Mouse IL12(Interleukin 12) ELISA Kit
Rif. EK-ELK9395
48T | 520,00 € | ||
96T | 738,00 € |
Informazioni sul prodotto
- IL-12;Interleukin 12; IL12/P70; Interleukin-12/P70
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK9395 Mouse IL12(Interleukin 12) ELISA Kit
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