Substrati enzimatici

(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.

Nalpha-CBZ-L-Citrulline-p-nitroanilide is a synthetic chromogenic enzyme substrate used in protease enzymatic stutides. After hydrolysis, p-nitroaniline is released, which produces a yellow color detectable spectrophotometrically at 405 nm.

4-Methylumbelliferyl palmitate, commonly names as 4-MUP, is a fluorescent probe that binds to lipases, enzymes responsible to assist on the hydrolysis of fats. In diagnosis, 4-MUP is used for screening of lysosomal storage diseases (LSD) caused by a deficiency of a single enzyme involved in the metabolism of glycoproteins, lipids or mucopolysaccharides.

Aldol® 470 acetate is a Fluorogenic substrate that is used in enzymatic reactions to produce a fluorescent product. Aldol® 470 acetate can be used as a ligand or a chromogenic substrate. This product is also used in diagnostic tests, such as conjugates and chemiluminescence, to detect the presence of certain enzymes or microorganisms. It can be used in culture media to measure the amount of certain enzymes, such as phosphatases or proteases, and has been shown to have high purity and quality. This product has CAS No. 1318785-37-3, 1318785-38-4, 1318785-39-5, 1318785-40-6 and 1318785-41-7.

2-Chloro-4-nitrophenyl a-D-maltotrioside (2CNP) is a potent hypoglycemic agent that has been shown to decrease postprandial blood glucose levels in humans. 2CNP is a white crystalline solid that is soluble in water and ethanol. The transfer reactions of 2CNP are enhanced by benzalkonium chloride, which forms an organic complex with the drug. The optimum concentration for the hypoglycemic effect of 2CNP is determined to be 10 μM, which can be detected using an optical sensor. This compound also inhibits α-amylase and other enzymes involved in carbohydrate metabolism, leading to the accumulation of glycogen and lowering the blood glucose level.

Chromogenic substrate for α-glucanase

5-Bromo-6-chloro-3-indolyl acetate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in bacterial esterase detection (e.g. Staphylococcus spp.).