Substrati enzimatici

<p>Used in the MTT assay to asses the viability of cells. The yellow MTT is reduced by the living cells to a water-insoluble purple formazan. In order to make a quantitative measurement the formazan is often dissolved by addition of a solubilization solution to yield a colored solution that can be measured in a spectrophotometer. The rate of color formation reflects the dynamics and intensity of the microbial metabolism.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-3,4,6-tri-O-acetyl-b-D-glucopyranosyl)-4,6-di-O-acetyl-2deoxy--aDgalactopyranoside is a substrate for enzymes such as beta galactosidase and alpha amylase. It can be used for the detection of these enzymes in biological samples, including culture media and body fluids. 4NPAG has also been used as a chromogenic substrate to measure the activity of NADH oxidase in neutrophils. 4NPAG is fluorescent in aqueous solution and can be used to detect bioluminescence reactions.</p>

<p>4-Nitrophenyl 4,6-O-isopropylidene-a-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of beta-galactosidase. This product is high purity, high quality and has a CAS number of 29781-31-5. It is an enzyme substrate with chromogenic and diagnostic properties that are used for conjugation to other molecules, as well as for culture media, chemiluminescence and food testing. 4NP4IPG is also used in bioluminescence assays to detect the presence of beta-galactosidase.</p>

<p>4-Nitrophenyl b-D-cellopentaoside hexadecaacetate is a fluorogenic substrate that is used to study the activity of various enzymes. It reacts with hydrolytic enzymes in the presence of hydrogen peroxide to produce light, and it can be used for food testing, environmental testing, and diagnostics. 4-Nitrophenyl b-D-cellopentaoside hexadecaacetate is a conjugate of 4-nitrophenol and cellobiose pentaacetate that has a high purity and high quality. The CAS number for this compound is 129411-66-1.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-3,4,6-tri-O-acetyl-2 deoxy -D-galactopyranosyl]-b D glucopyranoside is a fluorescent substrate for the enzyme β galactosidase. It can be used to test for β galactosidase activity in food products, environmental samples and diagnostics. 4NPFAPG is a conjugate of 4 nitrophenol and 2 acetamido 3 O-[2 O-(2,3,4 tri O benzoyl a L fucopyranosyl) 3,4 6 tri O acetyl 2 deoxy D galactopyranosyl] b D glucopyranose. 4NPFAPG is water soluble and has</p>

<p>N-Acetyl-L-glutamine p-nitroanilide is a fluorogenic substrate that can be used in the diagnosis of bacterial infections. It is a ligand that can bind to enzymes involved in the production of bioluminescence, such as luciferase, and can be used for the detection of bacteria. It is also used for food testing, environmental testing, and diagnostics. N-Acetyl-L-glutamine p-nitroanilide has high purity and high quality, which makes it suitable for conjugation with other molecules.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(a-L-fucopyranosyl)-b-D-glucopyranoside is a fluorescent substrate for beta-galactosidase that is used in diagnostics and research. The product has been shown to be an excellent substrate for beta-galactosidase and beta-glucuronidase, as well as other glycosidases. It can be used to detect the presence of these enzymes in any sample containing glycosides. This product is also used in food testing, environmental testing, and bioluminescence experiments. 4NPAFG is available in high purity with a conjugate group at the C4 position.</p>

<p>4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. It can be used in diagnostic tests to identify antibodies against certain bacteria and fungi. The fluorogenic form of 4-methylumbelliferyl 2-acetamido-3,4,6-triO acetyl -2 deoxy a D glucopyranoside can be used as a chemiluminescent substrate for detecting bacterial activity. 4 methylumbelliferyl 2 acetamido 3,4,6 triO acetyl -2 deoxy a D glucopyranoside is also useful in bioluminescent assays to detect ATP production in bacteria and other microorganisms. CAS No: 1174287–76–3</p>

<p>4-Nitrophenyl 2,3,4-tri-O-acetyl-a-D-glucopyranoside is a high quality chemical used in the development of various diagnostic and research applications. It is also used in the production of enzymatic substrates for enzyme activity assays. 4NPTAG is a chromogenic substrate that produces chemiluminescence when it reacts with an oxidizing agent and has been shown to be very effective as a fluorogenic substrate for protein kinases. This compound has been shown to bind to ligands and can be used as a bioluminescent reporter for molecular biology applications. The CAS number for this product is 54973-14-0.</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3-O-(2,3,4,6-tetra -O acetyl -b D galactopyranosyl)-2 deoxy a D galactopyranoside is a high purity fluorogenic substrate for the enzyme β galactosidase. It can be used for the detection of β galactosidase in biological samples. 4NPAG can also be used as a ligand for various types of chromogenic substrates. This product is also commonly used in diagnostics and conjugates to detect β galactosidase activity or as a culture media supplement.</p>

