VD3(Vitamin D3) ELISA Kit
Rif. EK-ELK8102
| 48T | 332,00 € | ||
| 96T | 471,00 € |
Informazioni sul prodotto
- Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK8102 VD3(Vitamin D3) ELISA Kit
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