Sustratos fluorogénicos

<p>Methyl 4-methylumbelliferyl hexyl phosphonate is a lipase suicide inhibitor that is a fluorogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases and the fluorescent signal can be detected at Ex 363 nm and Em 465 nm.</p>

<p>3-Carboxyumbelliferyl b-D-glucuronide serves as a highly sensitive fluorogenic substrate for detecting b-glucuronidase activity. Upon enzymatic cleavage, it generates a fluorescent product enabling easy and accurate monitoring of enzyme activity in various applications, such as diagnostics, drug discovery, and research.</p>

<p>A fluorogenic MOSCERDAM® substrate targeting cellulases</p>

<p>5-(Pentafluorobenzoylamino)fluoroscein di-b-D-galactopyranoside, commonly referred as PFB-FDG or PFB-FDGal, is a cell-permeable enzyme substrate for β-galactosidase used in live cell imaging assays. After cleavage, 5-(pentafluorobenzoylamino)fluorescein (PFB-F) is released, generating a green fluorescence (Em ~520 nm)) of a higher intensity than fluorescein. Its main use is for bacterial detection (e.g. E. coli LacZ assays), but also for flow cytometry analytical assays.</p>

<p>Resorufin benzyl ether is a chemical compound that can be used as a substrate for cytochrome P450. It has been shown to inhibit the activity of epoxide hydrolase, an enzyme involved in the metabolism of endoperoxides. Resorufin benzyl ether has also been found to inhibit cytochrome P450 enzymes, which are responsible for oxidative metabolism of xenobiotics. This compound has been shown to have a high affinity for mouse monoclonal antibody and is used in immunoassays to detect mouse antibodies. Resorufin benzyl ether is used in biochemical assays and polymerase chain reactions (PCRs) as a fluorescence marker. In addition, this compound is used in model systems to study enzyme activities such as recombinant cytochrome p450 and protein oxidation by reactive oxygen species (ROS).</p>

<p>Fluorogenic substrate targeting plasmin</p>

<p>D-Alanine 7-amido-4-methylcoumarin trifluoroacetate salt is a chromogenic substrate for the enzyme Beta-galactosidase. It is used as a diagnostic tool in the detection of certain bacteria, such as Enterobacteriaceae and Clostridium perfringens. It can also be used to detect the presence of beta galactosidase in food products and environmental samples. D-Alanine 7-amido-4-methylcoumarin trifluoroacetate salt has an applications in chemiluminescence, bioluminescence, and fluorescence. This product is not intended for use as a drug or food additive.</p>

<p>4-Methylumbelliferyl-b-D-xylotrioside is a fluorogenic substrate that reacts with an enzyme in the presence of oxygen to produce light. It is used in diagnostic applications, such as detecting the presence of a specific enzyme or protein. 4-Methylumbelliferyl-b-D-xylotrioside can also be used as a chromogenic substrate for the detection of DNA and RNA. This compound can be conjugated with other molecules for use in immunoassays and other diagnostic applications. 4-Methylumbelliferyl-b-D-xylotrioside has been shown to bind to bacterial cells and activate them, which leads to the production of light through bioluminescence.</p>

<p>4-Methylumbelliferyl 2-azido-2-deoxy-4,6-O-phenylmethylene-a-D-galactopyranoside is a fluorogenic substrate that reacts with beta D galactosidase to produce light. This product is useful for diagnostics, conjugate production, and other applications. 4MUF 2 -Gal provides a convenient means of detecting beta D galactosidase activity in cell culture media and can be used as a chromogenic substrate for the detection of enzymes involved in glycosylation reactions. It is also used to detect staining by bioluminescence. The compound can be used in food testing and environmental testing to measure the presence of beta D galactosidase.</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-b-D-glucopyranoside is a fluorogenic substrate specifically designed for the detection and quantification of hexosaminidase enzymes. Upon cleavage by the target enzyme, it generates a bright fluorescent signal facilitating the investigation of enzyme activity, screening of inhibitors, and diagnostics.</p>

<p>4-Methylumbelliferyl-phosphoethanolamine is a phosphorylated substrate that can be used in the detection of enzymes such as alkaline phosphatase, esterase, and lipase. This compound has been shown to be a fluorescent and chromogenic substrate for many diagnostic purposes. 4-Methylumbelliferyl-phosphoethanolamine is also used as a fluorogenic substrate for chemiluminescent reactions that are found in culture media and environmental testing.</p>

<p>3-HTC reacts reversibly with thiols and disulfides, and can be used to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates.(λmax: 448 nm in its reduced thiolate form, and a λmax 370-410 nm for the oxidized mixed disulfide).</p>

<p>4-Methylumbelliferyl 3-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is an organic compound that is used as a radioactive marker for DNA. It can be synthesized in the laboratory and its use allows for the tracking of DNA molecules. The production of this marker is achieved by recombinant expression of the gene encoding the enzyme β-D-galactosidase, which hydrolyzes 4-methylumbelliferyl β-D-galactopyranoside to release 4-methylumbelliferone and α-L-fucopyranose. The enzyme uses UDP glucose as a cofactor to catalyse the reaction. This product has been isolated from Corynebacterium glutamicum, with recombinant expression vectors used to produce it in host cells such as Escherichia coli or Corynebacterium glutamicum. It has been found that</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-azido-2-deoxy-a-D-galactopyranoside is a fluorogenic substrate for the enzyme beta galactosidase. It is used in food testing, environmental testing, and diagnostics. 4MUF3A2DGAL is an enzyme substrate that provides high quality chemiluminescence and bioluminescence signals. This product has a CAS number of 124167-43-7 and is available in both high purity and conjugate forms.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis (deficiency of the enzyme alpha-L-fucosidase) and glycoprotein metabolism disorder detection.</p>

<p>3-Carboxyumbelliferyl b-D-galactopyranoside is a fluorescent probe that can be used to study protein synthesis in live cells. It binds to the active site of the enzyme peptidyl transferase, which catalyzes the covalent linkage of amino acids to form a polypeptide chain. 3CMBG is specific for the amino acid proline and is a substrate for peptidyl transferase. The fluorescence of 3CMBG increases when it is bound to peptidyl transferase, making it useful as a marker for protein synthesis.</p>

<p>4-Methylumbelliferyl b-L-arabinopyranoside is a chemiluminescent substrate that is used in the detection of various enzymes and other proteins. It can be used as a fluorogenic substrate, chromogenic substrate, or enzyme substrate. 4-Methylumbelliferyl b-L-arabinopyranoside has been shown to be an effective probe for detecting catalase, cytochrome c oxidase, and peroxidase activity. This product also has applications in diagnostics and food testing.</p>

<p>6,8-Difluoro-4-methylumbelliferyl b-D-glucuronide is a chemiluminescent substrate for the enzyme glucuronidase. The compound has been shown to be stable in the presence of a wide range of pHs and temperatures. It can be used in applications such as diagnostics, culture media and food testing. 6,8-Difluoro-4-methylumbelliferyl b-D-glucuronide has a high purity level and can be used as an indicator for other compounds such as acetaminophen or ibuprofen.</p>

<p>4-Methylumbelliferyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a fluorescent marker for nucleic acid molecules. It can be applied to identify the metabolic pathways of bacteria in an ecosystem by using fluorescence microscopy. 4-Methylumbelliferyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is also used to measure the activity of some enzymes in vitro. This compound has been shown to be effective against spittlebugs and other pests that feed on plant sap. The toxic effect of this compound is due to its ability to inhibit acetate synthesis and disrupt membrane transport systems.</p>

<p>Resorufin oleate is a fluorogenic lipase substrate.</p>