<p>4-Nitrophenyl 2,3,4-tri-O-acetyl-6-O-trityl-a-D-glucopyranoside is a substrate for chemiluminescent assays. It is a high quality chemical that can be used in environmental testing, staining, ligand binding and bioluminescence. 4NP2T6AODG is used as a chromogenic substrate or fluorogenic substrate to detect the presence of enzymes in diagnostics and food testing. This product has CAS No. 67794-14-0 and can be purchased at high purity.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-3,6-di-O-benzoyl-b-D-galactopyranoside is an advanced pNP-based enzyme substrate designed for the detection and measurement of glycosidase activity in biological samples. This synthetic compound offers high specificity and sensitivity for b-galactosidase enzymes, enabling accurate determination of enzymatic hydrolysis rates in a variety of research applications, including diagnostics, drug development, and discovery of novel enzymes. Its chromogenic properties ensure easy analysis of the results and make it suitable for high-throughput screening.</p>

<p>Aldol® 455 is a substrate for the enzyme beta-galactosidase. It is used in food testing, staining, and environmental testing. Aldol® 455 has been shown to be a good substrate for fluorogenic and chromogenic substrates. It can be conjugated with an antibody or other ligand for use in immunoassays. Aldol® 455 is of high purity and high quality, making it suitable for use in diagnostics and as an enzyme substrate. This product can also be used as a culture media additive.</p>

<p>2-Chloro-4-nitrophenyl b-D-galactopyranoside is a chromogenic enzyme substrate used to study the activity of beta-lactoside. It produces a yellow color when cleaved by the enzyme, making it a useful tool in enzyme assays and screening experiments.</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-mannopyranoside is a highly sensitive and efficient chromogenic substrate designed for the detection and quantification of α-D-mannosidases. These enzymes play a crucial role in the degradation and metabolism of glycoproteins and glycolipids, thus having high importance in biotechnology and biochemical research. When acted upon by α-D-mannosidase, this substrate releases a bright yellow product, 4-nitrophenol, which is easily measured spectrophotometrically at 405 nm. This enables quick and accurate analysis in various applications such as enzyme kinetics, inhibitor screening, and activity assays.</p>

<p>S-(4-Nitrophenyl)mercapturic acid (S-NPMA) is the major metabolite of 4-nitrophenol, which is a precursor to dyes and pharmaceuticals. S-NPMA has been detected in urine samples from humans, rats, and mice. The main metabolic pathway for S-NPMA is an oxidative conversion by cytochrome P450 enzymes. S-NPMA has been found to be a useful biomarker for the presence of polycyclic aromatic hydrocarbons in wastewater and human urine. The levels of this compound have also been shown to increase with age in humans.</p>

<p>4-Nitrophenyl 2,3-di-O-(b-D-glucopyranosyl)-b-D-glucopyranoside is a chromogenic substrate that can be used to detect the presence of β-glucuronidase in human and animal tissue. The compound is also a fluoroquinolone substrate for the detection of β-lactamase activity. 4NPBG is used in diagnostics as well as food testing, due to its ability to detect bacteria in both food and water samples. 4NPBG is a chemiluminescent substrate that emits light upon hydrolysis by β-glucuronidase or β-lactamase. The compound has been shown to be an excellent ligand for binding to protein A or protein G and can be used for immunoassays such as ELISA or Western blotting.</p>

<p>2-Bromo-4-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that is used for the detection of β-galactosidase activity in the biochemical reaction. It has been shown to be an excellent ligand for metal ions and can be used as a reagent in the determination of metal ions in biological systems. This product is manufactured with high purity and quality, which ensures its suitability for use in culture media, environmental testing, and diagnostics. 2-Bromo-4-nitrophenyl b-D-galactopyranoside has been tested as a fluorogenic substrate for β-galactosidase. It has also been used as a chromogenic substrate for β-galactosidase and an enzyme substrate for β-galactosidase. This product can be conjugated with other molecules such as biotin to enable rapid analysis of proteins or</p>

