Substrati enzimatici

<p>Chromogenic substrate targeting beta-galactosidase</p>

<p>Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate targeting alpha-L-arabinofuranosidase</p>

<p>2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is a chromogenic substrate for N-acetylglucosamine-6-sulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is used in different applications such as the Mucopolysaccharidosis IIID (Sanfilippo D) screening</p>

<p>5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-rhamnosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is used in bacterial rhamnose metabolism studies (e.g. E. coli).</p>

<p>Resorufin-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used in diagnostic applications. It is a conjugate of resorufin and beta-D-glucuronic acid. The enzyme substrate reacts with the enzyme luciferase to produce light. The high purity and quality of this product make it ideal for use in the detection of the presence of glucose in urine, which can be used to diagnose diabetes mellitus.</p>

<p>Chromogenic α-maltosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>Chromogenic substrate for beta-glucosidase</p>

<p>2-chloro-4-nitrophenyl-beta-D-cellotetraoside is a chromogenic substrate for measuring cellulase activity and other glycosyl hydrolases. The release of 2-chloro-4-nitrophenol can be detected at 405 nm.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that can be used in the detection of various enzymes. This product has been tested for sterility and purity, and it has passed all tests. It is also free of any detectable levels of heavy metals or other contaminants. 4NP2AG6S is a high quality bioluminescent substrate with CAS No. 71498-92-5. It is a fluorescent compound that reacts with an enzyme to produce light. The reaction can be detected by measuring the emitted light intensity at 680 nm (max).</p>

<p>N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a high quality and highly purified substrate for enzymes that catalyze the release of light when they react with their specific substrates. It is used in a variety of applications, including diagnostics and food testing. NACBZLAMC hydrochloride is an excellent ligand for chemiluminescence reactions and can be used to detect the presence of various analytes. It can also be used as a staining agent to detect proteins on Western blots or to stain bacterial cultures for identification. This product has been shown to be effective against numerous bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa, as well as fungi like Candida albicans.</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-D-galactofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is used in assays for detecting bacterial pathogens (e.g., Mycobacterium tuberculosis).</p>

<p>Substrate for firefly luciferase used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.</p>

<p>2-Nitrophenyl butyrate is a chromogenic enzyme substrate that is commonly used in enzymatic assays to detect lipases and esterases. When hydrolyzed by these enzymes, the substrate yields a yellow product that can be detected spectrophotometrically at 405 nm. The advantage of using 2-nitrophenyl butyrate is its high sensitivity and specificity for detecting lipolytic and esterolytic activities. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of esterase activity in biological samples such as serum, plasma, and cell lysates.</p>

<p>L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.</p>

<p>6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.</p>

<p>Hippurate hydrolase is an enzyme that catalyzes the hydrolysis of hippurate to glycine and taurine. It is a fluorogenic substrate with high purity, ligand, and high quality. Hippurate hydrolase is used as a diagnostic enzyme for detecting the presence of hippuric acid in urine. This enzyme can be conjugated to a variety of molecules, including fluorophores, biotin or haptens, for use in immunoassay or other applications.</p>

<p>Water soluble substrate for firefly luciferase. Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring. Endotoxin free product.</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.</p>

<p>Fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Used for the identification of Salmonella spp. based on the detection of caprylate esterase.</p>

<p>Fluorogenic substrate for carboxylesterase</p>

<p>L-Histidine 7-amido-4-methylcoumarin hydrochloride is a fluorogenic substrate, which is a synthetic chemical that is used as a substitute for natural substrates. It reacts with enzymes in the presence of hydrogen peroxide to produce light. This product has been used as an enzyme substrate, a diagnostic tool and has been used to detect bacterial growth in culture media. L-Histidine 7-amido-4-methylcoumarin hydrochloride is also used for food testing and environmental testing, where it can be used as a chromogenic substrate or conjugate respectively. This product has CAS number 5981-00-6 and molecular weight of 254.27 g/mol.</p>

<p>5-Bromo-4-chloro-1-methyl-3-indolyl-β-D-glucopyranoside is a fluorescent substrate used for the detection of β-glucuronidase. It can be used in several enzyme substrates, such as 5,5'-dithiobis(2-nitrobenzoic acid), 5,5'-dithiobis(3,4,5,-trimethoxybenzoic acid), and 4-(p-[N-(7-(2,7'-dimethyl -2H -chromenyl)-2H -indazolium)]phenylazo)benzoic acid. This product is used for diagnostics and culture media as well as food testing. The product has an extremely high purity and a CAS number.</p>

