6-Carboxytetramethylrhodamine succinimidyl ester

CAS: 150810-69-8

Ref. 3D-FC45650

Product Information

• Name: 6-Carboxytetramethylrhodamine succinimidyl ester
• Synonyms: 6-TAMRA SE, 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate, 6-Carboxytetramethylrhodamine N-succinimidyl ester, 6-Carboxytetramethylrhodamine, Succinimide Ester, 6-TAMRA-NHS ester, 6-Tamra Se, Xanthylium, 9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-3,6-bis(dimethylamino)-, inner salt, 6-CARBOXYTETRAMETHYLRHODAMINE, SUCCINIMIDYL ESTER

Description

6-Carboxytetramethylrhodamine (TAMRA) is a widely employed fluorescent dye (orange-red) suitable for different uses. In PCR applications, it has been used both as a reporter at the 5’ or as a quencher at the 3’ end. TAMRA-labelled oligonucleotides have been used to study interactions with DNA or DNA/proteins interactions. It is an excellent fluorescent acceptor for FRET applications with a suitable dye pair. It is used as a protein/antibody labelling agent for structural studies, immunochemistry and flow cytometry. Bioconjugation is generally performed through coupling of the carboxylic acid to a primary amine at the N-terminus of a protein, on lysine side chains or amino-modified oligonucleotide. Carboxylic acids activated with succinimidyl esters react with primary amines.

• Brand: Biosynth

Chemical properties

• Molecular weight: 527.52 g/mol
• Formula: C₂₉H₂₅N₃O₇
• Purity: Min. 95 Area-%
• Color/Form: Powder