Sustratos de enzimas

Chromogenic substrate for β-D-glucosidase yielding a magenta precipitate. Differentiation of Salmonella spp. (negative) from other Enterobacteriaceae.

Histochemical substrate for alkaline phosphatase; Dark-blue color

4-Methylumbelliferyl-alpha-L-arabinofuranoside is a nucleic acid molecule that has an average diameter of 10 nm. It is used as a tracer in electron microscopy to detect the presence of nucleic acid molecules in cells. This compound is also used to study the interactions between DNA and RNA molecules by labeling the DNA strand with 4-methylumbelliferyl-alpha-L-arabinofuranoside, which contains an alpha-(2,4,6) arabinofuranosyl group. The recombinant expression of this compound can be carried out in corynebacterium glutamicum, and it has a bulk density that ranges from 1.7 to 2.1 g/cm3 and an average particle diameter ranging from 0.5 to 1 micron. It is not soluble in water or alcohol but is soluble in acetone or chloroform.

Chromogenic substrate for beta-D-galactosidase yielding a lapis colored precipitate. 5-Bromo-3-indoxyl-beta-D-galactopyranoside can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside as an alternative to 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), producing a darker blue color for detection of beta-galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue). 5-Bromo-3-indoxyl-beta-D-galactopyranoside is also used in histochemistry to demonstrate beta-galactosidase activity in tissues.

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Chlorophenol red-b-D-galactopyranoside sodium salt is the salt from of CPRG (chlorophenol red-b-D-galactopyranoside), that has a higher solubility. It is a chromogenic substrate for beta-galactosidases that, after enzymatic cleavage, releases chlorophenol red, a dark red compound quantified by absorbance at 570 nm.

Chromogenic β-glucosaminidase substrate yielding a yellow solution upon cleavage, used particularly in yeasts and molds.

DMNPE-caged Luciferin is a photoactivatable probe that can easily penetrate the cell membrane, and by exposure to UV light it undergoes a photochemical decomposition liberating a Luciferin molecule. Once exposed to Luciferase enzyme expressed by the cells, Luciferin emits visible (and measurable) light. Using_x000D_this technique, imaging of luciferase activity was performed both in living cells and in mouse tumor xenograft, by intraperitoneal injection of the caged DMNPE luciferin (Chem. Commun., 2009, 0, 4028-4030).

4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is a synthetic substrate that is used in enzyme kinetics. It is a beta-galactosidase substrate that reacts with the enzyme to form a product. The rate of reaction can be measured by following the change in absorbance over time. This product will react with an acceptor, such as chondroitin sulfate, to produce an observable reaction product. 4NPBGA has been shown to be an effective carbon source for plant physiology studies and has been shown to have optimal activity at pH 6.5.

Please enquire for more information about 5-Nitro-3-indoxyl-β-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

A chromogenic and fluorogenic substrate, used for detection of esterase activity.

Chemiluminescence beta-galactosidase substrate

Fluorogenic substrate for α-L-arabinosidase yielding a blue fluorescent solution upon cleavage.

Chromogenic α-mannosidase substrate yielding a absorbent solution upon cleavage. Also used for studies of the crystal structure of the complexes of concanavalin A and binding studies with concanavalin A.

6-Fluoro-3-indoxyl-beta-D-galactopyranoside is a fluorescent chromogenic substrate that is commonly used for the detection and quantification of beta-galactosidase activity. When this substrate is hydrolyzed by beta-galactosidase, it produces a fluorescent blue product that can be easily detected and measured using a fluorometer or spectrophotometer. This substrate is often used in microbiology and molecular biology research to study gene expression and enzyme kinetics, and is also used in medical diagnostics as a marker for specific diseases and conditions.

Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.

Chromogenic substrate for α-L-arabinofuranosidase yielding a yellow solution upon cleavage.

4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).