Substrati cromogenici

N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.

Aldol® 470 acetate is a Fluorogenic substrate that is used in enzymatic reactions to produce a fluorescent product. Aldol® 470 acetate can be used as a ligand or a chromogenic substrate. This product is also used in diagnostic tests, such as conjugates and chemiluminescence, to detect the presence of certain enzymes or microorganisms. It can be used in culture media to measure the amount of certain enzymes, such as phosphatases or proteases, and has been shown to have high purity and quality. This product has CAS No. 1318785-37-3, 1318785-38-4, 1318785-39-5, 1318785-40-6 and 1318785-41-7.

8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.

Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Naphthol AS-OL is a widely used chromogenic enzyme substrate that forms a colored product upon reaction with specific enzymes. It is commonly employed in histochemical and cytochemical staining techniques to visualize enzyme activity in cells and tissues. Naphthol AS-OL is particularly useful for detecting the presence of enzymes such as alkaline phosphatase, acid phosphatase, and esterases. The resulting colored product can be easily observed under a microscope, allowing researchers to study the distribution and localization of these enzymes in various biological samples.

Chromogenic substrate used to measure phospholipase C (PLC) activity

A chromogenic substrate for β-galactosidase.

Chromogenic substrate for beta-glucosidase

Chromogenic substrate for β-D-Cellobiosidase yielding a yellow solution upon cleavage.

4-Nitrophenyl decanoate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl decanoate is used in different applications such as the Pancreatic insufficiency diagnosis, lipid metabolism studies

The 4-Nitrophenyl a-D-xylopyranoside is a highly sensitive and reliable chromogenic substrate tailored for the detection and analysis of alpha-D-xylosidase enzyme activity. Upon enzymatic hydrolysis, it forms 4-nitrophenol, a yellow compound with remarkable fluorescence that enables a straightforward, quantitative assay in areas including environmental science, metabolism studies, and enzyme modifier exploration.

2-Naphthyl beta-D-galactopyranoside is a glycoside that is found in the flowers of the plant Eschscholzia californica. It is used as a localizer for bacterial strains and can be hydrolyzed by enzymes to release naphthalene. 2-Naphthyl beta-D-galactopyranoside has been shown to inhibit the growth of corynebacterium and diazonium salt, which may be due to its ability to bind to DNA and disrupt protein synthesis. The potential use of this molecule is for its insolubility, which prevents it from being absorbed by skin cells.

Modified naphtol AS substrate used in histochemical and cytochemical staining

4-Nitrophenyl 3-O-(beta-D-glucopyranosyl)-alpha-D-glucopyranoside is a chromogenic substrate for alpha-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 3-O-(beta-D-glucopyranosyl)-alpha-D-glucopyranoside is used in different applications such as the Gaucher disease screening, carbohydrate metabolism studies

Calcium 2-naphthylphosphate, which is the calcim salt of 2-naphthylphosphate, is a chromogenic substrate to measure alkaline phosphatase activity. After enzymatic cleavage, the product yielded (2-naphthol) generates a red-colored complex upon reaction with a diazonium salt like Fast Blue RR or Fast Garnet GBC.

2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.

2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid, also known as p-nitrophenyl N-acetylneuraminic acid, is a chromogenic substrate specifically designed for the detection and quantification of neuraminidase (sialidase) activity. Upon cleavage by neuraminidase, it releases a yellow-colored product, 4-nitrophenol, which can be easily monitored spectrophotometrically at 405 nm. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of neuraminidase activity in biological samples such as viruses, bacteria, and cell lysates. It is particularly useful for studying the enzymatic properties of neuraminidases from different sources and for the development of novel antiviral and antibacterial agents targeting neuraminidase activity.

Aldol® 467 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 467 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 467 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

Aldol® 515 beta-D-xylopyranoside is a chemiluminescent ligand that can be used for the detection of beta-xylosidase activity to yield a red color.

Naphthol AS-D is a diagnostic chemical for phosphatase enzymes and is used to stain cells, tissues, and body fluids for diagnosis. The chemical reacts with the amino groups of proteins in cells and produces a brown color. Naphthol AS-D is not toxic to living cells in concentrations below 5%. It stains basophilic leukemia cells as well as brain cells. This chemical can be used to diagnose cutaneous lesions, such as psoriasis or melanoma, which are difficult to diagnose by standard methods.  present in these lesions and produce a brown color.

6-Chloro-3-indolyl b-D-glucuronide sodium salt is a chromogenic substrate for beta-glucuronidase, a reporter gene used to monitor gene expression by detecting the enzyme's activity. It yields a salmon colored precipitate upon cleavage.