Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
Rif. EK-ELK9302
| 48T | 403,00 € | ||
| 96T | 577,00 € |
Informazioni sul prodotto
- TC2; Transcobalamin II
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK9302 Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
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