Sustratos de enzimas

L-Proline 7-amido-4-methylcoumarin hydrobromide salt is a fluorogenic peptide substrate for proline-specific peptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.

Chromogenic substrate for beta-galactofuranosidase

The 4-Methylumbelliferyl b-D-ribofuranoside is a top-notch fluorogenic substrate for glycosidase activity detection. Upon hydrolysis by enzymes, it generates a strongly fluorescent 4-methylumbelliferone molecule, enabling rapid assay results and real-time monitoring of enzymatic reactions. This product is suitable for many biological research applications, such as in vitro analysis, diagnostic testing, and drug discovery. With its high sensitivity and specificity, it delivers consistent and accurate results, making it an essential tool for scientists and researchers.

4-Methylumbelliferyl 3-O-(N-acetylneuraminyl)-b-D-glucopyranoside is a chromogenic substrate. It is used for the detection of glycoproteins and glycolipids in food testing, staining, environmental testing, and diagnostics. It can also be used as a fluorogenic substrate for the detection of glycoproteins and glycolipids in fluorescence microscopy. 4-methylumbelliferyl 3-O-(N-acetylneuraminyl)-b-D-glucopyranoside has been shown to be a ligand for the lectin receptor Galectin 9, which is involved in inflammation and cancer cell growth. 4-methylumbelliferyl 3-O-(N-acetylneuraminyl)-b-D-glucopyranoside has been shown to be an enzyme substrate for alkaline phosphatase, β

Chromogenic β-maltosidase substrate yielding a yellow solution upon cleavage.

4-Nitrophenyl beta-D-xylobioside is a chromogenic substrate for xylanase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-xylobioside is used in different applications such as the Xylan degradation studies, paper/pulp industry applications

<p>L-Ala-PAB-resorufin is a highly sensitive chromogenic and fluorogenic indicator for L-alanine aminopeptidase activity and related enzymes occurring in prokaryotic as well as eukaryotic organisms. After removal of the L-alanyl substructure by enzymatic hydrolysis and spontaneous elimination of the para-aminobenzyl spacer, the initially orange colored compound yields an intense pink color and red fluorescence.</p>

<p>2,4-Dinitrophenyl-beta-D-glucopyranoside is a chromogenic substrate used to assay enzymes, such as glucosidases. These enzymes catalyze the hydrolysis of glycosidic bonds in sugars.</p>

<p>Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>L-Pro-PAB-Resorufin is a highly sensitive chromogenic and fluorogenic indicator for L-proline aminopeptidase activity and related enzymes occurring in prokaryotic as well as eukaryotic organisms. After removal of the L-proline amide substructure by enzymatic hydrolysis and spontaneous elimination of the para-amino-benzyl (PAB) spacer, the initially orange colored compound yields an intense pink color. In addition, the released chromophor is an excellent fluorophor showing red fluorescence at an emission wavelength of 580 to 600 nm when excited at 560 to 570 nm.</p>

A MOSCERDAM® substrate targeting the fucosidase enzyme

<p>2-Nitrophenyl b-D-arabinopyranoside is a chemical compound that is used in the detection of ATP by chemiluminescence. It acts as a substrate for enzymes such as luciferase and catalase, which catalyze the oxidation of 2-nitrophenyl b-D-arabinopyranoside to phenol and carbon dioxide with the release of light. This reaction can be used in diagnostic applications such as detecting bacteria in culture media or diagnosing food spoilage. 2-Nitrophenyl b-D-arabinopyranoside can also be used as a conjugate to detect proteins or nucleic acids by fluorescence spectroscopy. The CAS number for this compound is 107772-06-1.</p>

Luciferin-beta-D-galactoside is an acceptor in immunoassays. It is used as a label for specific antibodies and antigens, and the light emitted by this compound can be measured with a luminometer. Luciferin-beta-D-galactoside binds to the bacterial enzyme β-galactosidase, which catalyzes the hydrolysis of lactose to form glucose and galactose. Mammalian cells have been used as a source of β-galactosidase in order to measure luciferin-beta-D-galactoside activity. The substrate specificity of this enzyme has also been studied using mammalian tissue extracts.

4-Nitrophenyl myristate (NphOMy) is a chromogenic enzyme substrate used as a diagnosis tool for detection of β-lactamase hydrolysis activities. In the presence of certain drugs, 4-nitrophenyl myristate HSA-Tyr411-catalyzed hydrolysis seems to be inhibited.

<p>2-Naphthyl-lpha-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.</p>

Luc-coliforms Na is a high quality, chromogenic substrate for the detection of coliforms. It is used widely in the food industry to detect whether coliforms are present in milk, cheese, and other dairy products. Luc-coliforms Na provides a color change from yellow to blue when it reacts with coliforms and has a high pH range of 4.0-8.0. Luc-coliforms Na has a CAS number of 93824-43-4.

Luciferin-alpha-D-glucoside, potassium salt is a fluorogenic substrate that is used in diagnostics as a ligand. It can be conjugated to various substances, including proteins and antibodies. It is also used as an enzyme substrate in the detection of glucose oxidase in bacterial culture media and chemiluminescence in food testing, environmental testing, and staining.

Singlet Oxygen Chemiluminescence Probe, SOCL

<p>Ostazin Brilliant Red H-3B Galactomannan is a chemiluminescent substrate used for the detection of β-1,4-galactomannans. It reacts with the glucose residues on the mannan backbone to produce a red fluorescent product. This product can be detected by spectrophotometry at an excitation wavelength of 395 nm and emission wavelength of 510 nm. The Ostazin Brilliant Red H-3B Galactomannan substrate is useful in detecting Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Group A streptococci, Group B streptococci, Group D streptococci, Group G streptococci, and Group C streptococci.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium salt is a fluorogenic substrate for beta-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium saltas a substrate for measuring the beta-N-acetylglucosaminidase activity is used for diagnosis of Mucopolysaccharidosis IIIB (MPS IIIB) (a rare lysosomal storage disorder) and Sanfilippo B syndrome screening.</p>