Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate

CAS: 84806-27-9

Ref. TR-A634000

Product Information

• Name: Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate
• Synonyms: azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid, 7-Fluorobenzofurazane-4-Sulfonic Acid Ammonium Salt, 7-Fluorobenzo-2-Oxa-1,3-Diazole-4-*Sulfonic Acid, 7-Fluoro-2,1,3-Benzoxadiazole-4-Sulfonic Acid Ammoniate (1:1)

Description

Stability Light Sensitive, Hygroscopic
Applications A water soluble bifunctional fluorogenic reagent for the sensitive and specific detection of thiols in biological specimens or proteins.SBD-F has high reactivity to thiol compounds to attain low detection limits; no flourescence in itself; biological buffer solublility; stability at RT in water for >1 week. The optimal condition for reaction was 60oC and pH 9.5 for 1 h.Please refer to the following references for more specific information regarding the application of SBD-F as a fluorogenic reagent for detection of cysteine containing peptides.Fluorescence: max Abs. l = 380 nm, max Em. l = 515 nm; Molar Abs. >4x103 (ca. 317nm)
References Imai, K., et al.: Anal. Biochem., 128, 471 (1983); Sueyoshi, T., et al.: J. Biochem., 97, 1811 (1985), Lagana, A., et al.: Anal. Biochem., 215, 266 (1993); Singh, B.K., et al.: J. Liq. Chromatogr., 17, 4469 (1994); Bishop, A.P., et al.: Free Radical Biol. Med., 18, 617 (1995);

• Brand: TRC

Chemical properties

• Molecular weight: 235.19
• Formula: C₆H₃FN₂O₄S·H₃N
• Color/Form: Neat
• InChI: InChI=1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3
• SMILES: O=S(=O)([O-])c1ccc(F)c2nonc12.[NH4+]