6-Carboxytetramethylrhodamine succinimidyl ester

CAS: 150810-69-8

Ref. 3D-FC45650

Product Information

• Name: 6-Carboxytetramethylrhodamine succinimidyl ester
• Synonyms: 6-TAMRA SE
• Brand: Biosynth
• Description: 6-Carboxytetramethylrhodamine (TAMRA) is a widely employed fluorescent dye (orange-red) suitable for different uses. In PCR applications, it has been used both as a reporter at the 5’ or as a quencher at the 3’ end. TAMRA-labelled oligonucleotides have been used to study interactions with DNA or DNA/proteins interactions. It is an excellent fluorescent acceptor for FRET applications with a suitable dye pair. It is used as a protein/antibody labelling agent for structural studies, immunochemistry and flow cytometry. Bioconjugation is generally performed through coupling of the carboxylic acid to a primary amine at the N-terminus of a protein, on lysine side chains or amino-modified oligonucleotide. Carboxylic acids activated with succinimidyl esters react with primary amines.

Chemical properties

• Molecular weight: 527.52 g/mol
• Formula: C₂₉H₂₅N₃O₇
• Purity: Min. 95 Area-%
• Color/Form: Powder