(5S)-N-(5-Amino-1-carboxypentyl) iminodiacetic acid hydrate

CAS: 941689-36-7

Ref. 3D-FA17395

Product Information

• Name: (5S)-N-(5-Amino-1-carboxypentyl) iminodiacetic acid hydrate
• Synonyms: N2,N2-Bis(carboxymethyl)-L-lysine hydrateLysine-N,N-diacetic acidAB-NTA
• Brand: Biosynth
• Description: (5S)-N-(5-Amino-1-carboxypentyl) iminodiacetic acid hydrate is a sophisticated chelating agent, designed for precise control in various biochemical applications. The reactive moieties of this compound are particularly useful in affinity chromatography and surface functionalization, making it a valuable tool in both protein purification and the development of biosensors or other biotechnological applications. Affinity chromatography: As a metal-chelator, it is particularly adept at binding divalent metal cations such as Ni²⁺, Co²⁺, and Cu²⁺, which are instrumental in stabilizing protein structures or facilitating enzyme activity. When complexed with Ni²⁺ (nickel) ions (5S)-N-(5-Amino-1-carboxypentyl) iminodiacetic acid forms a surface that can specifically capture proteins engineered with a polyhistidine tag (His-tag). Such interactions facilitate the purification and isolation of target proteins with high specificity, allowing immobilization in a controlled orientation without compromising functional integrity.Surface functionalization: The free amine group allows for conjugation via strong covalent bonds to surfaces such as glass, silica, gold (e.g., electrodes), polymers, hydrogels and magnetic beads. This creates metal-functionalized surfaces for biospecific interactions with His-tagged proteins. Examples of potential applications of this include Surface Plasmon Resonance (SPR), fluorescence microscopy and use in artificial cell systems with hydrogel-based cell-mimicking environments. Thus giving (5S)-N-(5-Amino-1-carboxypentyl) iminodiacetic acid a broad scope in its application in advanced biochemical assays and materials science.

Chemical properties

• Molecular weight: 262.26 g/mol
• Formula: C₁₀H₁₈N₂O₆·xH₂O
• Purity: Min. 95 Area-%
• Color/Form: White Powder