CAS 1638544-48-5

Pyrano[3,2-g:5,6-g']diquinolin-13-ium, 6-[2-carboxy-4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-1,2,2,10,10,11-hexamethyl-4,8-bis(sulfomethyl)-, inner salt

Beschreibung:

AF 594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy. AF 594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9. AF 594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.

Formel: C₃₉H₃₇N₃O₁₃S₂

Synonyme:

• Pyrano[3,2-g:5,6-g']diquinolin-13-ium, 6-[2-carboxy-4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-1,2,2,10,10,11-hexamethyl-4,8-bis(sulfomethyl)-, inner salt
• AF594 NHS ester, 5-isomer
• AF594-NHS, 5-isomer
• BP Fluor 594 NHS Ester

BP Fluor 594 NHS Ester

CAS: 1638544-48-5

BP Fluor 594 NHS Ester

Formel: C₃₉H₃₇N₃O₁₃S₂

Reinheit: 70%

Molekulargewicht: 819.87

Ref: 54-BIPG1938

BP Fluor 594 NHS ester

CAS: 1638544-48-5

BP Fluor 594 NHS ester is a widely used tool for modifying proteins or antibodies through the labeling of primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling process is optimal at pH 7-9, forming stable covalent amide bonds. BP Fluor 594 dye supports high molar ratio protein labeling with negligible self-quenching, resulting in brighter conjugates and more sensitive detection. BP Fluor 594 is a bright, water-soluble, pH-insensitive red fluorescent dye effective across a pH range of 4 to 10, with absorption and emission peaks at 590 and 617 nm, respectively. It is compatible with 561 nm and 594 nm laser lines. BP Fluor 594 can bind to various antibodies, peptides, proteins, tracers, and amplification substrates, and it is commonly used to generate stable signals in imaging and flow cytometry.

Formel: C₃₉H₃₇N₃O₁₃S₂

Farbe und Form: Solid

Molekulargewicht: 819.853

Ref: TM-T206110