Substrati enzimatici
I substrati enzimatici sono molecole su cui agiscono gli enzimi per catalizzare reazioni chimiche. Il substrato si lega al sito attivo dell'enzima, dove subisce una trasformazione specifica per formare il prodotto della reazione. I substrati enzimatici sono strumenti essenziali nella ricerca per studiare la cinetica enzimatica, la specificità e il meccanismo d'azione. Sono utilizzati anche in saggi diagnostici e studi biochimici. Presso CymitQuimica, offriamo una selezione diversificata di substrati enzimatici di alta qualità per supportare le vostre ricerche in enzimologia e biochimica.
Sottocategorie di "Substrati enzimatici"
Trovati 1527 prodotti di "Substrati enzimatici"
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AquaSpark® 510 Calibration Probe, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191
CAS:AquaSpark® Calibration Probe is a water-soluble dioxetane-based chemiluminescent probe. Deprotonation of the free phenol group of the calibration probe under mild basic conditions results in an emissive decomposition process. No addition of enhancer or sensitizing agent is required to produce the bright green light emission in aqueous solutions.
AquaSpark® Calibration Probe is used for:
Demonstration experiment susing only the probe and an aqueous solution or water;
Evaluation of the AquaSpark® maximum light emission;
Functional tests of luminometers, plate readers and cameras;
Comparison with other probes bearing an enzyme labile group in order to build concentration/response curves;
Validation and comparison between different AquaSpark® probes.Formula:C21H23ClO6Purezza:Min. 95%Colore e forma:PowderPeso molecolare:406.12 g/molIsopropyl-β-D-thiogalactopyranoside - non-animal origin
CAS:A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.Formula:C9H18O5SPurezza:Min. 99.0 Area-%Peso molecolare:238.3 g/mol2-Fluoro-4-nitrophenyl b-D-galactopyranoside
CAS:2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.Purezza:Min. 95%Colore e forma:PowderPeso molecolare:319.24 g/mol4-Methylumbelliferone
CAS:4-Methylumbelliferone is a coumarin derivative that inhibits the activity of enzymes such as p-nitrophenyl phosphate oxidase and aminopeptidase. It is used in vitro to study oxidative injury, which plays a role in the pathogenesis of inflammatory diseases, including primary sclerosing cholangitis and bone marrow transplantation. 4-Methylumbelliferone has been shown to induce apoptosis by increasing reactive oxygen species levels and activating toll-like receptor 4 (TLR4). It also has inhibitory effects on osteosarcoma cell proliferation in vitro.Formula:C10H8O3Peso molecolare:176.17 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-glucopyranoside-6-sulfate sodium salt
CAS:4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.Formula:C18H20NO11SNaPurezza:Min. 98 Area-%Colore e forma:White PowderPeso molecolare:481.41 g/mol4-Methylumbelliferyl b-D-cellopentoside
CAS:A fluorogenic MOSCERDAM® substrate targeting cellulasesFormula:C40H58O28Purezza:Min. 95%Colore e forma:White To Off-White SolidPeso molecolare:986.89 g/mol4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-β-D-glucopyranoside
4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.Formula:C24H26O11SPurezza:Min. 95%Colore e forma:White to off-white solid.Peso molecolare:522.52 g/molL-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt
CAS:L-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.Formula:C19H18N2O3•C2HF3O2Purezza:Min. 98 Area-%Colore e forma:White PowderPeso molecolare:436.38 g/mol4-Methylumbelliferyl lignocerate
CAS:This botanical is a download for the Floral Database.
Formula:C34H54O4Purezza:Min. 95 Area-%Peso molecolare:526.81 g/molRef: 3D-M-5720
1gPrezzo su richiesta5gPrezzo su richiesta250mgPrezzo su richiesta500mgPrezzo su richiesta2500mgPrezzo su richiesta-Unit-ggPrezzo su richiestaN-Acetyl-glycine p-nitroanilide
CAS:Substrate for hydrolasesFormula:C10H10N2O5Purezza:Min. 95%Colore e forma:Yellow powder.Peso molecolare:238.2 g/mol5-Bromo-3-indolyl-β-D-galactopyranoside-6-sulfate sodium salt
CAS:5-Bromo-3-indolyl-beta-D-galactopyranoside-6-sulfate sodium salt is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in sulfatase activity assays and for the diagnosis of Mucopolysaccharidosis IVA (Morquio A).Formula:C14H15BrNNaO9SPurezza:Min. 95%Colore e forma:PowderPeso molecolare:476.23 g/mol3-Nitrophenyl b-D-galactopyranoside
CAS:Chromogenic substrate targeting beta-galactosidaseFormula:C12H15NO8Colore e forma:PowderPeso molecolare:301.25 g/mol4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxy-b-D-galactopyranoside
CAS:4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.
Formula:C26H38N2O17Purezza:Min. 95%Peso molecolare:650.58 g/mol5-Bromo-6-chloro-3-indolyl α-D-glucopyranoside
CAS:5-Bromo-6-chloro-3-indolyl α-D-glucopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in Pompe disease diagnostics, a glycogen storage disorder, that measures the deffiency of alpha-glucosidase (GAA) that could lead to the accumulation of glycogen in lysosomes.
Formula:C14H15BrClNO6Purezza:Min. 95%Colore e forma:PowderPeso molecolare:408.63 g/mol6-Chloro-3-indolyl butyrate
CAS:6-Chloro-3-indolyl butyrate is a chromogenic substrate for lipases. Use in lipid digestion and metabolism studies, it produces a salmon-pink color when 6-chloro-3-indolyl is released after enzymatic cleavage.Formula:C12H12ClNO2Purezza:Min. 95%Colore e forma:PowderPeso molecolare:237.68 g/mol2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt
CAS:2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt is a chromogenic substrate commonly used in the detection and quantification of sialidase activity. Sialidases are a family of enzymes that hydrolyze sialic acid residues from glycoconjugates. This substrate is used to detect bacterial and viral sialidases, which play an important role in pathogen infectivity. The ammonium salt form of the substrate is preferred for its solubility in water-based assay systems.Purezza:Min. 95%Peso molecolare:447.39 g/molBCM® O157:H7(+)
BIOSYNTH Culture Medium for the isolation andidentification of Escherichia coli O157:H7
4-Nitrophenyl b-D-thiogalactopyranoside
CAS:4-Nitrophenyl b-D-thiogalactopyranoside is a specialized chromogenic substrate designed for measuring the activity of thio-β-galactosidase enzymes. Upon cleavage by thio-β-galactosidase, it releases the intensely colored 4-nitrophenolate ion, providing a straightforward, sensitive, and quantitative detection method. This substrate is easily incorporated into enzyme-coupled assays, making it a reliable and convenient choice for research and diagnostic applications. With its rapid color development and high signal-to-background ratio, 4-Nitrophenyl b-D-thiogalactopyranoside is ideal for monitoring enzyme kinetics, substrate specificity evaluation, and inhibitor screening in a range of scientific and biotechnological settings.Formula:C12H15NO7SPurezza:Min. 98 Area-%Colore e forma:PowderPeso molecolare:317.32 g/mol5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside
5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-D-galactofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is used in assays for detecting bacterial pathogens (e.g., Mycobacterium tuberculosis).Formula:C14H15BrClNO6Purezza:Min. 95%Colore e forma:PowderPeso molecolare:408.63 g/molL-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride
CAS:L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.Purezza:Min. 98.0 Area-%Colore e forma:Powder
