Human DHCR7(7-Dehydrocholesterol Reductase) ELISA Kit
Rif. EK-ELK4044
| 48T | 402,00 € | ||
| 96T | 578,00 € |
Informazioni sul prodotto
- DHCR-7; SLOS; D7SR; Smith-Lemli-Opitz Syndrome; Putative sterol reductase SR-2; Sterol Delta(7)-reductase
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DHCR7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DHCR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DHCR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DHCR7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK4044 Human DHCR7(7-Dehydrocholesterol Reductase) ELISA Kit
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