Human NP-B(Neuropeptide B) ELISA Kit
Rif. EK-ELK0059
48T | 391,00 € | ||
96T | 560,00 € |
Informazioni sul prodotto
- L7; PPNPB; PPL7; NPB
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NP-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NP-B in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK0059 Human NP-B(Neuropeptide B) ELISA Kit
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