Sustratos de enzimas

<p>Applications A histochemical substrate for a-N-acetyl-galactosaminidase.<br>References Zhu, et al.: Gene, 137, 309 (1994)<br></p>

<p>Applications (S)-ETHYL 2,6-DIISOCYANATOHEXANOATE (cas# 45172-15-4) is a useful research chemical.<br> Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package<br></p>

<p>Stability Hygroscopic<br>Applications Protected Glucopyranosiduronic Acid.<br></p>

<p>Applications It was found in the root bark of Punica granatum L., Punicaceae. Used as a enzyme substrate.<br>References Hunt, R., et al.: J. Pharm. Pharmacol., 22, 29S (1970), Hashimoto, T., et al.: Plant Physiol., 100, 836 (1992), Boswell, H., et al.: Phytochemistry, 52, 855 (1999),<br></p>

<p>5-Bromo-3-indolyl a-D-galactopyranoside is a fluorogenic substrate that can be used for the detection of beta-galactosidase activity. It is also used as an enzyme substrate in diagnostics and research, as well as a chromogenic substrate in staining. 5-Bromo-3-indolyl a-D-galactopyranoside has been shown to be an excellent conjugate for proteins, nucleic acids, and other biomolecules. This product can also be used in culture media for the cultivation of bacteria and yeast.</p>

Aldol 496 acetate is a chromogenic substrate for the enzyme Aldolase. It is also used as a ligand to detect the presence of Aldolase in biological fluids and tissues. This product is designed for Food testing, Diagnostics, Chromogenic substrate, Staining, Enzyme substrate, Bioluminescence, Fluorogenic substrate, Chemiluminescence, and Culture media. Aldol 496 acetate is an organic chemical with high purity and high quality.

<p>Aldol® 515 propionate is a chromogenic substrate that is used as a reagent in food testing, diagnostics, and environmental testing. It reacts with an enzyme to form a fluorescent product that can be detected with a spectrophotometer. The substrate has been shown to have high purity and high quality.</p>

<p>6-Chloro-4-methylumbelliferyl b-D glucuronide is a fluorogenic substrate for beta-glucuronidase that exhibits longer absorption/emission maxima (~385/500 nm) than typical 4MU-derived substrates.</p>

<p>2-Nitrophenyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is a chromogenic substrate used to detect the enzymatic activity of xylosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is used in bacterial hemicellulose metabolism assays.</p>

<p>3-Carboxyumbelliferyl b-D-galactopyranoside is a fluorescent probe that can be used to study protein synthesis in live cells. It binds to the active site of the enzyme peptidyl transferase, which catalyzes the covalent linkage of amino acids to form a polypeptide chain. 3CMBG is specific for the amino acid proline and is a substrate for peptidyl transferase. The fluorescence of 3CMBG increases when it is bound to peptidyl transferase, making it useful as a marker for protein synthesis.</p>

<p>4-Methylumbelliferyl acetate is a fluorescent compound that can be used to measure the activity of cytochrome P450 enzymes. The fluorescence intensity of 4-methylumbelliferyl acetate increases when it reacts with substrates and inhibitors in vitro. This chemical has been shown to inhibit the enzyme cyclooxygenase in rat liver microsomes, which may lead to blood disorders. It also inhibits coumarin derivatives and human pathogens, such as erythromycin-resistant Staphylococcus aureus (MRSA). 4-Methylumbelliferyl acetate is used as a substrate for polymerase chain reaction (PCR) analysis because it is not susceptible to hydrolysis by esterases.</p>

<p>Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.</p>

<p>Resorufin benzyl ether is a chemical compound that can be used as a substrate for cytochrome P450. It has been shown to inhibit the activity of epoxide hydrolase, an enzyme involved in the metabolism of endoperoxides. Resorufin benzyl ether has also been found to inhibit cytochrome P450 enzymes, which are responsible for oxidative metabolism of xenobiotics. This compound has been shown to have a high affinity for mouse monoclonal antibody and is used in immunoassays to detect mouse antibodies. Resorufin benzyl ether is used in biochemical assays and polymerase chain reactions (PCRs) as a fluorescence marker. In addition, this compound is used in model systems to study enzyme activities such as recombinant cytochrome p450 and protein oxidation by reactive oxygen species (ROS).</p>

<p>Aldol® 450 acetate is an enzyme substrate that is used in the detection of aldolase activity. Aldol® 450 acetate has been shown to be a useful alternative to the traditional bioluminescence and chemiluminescence methods for detecting aldolase activity. It has also been shown to be effective as a fluorogenic substrate for detecting aldolase activity in food products. Aldol® 450 acetate can be conjugated with other compounds, such as ligands and dyes, to enable it to detect different enzymes.<br>Aldol® 450 acetate has an absorption maximum at 450 nm and fluoresces blue-green under longwave UV light excitation. It is soluble in water and exhibits excellent stability at high temperatures (up to 50°C) and pH (1-14).</p>

<p>2-Dodecylresorufin is a fluorescent probe that reacts with the hydroxyl radicals in the cell, which are generated by the enzyme superoxide dismutase. 2-Dodecylresorufin can be used to measure reactive oxygen species (ROS) and ROS-induced cellular damage. It is also used to study oxidative stress in vitro. The chemical structure of 2-dodecylresorufin contains a linker arm that provides a reversible linkage to the cell surface, allowing for extracellular measurements of reactive oxygen species (ROS). This compound has been shown to have high reactivity, making it useful for studying population dynamics and fluorescent techniques.</p>

<p>2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used to detect and measure the activity of glycosidase enzymes. Glycosidases are enzymes that hydrolyze glycosidic bonds in carbohydrates, and are produced by many organisms. The substrate is cleaved by glycosidase to release a chromophore, which can be quantified via spectrophotometry analysis. 2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the detection of NAGase activity in biological samples, such as serum, urine, and cell lysates. It is also used for the identification and characterization of glycosidases in microorganisms and the study of glycosylation-related processes in cells and tissues.</p>

<p>4-Trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid is a fluorescent chemiluminescent substrate that is used in the detection of beta-galactosidase activity. Beta-galactosidase catalyzes the hydrolysis of 4-trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid to form 4-trifluoromethylumbelliferone and D-galactose, which emits light at approximately 480 nm. This reaction can be detected by fluorescence spectrophotometry using a fluorometer or photometer. 4TFUNA is not toxic, does not inhibit enzymes, and has been tested for toxicity and mutagenicity using bacteria and mammalian cells.</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-b-D-glucopyranoside is a fluorogenic substrate specifically designed for the detection and quantification of hexosaminidase enzymes. Upon cleavage by the target enzyme, it generates a bright fluorescent signal facilitating the investigation of enzyme activity, screening of inhibitors, and diagnostics.</p>

Green fluorescent cationic dye

<p>Colchicine fluorescein is the fluorogenic form of colchicine. It is used for staining assembly of tubulin. Colchicine-site inhibitors (abbrevated as CBSIs) disrupt tubulin polymerization. Colchicine fluorescein serves as a probe to study the binding of colchicine to tubulin, as the colchicine binding site is a promising target for discovering novel antitumour agents.</p>