<p>6-Bromo-2-naphthyl-alpha-D-mannopyranoside is a strong inhibitor of alpha-mannosidase. It has been shown to inhibit the growth of Mycobacterium tuberculosis and Streptococcus pneumoniae, which are both known to cause bacterial infections in humans. 6BNA inhibits the enzyme alpha-mannosidase, which hydrolyzes terminal α-(1,4)-linked mannose residues from oligosaccharides and glycoproteins. This drug has a high affinity for the active site of alpha-mannosidase and an IC50 value of 0.2 mM, making it an effective inhibitor.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-acetyl-4,6-O-benzylidene-2-deoxy-aDglucopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity. It is a water soluble substrate with an extinction coefficient of approximately 10,000 M−1 cm−1 at 420 nm. 4NPAABGDG is a chromogenic substrate that can be used to detect β-galactosidase activity. It has an extinction coefficient of approximately 1,700 M−1 cm−1 at 420 nm. The CAS number for 4NPAABGDG is 23262–56–8 and it is sold by Sigma Aldrich under catalog number L4959.</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside is a versatile chromogenic substrate designed for the detection and quantification of various glycosidase enzymes. This substrate is widely used in enzyme assays, producing a yellow color upon cleavage by target enzymes. The 4-Nitrophenol released in this reaction can be easily monitored spectrophotometrically, allowing for rapid and accurate enzyme analysis. This high-quality substrate provides a convenient and reliable tool for researchers in biochemistry, enzymology, and related fields.</p>

<p>4-Nitrophenyl 4-O-(b-D-mannopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity in biological samples. It is an excellent substrate for the enzyme β-glucosidase, which catalyzes the hydrolysis of ester bonds in glycosides with release of glucose and formation of a phenolic product. The 4-nitrophenol group reacts with oxygen to produce light, making it useful as a chemiluminescent or bioluminescent substrate. The conjugate may also be used to detect β-glucosidase activity in culture media by adding the substrate to the culture medium and measuring its fluorescence.<br>4NP4OBG has been shown to have high purity, with only trace amounts of impurities detected such as benzaldehyde and 2,6-dinitro</p>

<p>4-Nitrophenyl b-D-cellohexaoside is a substrate for many enzymes and can be used to detect the presence of these enzymes in biological samples. It can also be used to detect the presence of bacteria, fungi, parasites, and viruses. 4-Nitrophenyl b-D-cellohexaoside is an excellent substrate for enzyme reactions that produce a chromogenic or fluorogenic product. This product is often conjugated with other compounds such as antibodies or antigens to form an immunoassay. 4-Nitrophenyl b-D-cellohexaoside is made from a high purity chemical by a process called nitration with sodium nitrate and sulfuric acid.</p>

<p>Z-Gly-Pro-Arg-pnaacetate is a substrate commonly used in enzyme research, particularly in the study of proteases. Proteases are enzymes responsible for catalyzing the hydrolysis of peptide bonds in proteins. In a study investigating the activation of protein C in blood plasma by a protease from Aspergillus ochraceus, the enzyme activity was qualitatively assessed using chromogenic peptide substrates, such as pGlu-Pro-Arg-pNA.</p>

<p>L-Citrulline-p-nitroanilide hydrobromide is a substrate for enzymes such as beta-galactosidase, beta-glucuronidase, and alkaline phosphatase. It is used in biochemical assays for the detection of microorganisms and in medical diagnostics. This compound has been shown to be useful in fluorescence microscopy and bioluminescence. It also has applications in food testing, environmental testing, and high purity research.</p>

<p>6-Benzoyl-2-naphthylphosphate disodium salt is a chromogenic substrate used to detect and quantify alkaline phosphatase activity. After enzymatic hydrolysis, 6-benzoyl-2-naphthol is released, which can be detect or even further react with other reagents (e.g. Fast Red) to increase the intensity of the colorimetric signal. It is used in common applications such as ELISA, immunohistochemistry and Western Blotting.</p>

