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Substrate for plasmin and microplasmin. Microplasmin hydrolyzed D-VLK-pNA with a Km of 0.361 +/- 0.017 mM and a kcat of 40.3 +/- 3.3 s-1, whereas native plasmin had a Km of 0.355 +/- 0.002 mM and a kcat of 27.9 +/- 0.3 s-1 (50 mM Tris-HCl, pH 7.4, 0.1 M NaCl, 37 C).
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