Enzimas

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn NcoI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity NcoI binds at the recognition sequence CCATGG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding NcoI gene from Nocardia carolina

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn SmaI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity SmaI binds at the recognition sequence CCCGGG and cleaves these sequences. It creates blunt ends. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is derived from the E.coli strain encoding SmaI gene from Serratia marcescens

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn PstI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity PstI binds at the recognition sequence CTGCAG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm methylation but Inhibition by CpG methylation. It is derived from the E.coli strain encoding PstI gene from Providencia stuartii

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn XhoI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity XhoI binds at the recognition sequence CTCGAG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm methylation but Inhibition by CpG methylation. It is derived from the E.coli strain encoding XhoI gene from Xanthomonas holcicola

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn BamHI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity BamHI binds at the recognition sequence GGATCC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding BamHI gene from Bacillus amyloliquefaciens H

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn KpnI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity KpnI binds at the recognition sequence GGTACC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding KpnI gene from Klebsiella pneumoniae OK8

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn NdeI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity NdeI binds at the recognition sequence CATATG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding NdeI gene from Neisseria denitrificans

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn NotI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity NotI binds at the recognition sequence GCGGCCGC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm methylation but Inhibition by CpG methylation. It is derived from the E.coli strain encoding NotI gene from Nocardia otitidis-caviarum

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn SalI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity SalI binds at the recognition sequence GTCGAC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is derived from the E.coli strain encoding SalI gene from Streptomyces albus G

Source: Sphaerotilus species 5'-AAT↓ATT-3' 3'-TTA↑TAA-5'

HpaI is an endonuclease enzyme isolated from strains of Haemophilus parainfluenzae, recombinant, E. coli and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is GTTACC, which, as the complementary sequence is CAATGG, has rotational symmetry.

Isoschizomers: BfuCI, Bsp143I, BstENII, BstMBI, DpnII, Kzo9II, NdeII Neoschizomers: BstKTI Source: Streptomyces species 5'-↓GATC -3' 3'- CTAG↑-5'

DraI is an endonuclease enzyme isolated from strains of Deinococcus radiophilus having the DraI gene, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme.It recognizes the following sequence and produces a blunt end.

HindIII is an endonuclease enzyme isolated from strains of Haemophilus influenzae Rd, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is AAGCTT, which, as the complementary sequence is TTCGAA, has rotational symmetry.

NcoI is an endonuclease enzyme isolated from strains of Nocardia corallina and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is CCATGG which, as the complementary sequence is GGTACC, has rotational symmetry.

PvuII is an endonuclease enzyme isolated from strains of Proteus vulgaris, recombinant, E. coli, and is part of the restriction modification system.In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is CAGCTG which, as the complementary sequence is GTCGAC, has rotational symmetry.

BglI is an endonuclease enzyme isolated From strains of Bacillus globigii, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme.The enzyme recognizes internal nucleotides of the SfilI recognition site. It cuts similarly.

BioLit QuickRxn EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C ( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration : 20U/mcl