<p>For asymmetric double bond reduction.</p>

<p>4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity</p>

<p>Fluorogenic substrate targeting phosphatase</p>

<p>1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.</p>

<p>Used in biological studies as a chromogenic substrate for β-galactosidase; red color.Chlorophenol red indicates changes of pH in the range of 4.8 to 6.7 by turning the solution from yellow (at pH 4.8) to violet (at pH 6.7). Converted as signalogenic moiety of an enzyme substrate, chlorophenol red beta-D-galactopyranoside has a good proven sensitivity in the detection of beta-galactosidase activity in transfected mammalian cells, when compared to ONPG (o-nitrophenyl-beta-D-galactopyranoside) (Eustice, 1991)</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(β-D-galactopyranosyl)-α-D-glucopyranoside is a high-quality chromogenic pNP enzyme substrate specifically designed to enable effective and accurate detection of enzyme activity. By utilizing this advanced substrate, researchers and professionals can perform a range of applications, including activity assays, enzyme inhibition studies, and substrate specificity profiling. This substrate's unique properties result in exceptional sensitivity and throughput, ensuring effective results for a wide variety of enzyme assays.</p>

<p>Substrates for endoglucanase and beta-cellobiohydrolase activity in the study of digestive system in Zygentoma as an insect model for high cellulase activity</p>

<p>2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside is a chromogenic substrate used for beta-N-acetylglucosaminidase assays. Upon enzymatic hydrolysis, 2-chloro-4-nitrophenol is released, which has a yellowish color. It is used in diagnostic applications such as lysosomal storage disorder diagnosis (for example, Tay-Sachs disease) and glycosidase inhibition studies.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>1,8,15,22-Tetrazacyclooctacosane-2,7,16,21-tetraone is an inhibitor with potent anticancer properties. It induces apoptosis in cancer cells by inhibiting kinases that are critical for cell survival and proliferation. This compound has been shown to be effective against various types of cancer in both Chinese hamster ovary and human cancer cell lines. Additionally, it has been found to increase the excretion of the anti-allergic drug rupatadine in urine. The use of 1,8,15,22-Tetrazacyclooctacosane-2,7,16,21-tetraone as a tumor inhibitor has also been investigated in combination with other kinase inhibitors such as bendamustine analogs. This compound holds great potential for the development of new anticancer therapies.</p>

<p>4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1-&gt;3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.</p>

<p>3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Used for synthesis chiral amines from ketones</p>

<p>4-Aminoantipyrine has been used as an analgesic and anti-inflammatory agent. It can also target peroxidase enzymes to produce a red color</p>

<p>Used for synthesis of chiral amines from ketones and ketoacids</p>

<p>Used for synthesis of chiral carboxylic acids from nitriles</p>

<p>Fast Blue BB is commonly used for assaying beta-glycosidase activity and the chromogenic reaction between Fast Blue BB and beta-naphthol results in a reddish colored product and is at the basis of its application to different enzymatic activity assays. Produces a fluorescent, blue-green chromophore.</p>

<p>COX inhibitor; nonsteroidal anti-inflammatory drug</p>

<p>2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-a)pyrazin-3-one hydrochloride (ABI) is a potent and selective inhibitor of the enzyme toll-like receptor. The drug has been shown to decrease the metabolic rate in experimental models. ABI has also been used as an analog for other compounds to study their effects on redox potentials and reactive species. It is unclear how ABI inhibits protein synthesis by polymerase chain reaction (PCR). One possible mechanism is that it may inhibit the binding of primers to DNA. ABI also inhibits the production of chemokines which are small proteins that stimulate inflammatory responses. This drug can be used in autoimmune diseases such as rheumatoid arthritis and multiple sclerosis.</p>

<p>Remazol Brilliant Blue R D-xylan is a chromogenic substrate used for the detection and quantification of xylanase enzyme activity. This substrate consists of a xylan polymer backbone covalently linked to the Remazol Brilliant Blue R dye. Upon cleavage by xylanase enzymes, the dye is released, producing a vibrant blue color that can be easily measured spectrophotometrically. This substrate is widely used in various applications, including the study of xylanase-producing microorganisms, optimization of enzyme production, and evaluation of enzyme kinetics.</p>

<p>The AquaSpark® 510 Nitroreductase/NADH Probe (CL-NTR) is a water-soluble probe for the selective chemiluminescent detection of nitroreductase (NTR) activity. It is a versatile tool for monitoring a number of biological processes.</p>

<p>4-Nitrophenyl 2-azido-2-deoxy-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-azido-2-deoxy-beta-D-galactopyranoside is used in different applications such as the Bacterial enzyme assays (e.g., E. coli detection)</p>

<p>2,4-Dinitrophenyl)-L-lysine hydrochloride monohydrate is a lysine derivative that has been modified with a 2,4-dinitrophenyl group. It could be used as an enzyme substrate to monitor enzymes that recognize and modify lysine residues, such as lysine methyltransferases and demethylases, lysine acylases and lysine oxidases.</p>