M-MLV (Recombinant)
Rif. SR-76516
| 5000units | 154,00 € | ||
| 10000units | 260,00 € |
Informazioni sul prodotto
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA
strand.
This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.
The enzyme has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ to 5’ exonuclease and reduced RNaseH activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNa synthesis up to 10kb long.
Proprietà chimiche
Richiesta tecnica su: SR-76516 M-MLV (Recombinant)
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