Otros

<p>Applications A metabolite of Furazolidone and Nitrofuran.Environmental contaminants; Food contaminants; Heat processing contaminants<br>References Mir, G., et al.: Indian J. Anim. Sci., 54, 888 (1984), Gottschall, D., et al.: J. Agric. Food Chem., 43, 2520 (1995)<br></p>

<p>5-Bromo-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in lysosomal storage disorder screening such as for Sanfilippo syndrome.</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-galactosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is used in lactose intolerance screening.</p>

<p>A substrate for alkaline phosphatase yielding a blue insoluble product upon cleavage. Colour can be further enhanced when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. The micronized structure enhances solubility.</p>

<p>Naphthol AS-OL is a widely used chromogenic enzyme substrate that forms a colored product upon reaction with specific enzymes. It is commonly employed in histochemical and cytochemical staining techniques to visualize enzyme activity in cells and tissues. Naphthol AS-OL is particularly useful for detecting the presence of enzymes such as alkaline phosphatase, acid phosphatase, and esterases. The resulting colored product can be easily observed under a microscope, allowing researchers to study the distribution and localization of these enzymes in various biological samples.</p>

<p>4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.</p>

<p>2-Chloro-4-nitrophenyl b-D-maltoheptaoside is a fluorescent compound that is used in medical research for the visualization of muscle glycogen. It is also used to study the binding of molecules to proteins, as well as to measure enzyme activity. The compound has been shown to be insensitive to changes in pH and temperature, so it can be used at different pH levels and temperatures without affecting its function. 2-Chloro-4-nitrophenyl b-D-maltoheptaoside can be detected by titration calorimetry due to its high molecular weight. The molecule's affinity for carbohydrates also allows it to be used in optimization studies of carbohydrate molecules.</p>

<p>Aldol® 515 4-acetoxybutyrate solution, 0.75 M in DMSO, is a chromogenic substrate that can be used to measure the activity of aldolase. It is a liquid at room temperature and has a pH of 7.2. The Aldol® 515 4-acetoxybutyrate solution, 0.75 M in DMSO, is soluble in water and organic solvents such as DMSO and ethanol. This product does not contain any preservatives or additives and is suitable for use with humans and animals. Aldol® 515 4-acetoxybutyrate solution, 0.75 M in DMSO, is manufactured by Cymit Quimica under patents EP 2427431 and US 8940909.br&gt;br&gt;<br>Aldolase catalyzes the conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate</p>

<p>4-(Hydroxymethyl)phenyl-beta-D-glucopyranoside tetraacetate is a drug that has been shown to have antidiabetic effects in diabetic patients. It has been shown to inhibit tyrosinase activity and increase the production of gamma-aminobutyric acid (GABA) in brain cells. This compound also has a protective effect against glutamate toxicity, and is being studied as a potential treatment for epilepsy. 4-(Hydroxymethyl)phenyl-beta-D-glucopyranoside tetraacetate inhibits the production of tyrosinase, which is an enzyme responsible for the conversion of tyrosine to melanin. This process can be inhibited by inhibiting tyrosinase activity with 4-(hydroxymethyl)phenyl-beta-D-glucopyranoside tetraacetate.</p>

<p>Aldol® 515 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 515 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 515 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in ?-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>5-Bromo-4-chloro-3-indolyl 5-O-feruloyl a-L-arabinofuranoside is a chemiluminescent ligand that is used in the detection of DNA, RNA, and proteins. It has been shown to be an efficient fluorescence substrate for DNA and RNA polymerases. 5-Bromo-4-chloro-3-indolyl 5-O-feruloyl a-L-arabinofuranoside is suitable for use in diagnostic tests for bacteria, fungi, viruses, parasites, and toxins. This compound has been extensively tested for toxicity and carcinogenicity.</p>

<p>Substrate for beta-galactosidase</p>

<p>1-Naphthyl b-D-glucuronide sodium salt is a chromogenic substrate commonly used to assay the activity of beta-glucuronidase enzymes. It is hydrolyzed by beta-glucuronidase to yield a yellow-colored product, 1-naphthol, which can be quantified spectrophotometrically. This substrate is widely used in diagnostic tests for detection of beta-glucuronidase activity, particularly in clinical microbiology for the identification of E. coli and other enteric bacteria. With its sodium salt form, it boasts increased solubility in aqueous solutions, ensuring seamless integration into various experimental settings.</p>

<p>5-Bromo-6-chloro-3-indoxyl butyrate is an enzyme substrate that is used for the detection of bacteria and fungi in environmental testing, food testing, and diagnostics. It is also used as a fluorogenic substrate in bioluminescence and chemiluminescence assays. 5-Bromo-6-chloro-3-indoxyl butyrate binds to proteins on the cell surface, causing an increase in fluorescence when excited by light of a specific wavelength. The conjugate can be detected using a variety of methods, including staining with dyes such as safranin or methylene blue. This product has high purity and high quality.</p>

<p>Chromogenic substrate for beta-glucosidase</p>

<p>5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt anhydrous is a potent inhibitor of histidine decarboxylase (HDC). It is used in the histochemical staining of dehydrogenase and effector proteins in urine samples. This compound has been shown to inhibit the growth of macroalgae and can be used as a selective agent for phylogenetic tree construction. 5BDG,Sr with its high specificity for HDC, also inhibits cell proliferation in plant cells and ovules.</p>

<p>Aldol® 470 L-alanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-alanine aminopeptidase activity. The colorless enzyme substrate is cleaved by L-alanine aminopeptidase, yielding orange-yellow coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 470 L-alanine amide can also be used in enzymatic assays or within live bacterial cultures. Aldol® 470 L-alanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Chromogenic substrate to visualize the activity of alpha-D-mannopyranoside; red color produced</p>

<p>Histochemical substrate for esterase</p>

<p>Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.</p>

<p>4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.</p>

<p>Phosphatase substrate</p>

<p>5-Bromo-6-chloro-3-indolyl-2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate that is used as an indicator in diagnostic tests. This compound reacts with an oxidizing agent, such as hydrogen peroxide, to generate light. It is also used as a chromogenic substrate for the detection of bioluminescence and it can be applied in culture media or food testing. 5Bromo-6Chloro-3Indolyl2Acetamido2DeoxyA1DGlucopyranoside has a CAS number, which means that it meets the standards set by the Chemical Abstracts Service. It also has a high purity and quality, making it suitable for use in laboratories.</p>

<p>2-Chloro-4-nitrophenyl β-D-cellotrioside is a chromogenic substrate use in cellulase activity assays. This enzyme, which is responsible to break down cellulose, hydrolyzes 2-chloro-4-nitrophenyl beta-D-cellotrioside and releases 2-chloro-4-nitrophenol than can be quantified spectrophotometrically. The use of 2-chloro-4-nitrophenyl beta-D-cellotrioside as substrate for enzymatic assays allows detecting bacterial cellulose in urine tract infections in the biofilm state.</p>