Simian ACV-A(Activin A) ELISA Kit

Rif. EK-ELK11652

Informazioni sul prodotto

• Nome: Simian ACV-A(Activin A) ELISA Kit
• Sinonimi: ACVA; Activin Beta A Beta A Homodimer
• Marchio: ELK Biotechnology
• Descrizione: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.