<p>PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.</p>

<p>6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.</p>

<p>Fluorogenic substrate for peroxidase</p>

<p>4-Methylumbelliferyl propionate is a fluorogenic substrate for esterase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl propionateas a substrate for measuring the esterase activity is used for esterase deficiency screening assays.</p>

<p>5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.</p>

<p>Phosphatase substrate</p>

<p>4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester, also known as 4-bromo-4'-(N,N-dimethylamino)biphenyl, is a bioluminescent substrate. It is used for the detection of beta-glucuronidase activity in biological samples and for the detection of bacterial β-glucuronidase activity in culture media. Beta-glucuronidase catalyzes the hydrolysis of glucuronic acid by breaking down a conjugate molecule into two smaller molecules, which can be detected using bioluminescence. 4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is a chromogenic substrate that has been shown to be an excellent ligand in biomolecular research because it reacts with many different enzymes. This product is highly pure and</p>

<p>Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.</p>

<p>2-Nitrophenyl b-D-fucopyranoside is a chromogenic substrate used in various enzymatic assays to detect the presence of fucosidase enzymes. It consists of a nitrophenyl group attached to a fucose sugar molecule. When acted upon by enzymes such as fucosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry.</p>

<p>Fluorogenic substrate targeting beta-glucosidase</p>

<p>6-Chloro-3-indolyl b-D-glucuronide sodium salt is a chromogenic substrate for beta-glucuronidase, a reporter gene used to monitor gene expression by detecting the enzyme's activity. It yields a salmon colored precipitate upon cleavage.</p>

<p>A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).</p>

<p>Luc-Salmonella is a bioluminescent substrate for bacterial luciferase use to detect Salmonella.</p>

<p>4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt (M3A or MU-GlcNS) is a fluorogenic substrate of heparin sulfamidase, used in the diagnosis of Mucopolysaccharidosis III type A (MPS III A). MPS III A, also known as Sanfilippo syndrome type A, is caused by the deficiency of heparan N-sulfatase. Heparin sulfamidase in the assay desulfates MU-GlcNS to 4-methylumbelliferyl 2-amino-2-deoxy-α-D-glucopyranoside (MU-GlcNH2). To release fluorometrically measurable 4-methylumbelliferone (MU) and by-product glucosamine, MU-GlcNH2 is further hydrolysed by an enzyme such as yeast α-glucosidase during a second incubation.</p>

<p>4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.</p>

<p>Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color</p>

<p>Chromogenic substrate for fucosyltransferases</p>

<p>Chromogenic substrate for beta-glucanase</p>

<p>6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for N-acetyl-beta-galactosaminidase. It produces a salmon-pink color after enzymatic cleavage.</p>

<p>6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is a fluorogenic substrate that is used in diagnostic applications. It has been shown to be a useful ligand for the determination of enzyme activity, and it can also be used to detect bacterial culture media contaminants. 6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is used as an enzyme substrate in chemiluminescent assays, and with other reagents, it can be used for the detection of environmental contaminants. This product has a CAS number of 55426-02-8 and can be found on the website www.sigmaaldrich.com/catalog/product</p>

<p>4-Nitrophenyl decanoate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl decanoate is used in different applications such as the Pancreatic insufficiency diagnosis, lipid metabolism studies</p>

<p>4-Methylumbelliferyl nonanoate is a synthetic fluorogenic substrate for lipase. It is used to measure the activity of lipases in vitro. 4-Methylumbelliferyl nonanoate reacts with a lipid substrate, such as sodium taurocholate, to produce 4-methylumbelliferone and dodecanol. The fluorescent product can be detected by fluorimetric or spectrophotometric methods. This compound is activated by an enzyme preparation, such as proteolytic enzymes, and can be used to measure the activity of these enzymes in vitro.</p>

<p>4-Nitrophenyl β-D-ribofuranoside is a synthetic chromogenic molecule designed to profile phosphorolytic enzyme activity, specifically ribonucleoside-hydrolyzing enzymes. Upon hydrolysis by these enzymes, it releases a 4-nitrophenolic compound, which can be monitored spectrophotometrically at 405-410 nm as a quantitative indicator of enzyme activity. This substrate is suitable for studying ribonucleoside-hydrolyzing enzymes, such as ribo- and deoxyribonucleases, in various biological systems and, in turn, could help advance our understanding of nucleic acid metabolism, gene function, and regulatory processes.</p>

<p>Fluorogenic substrate for esterase yielding a blue fluorescent solution upon cleavage.</p>

<p>A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.</p>