CAS 367-93-1

Isopropil β-D-tiogalactopiranósido

Descripción:

Isopropil β-D-tiogalactopiranósido (IPTG) es un compuesto sintético comúnmente utilizado en biología molecular como un inductor de la expresión génica. Es un galactósido, específicamente un tiogalactósido, lo que significa que contiene un átomo de azufre en lugar del oxígeno en la parte de galactosa. IPTG es estructuralmente similar a la lactosa y es reconocido por el sistema del operón lac en bacterias, particularmente Escherichia coli, donde se une al represor lac, lo que lleva a la activación de genes bajo el control del promotor lac. Esta propiedad hace que IPTG sea una herramienta valiosa para los investigadores en estudios de expresión de proteínas. El compuesto se suministra típicamente como un polvo cristalino blanco y es soluble en agua, lo que facilita la preparación de soluciones para uso experimental. IPTG es estable bajo una variedad de condiciones, aunque debe almacenarse en un lugar fresco y seco para mantener su integridad. Su uso es generalizado en clonación y tecnología de ADN recombinante, facilitando la producción de proteínas para aplicaciones de investigación y terapéuticas.

Fórmula: C₉H₁₈O₅S

InChI: InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

Clave InChI: InChIKey=BPHPUYQFMNQIOC-NXRLNHOXSA-N

SMILES: S(C(C)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

Sinónimos:

• 1-Isopropylthio-β-D-galactopyranoside
• 1-Methylethyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Galactopyranoside, isopropyl 1-thio-
• Galactopyranoside, isopropyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Galactopyranoside, isopropyl 1-thio-, β-D-
• Iptg
• Isopropyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Isopropyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• Isopropyl 1-thio-β-D-galactopyranoside
• Isopropyl 1-thio-β-D-galactoside
• Isopropyl thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Isopropyl thio-β-<span class="text-smallcaps">D</span>-galactoside
• Isopropyl thio-β-D-galactopyranoside
• Isopropyl thio-β-D-galactoside
• Isopropyl β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• Isopropyl β-D-thiogalactopyranoside
• Isopropyl β-thiogalactopyranoside
• Isopropyl β-thiogalactoside
• Isopropyl-β-<span class="text-smallcaps">D</span>-1-thiogalactopyranoside
• Isopropyl-β-D-thiogalaktopyranosid
• Thioisopropyl β-<span class="text-smallcaps">D</span>-galactopyranoside
• Thioisopropyl β-D-galactopyranoside
• isopropil-β-D-tiogalactopiranosida
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 1-methylethyl 1-thio-
• Isopropylthio-beta-Thiogalactosidase
• Isopropyl-beta-D-thiogalactoside
• β-D-Galactopyranoside, 1-methylethyl 1-thio-
• Isopropyl β-D-1 Thiogalactopyranoside
• Isopropyl β-D-thiogalactoside
• Isopropyl-1-thio-beta-D-galactopyranoside
• HEMIDIOXANE ADDUCT
• Isopropylthio-beta-galactoside
• Isopropyl thiogalactoside
• 1-Methylethyl 1-thio-β-D-galactopyranoside
• Isopropylthio-B-thiogalactopyranoside
• Isopropyl-beta-D-thiogalactopyranoside, Dioxane Free
• 1-methylethyl 1-thio-D-galactopyranoside

Isopropyl-β-D-thiogalactopyranoside, <5ppm dioxane

CAS: 367-93-1

IPTG is a gratuitous beta-D-galactosidase inducer. In Escherichia coli IPTG functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repressor binding to the operator of the lac operon and thus the repression of the beta-galactosidase coding gene lacZ. IPTG is a commonly used reagent in cloning procedures that require induction of beta-galactosidase activity and is used in conjunction with X-Gal or Lapis-β-D-Gal in blue-white color selection of recombinant bacterial colonies.

Fórmula: C₉H₁₈O₅S

Pureza: Min. 99.0 Area-%

Peso molecular: 238.30 g/mol

Ref: 3D-I-8000

Isopropyl-β-D-thiogalactopyranoside, approx. 15.0% dioxane

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Fórmula: C₉H₁₈O₅S

Peso molecular: 238.30 g/mol

Ref: 3D-I-8050

Isopropyl-β-D-thiogalactopyranoside, <5ppm dioxane, plant origin (ex peach gum)

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Fórmula: C₉H₁₈O₅S

Pureza: Min. 99.0 Area-%

Peso molecular: 238.30 g/mol

Ref: 3D-I-8070

Isopropyl-B-D-Thiogalactopyranoside (IPTG) (dioxan-free) for MB, 99%

CAS: 367-93-1

Fórmula: C₉H₁₈O₅S

Pureza: min. 99%

Forma y color: White, Crystalline powder

Peso molecular: 238.31

Ref: SR-67208

Isopropyl-B-D-Thiogalactopyranoside (IPTG) (dioxan free), 99%

CAS: 367-93-1

Fórmula: C₉H₁₈O₅S

Pureza: min. 99%

Forma y color: White, Crystalline powder

Peso molecular: 238.31

Ref: SR-54110

IPTG, 1M aqueous solution

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Fórmula: C₉H₁₈O₅S

Pureza: Min. 95%

Forma y color: Clear Liquid

Peso molecular: 238.3 g/mol

Ref: 3D-EI184219