3,3',5,5'-Tetramethylbenzidine

CAS: 54827-17-7

Ref. 3D-FT05799

Product Information

• Name: 3,3',5,5'-Tetramethylbenzidine
• Synonyms: TMB3,3',5,5'-Tetramethylbenzidine, 1'-Biphenyl)-4,4'-Diamine,3,3',5,5'-Tetramethyl-(, 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[1, 3,3',5,5'-Tetramethyl benzidine, 3,3',5,5'-Tetramethylbenzidin, 3,3',5,5'-Tetramethylbiphenyl-4,4'-Diamine, 3,3',5,5'-Tetrametilbencidina, 3,3,5,5-Tetramethylbenzidine, 3,3′,5,5′-Tetramethyl-4,4′-diaminobiphenyl, 3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine, 3,3′,5,5′-Tetramethylbiphenyl-4,4′-diamine, 3,5,3',5'-Tetramethylbenzidine, 3,5,3′,5′-Tetramethylbenzidine, 3355Tmb, 4,4'-Bi-2,6-Xylidine, 4,4'-Diamino-3,3',5,5'-Tetramethylbiphenyl, BM blue, Benzidine, 3,3',5,5'-tetramethyl-, Benzidine, 3,3′,5,5′-tetramethyl-, ColorBurst Blue, Enhanced K-Blue, K-Blue, K-Blue Max, N-(3,5-dimethylphenyl)-3,5-dimethylaniline, Potassium-Blue Max, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate, Tetramethylbenzidine, Tmb Quick, [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-, [1,1′-Biphenyl]-4,4′-diamine, 3,3′,5,5′-tetramethyl-
• CAS: 54827-17-7

Description

TMB is commonly used as a chromogenic substrate for horse radish peroxidase for detection purposes in assay formats, such as enzyme-linked immunosorbent assays (ELISAs). TMB is a colourless solution that changes to blue upon oxidation. In the ELISA format, a stopping solution (such as sulfuric acid) is typically added to a TMB-containing solution to prevent extensive colour generation. After addition of the stop solution, the TMB solution changes from blue through to yellow (450 nm), generating a product that yields a high molar absorptivity. TMB chromogenic substrate is selected for its high sensitivity and stability.

• Brand: Biosynth

Chemical properties

• Molecular weight: 240.34 g/mol
• Formula: C₁₆H₂₀N₂
• Purity: Min. 99.0 Area-%
• Color/Form: White Powder