Substrati cromogenici

<p>5-Iodo-3-indolyl b-D-galactopyranoside is a chromogenic substrate used for beta-galactosidase. It is incorporated into media or detection kits for rapid and direct identification of microorganisms.</p>

<p>Aldol® 518 alpha-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of alpha-galactosidase activity. The enzyme substrate is cleaved by alpha-galactosidase positive bacteria and yeasts in liquid or solid media, yielding red color and red fluorescence signal. Aldol® 518 alpha-D-galactopyranoside can also be used to detect alpha-galactosidase (encoded by the Mel1 gene) in yeast gene reporter assays or for identification of alpha-gal positive transformants on agar plates. Aldol® 518 alpha-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate targeting cellulases</p>

<p>Aldol® 455 beta-D-galactopyranoside is a chromogenic substrate for the enzyme β-galactosidase. It is used to detect the activity of β-galactosidase in cell culture, and has been shown to be highly purified and of high quality. This product can be used as a ligand for receptor binding studies or as an agent in enzyme substrates in fluorescence and chemiluminescence reactions.</p>

<p>A substrate for visualising alkaline phosphatase activity. Produces a red colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.</p>

<p>Bronze-Gal (Bronze-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a bronze colored precipitate. Bronze-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>5-Cyano-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that can be used to measure the activity of esterases and other enzymes. It has been shown to be a potent inhibitor of human erythrocyte esterase. The compound is also a potent inducer of bioluminescence in bacteria, which can be used for diagnostic applications. 5-Cyano-3-indolyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of phosphatases and other enzymes. This product has an enzyme substrate function, which can be used for enzyme assays. 5CIGP is useful for measuring high levels of enzyme activity in the presence of strong inhibitors such as phenylmethylsulfonyl fluoride (PMSF).</p>

<p>4-Nitrophenyl-beta-D-glucopyranoside is a fluorescent probe that can be used in the study of the biochemical properties of enzymes. This compound binds to the enzyme's active site, which allows for transfer reactions to occur. 4-Nitrophenyl-beta-D-glucopyranoside is an artificial substrate that is biochemically inert and has a similar physical structure to natural substrates. It can be used as a model system to study enzymatic reactions and has been shown to have pharmacokinetic properties and a pH optimum. The detection sensitivity of this compound is high, making it ideal for use in biochemical studies.</p>

<p>2-Nitrophenyl a-D-glucopyranoside is a chromogenic substrate commonly used to assay the activity of glucosidases. Upon cleavage of the glycosidic bond by glucosidases, the released 2-nitrophenyl group yields a yellow color detectable by spectrophotometry. This substrate has high sensitivity and is widely used in a variety of applications, including drug discovery and enzyme kinetics studies.</p>

<p>Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of beta-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of beta-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>5-Bromo-4-chloro-3-indoxyl-a-D-xylopyranoside is a fluorogenic substrate that can be used in food testing and environmental testing. This product is a conjugate of the xylosidase enzyme (EC 3.2.1.37) with 5-bromo-4-chloroindoxyl, which has been shown to have a high affinity for the enzyme's active site and enzymatic activity. The purity of this product is &gt; 95% and the CAS number is 68618-41-5.</p>

<p>3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.</p>