Soluções Tampão de pH

<p>A ready-to-use 2X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. <br> Role of 2X SDS PAGE Sample buffer :<br> 1 .Tris-HCl is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.<br> 2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.<br> 3. The high density of glycerol ensures the sample moves down into the well.<br> 4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.<br> 5. The dye visually indicates the location (tracking) of the sample in the gel. </p>

<p>RIPA (Radio-Immuno Precipitation Assay) Buffer is a ready- to-use versatile solution and widely used lysis buffer for preparing cell lysates for a variety of protein-related applications. Radio Immuno Precipitation Assay buffer (RIPA buffer) is used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structuresProtein lysis can be completed within 45 to 60 minutes. </p>

<p>DNA Methylation reagent plays a key role in DNA methylation analysis. It works by selectively converting unmethylated cytosines to uracil, while leaving methylated cytosines unchanged. This difference in conversion allows researchers to identify and analyze DNA methylation patterns using subsequent PCR amplification and sequencing.</p>

<p>DNA Quick Extraction Reagent is used to isolate genomic DNA from various samples. The reagent efficiently extracts DNA from sources like animal &amp; plant tissues, yeast, and bacteria. The process typically takes 10-30 minutes, with DNA recoveries ranging from 70-100%.</p>

<p>20X Borate Buffer is a stock solution that is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at alkaline pH. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Borate Buffer pH remains 8.5, when diluted to 1X with water.</p>