other members of the family Neisseriaceae, Branhamella catarrhalis produces a butyrateesterase.</p>

<p>Aldol® 355 fluorescence enhancer is an enhancer of fluorescence signals of the Aldol® fluorogenic substrates. Aldol® 355 fluorescence enhancer allows concentrations of fluorogenic Aldol® substrates in media to reduce up to 6 times. Aldol® 355 fluorescence enhancer can be combined with Aldol® substrates, releasing water-insoluble dyes upon enzymatic cleavage, such as Aldol® 470 range, Aldol® 495 range, Aldol® 518 range, and Aldol®515 range of fluorogenic enzyme substrates.</p>

<p>Aldol® 495 beta-D-glucuronic acid is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucuronidase activity. The living cells of Escherichia coli can be reliably detected in growth media containing Aldol® 495 beta-D-glucuronic acid, as beta-glucuronidase occurs almost exclusively in E. coli. Aldol® 495 beta-D-glucuronic acid can also be in GUS reporter assays. Aldol® 495 beta-D-glucuronic acid can be used in liquid culture and solid media, and is suitable for use under aerobic and anaerobic conditions. Upon exposure to active beta-glucuronidase, Aldol® 495 beta-D-glucuronic acid develops orange colour and green fluorescence.</p>

<p>AquaSpark® H2S/-SH is a chemiluminescent probe for the detection of sulfides and thiols. The chemiluminescence obtained through sulfide is 3-5 fold higher than chemiluminescence obtained through thiols. AquaSpark® H2S/-SH probe was used in a study identifying antibiotic-resistant bacterial strains by detecting the beta-lactamase-driven hydrolysis of beta-lactam antibiotics, where hydrogen sulfide is a biodegradation metabolite (Popat Gholap et al, 2021).</p>

<p>AquaSpark® Broad Range Phosphatase Substrate is a chemiluminescent substrate for a variety of phosphatase enzymes including bacterial phosphatases and (calf intenstine) alkaline phosphatase (AP) commonly used in immuno-assays.</p>