Sustratos de enzimas

<p>4-Methylumbelliferyl 3-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is an organic compound that is used as a radioactive marker for DNA. It can be synthesized in the laboratory and its use allows for the tracking of DNA molecules. The production of this marker is achieved by recombinant expression of the gene encoding the enzyme β-D-galactosidase, which hydrolyzes 4-methylumbelliferyl β-D-galactopyranoside to release 4-methylumbelliferone and α-L-fucopyranose. The enzyme uses UDP glucose as a cofactor to catalyse the reaction. This product has been isolated from Corynebacterium glutamicum, with recombinant expression vectors used to produce it in host cells such as Escherichia coli or Corynebacterium glutamicum. It has been found that</p>

<p>6,8-Difluoro-4-methylumbelliferyl b-D-glucuronide is a chemiluminescent substrate for the enzyme glucuronidase. The compound has been shown to be stable in the presence of a wide range of pHs and temperatures. It can be used in applications such as diagnostics, culture media and food testing. 6,8-Difluoro-4-methylumbelliferyl b-D-glucuronide has a high purity level and can be used as an indicator for other compounds such as acetaminophen or ibuprofen.</p>

<p>2-Nitrophenyl 2,3-di-O-acetyl-b-D-xylopyranoside is a chromogenic substrate used to assay the activity of xylosidase enzymes. It is hydrolyzed by xylosidase to yield a yellow-colored product, 2-nitrophenol, which absorbs light at 405 and 410 nm. This substrate is used in research to study the function of xylosidase enzymes and their role in various biological processes. It is also used in diagnostic tests for the detection of xylosidase activity in different microorganisms, including bacteria and fungi.</p>

<p>Histamine (Scombrotoxin) fish poisoning is the most frequent cause of fish poisoning incidents today. Cymit Quimica's BCM®; Histamine-producingbacteria Broth (C-0450) is a growth medium for the detection of histamine-producing bacteria in food and water samples. The medium contains an indicator that turns orange to red due to metabolites released by histidine decarboxylase.</p>

<p>3-Indolyl b-D-cellotrioside is a chemiluminescent substrate that is used for the detection of bacterial activity. 3-Indolyl b-D-cellotrioside reacts with hydrogen peroxide to produce light, which can be detected by luminometers. This product can be used in diagnostic tests to detect the presence of bacteria in food, water, and other samples. It has been shown to have excellent performance characteristics and high purity and quality.</p>

<p>4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is an advanced chromogenic pNP enzyme substrate that delivers unparalleled performance in enzymatic assays. This high-quality substrate is specifically designed for studying glycosidases and other enzymes involved in carbohydrate metabolism. Our 4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is synthesized with the highest quality materials to provide incredible accuracy and reliability in your research applications.</p>

<p>D-Ile-Pro-Arg p-nitroanilide dihydrochloride is a chromogenic substrate commonly used in research to assess the activity of various enzymes. It has been utilized in studies related to thrombin inhibition, enzyme assays of protease enzymes, activation and enzymatic activity analysis of Factor VII-activating protease, and as a substrate for measuring glandular kallikrein activity. This substrate has been employed in assays to study the amidolytic activity of enzymes such as thrombin, plasma kallikrein, plasmin, and other proteases.</p>

5-Bromo-4-chloro-3-indoxyl nonanoate (5BCNI) is a chromogenic substrate for the detection of alkaline phosphatase. It is soluble in most organic solvents and is stable at room temperature for up to 30 days. 5BCNI has been shown to be a high quality, sensitive and specific substrate for the detection of alkaline phosphatase in culture media, diagnostics, conjugates, environmental testing and chemiluminescence. It has also been used as an enzyme substrate in bioluminescence studies. The CAS number for 5BCNI is 133950-77-3.

<p>Resorufin pentyl ether is a drug transporter that can be used as an experimental model for studying the effects of chemical pesticides on humans. Resorufin pentyl ether inhibits energy metabolism and inhibits hepatic p450 activity and polymerase chain reaction in hepatocyte-like cells in vitro. It has been shown to inhibit the enzyme activities of rat liver p450s and human cytochrome P450 3A4 (CYP3A4) at physiological levels. Resorufin pentyl ether has also been shown to be toxic in mice, with toxicity studies showing an LD50 of 4.5 mg/kg after oral administration. This drug is not active against bacteria or fungi, but it is able to inhibit protein synthesis in model systems containing mouse monoclonal antibodies or calf thymus DNA.</p>

<p>4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-6-O-benzoyl-3,4-O-isopropylidene a D galactopyranoside is an analog of galactose that binds to the DNA and regulates gene expression. This compound has been shown to be able to regulate gene expression levels in cells. 4NP2B6OB was used as a probe for microarray analysis of gene expression profiles from environmental samples. It was found that 4NP2B6OB could be used as a probe for the identification of genes in high density arrays.</p>

<p>Galβ(1-3)[neu5acα(2-6)]GlcNAc-β-PNP is a glycosylation product of the complex carbohydrate GlcNAc. It is synthesized by the enzyme β-1,3-galactosyltransferase and N-acetylneuraminic acid transferase. This product has been modified using click chemistry to introduce a fluorine atom at the 6 position of Neu5Acα2-6. Galβ(1-3)[neu5acα(2-6)]GlcNAc-β-PNP is available in high purity and custom synthesis.</p>

<p>4-Nitrophenyl 2,3-di-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-benzylidene-b-D-glucopyranoside is a high purity, high quality 4nitrophenol derivative that is used as a food testing substrate. It is also used for the detection of fluorescence in environmental testing. This product can be conjugated with ligands for use in diagnostic assays.</p>

<p>4-Trifluoromethylumbelliferyl Tetra-O-acetylated alpha-D-N-Acetylneuraminate Methyl Ester is a chemiluminescent substrate that is often used in enzyme and immunoassays. It is a high purity, high quality chemical with a CAS number of 195210-09-4. The 4-Trifluoromethylumbelliferyl Tetra-O-acetylated alpha-D-N-Acetylneuraminate Methyl Ester is used in the detection of bacterial infections and can be used to measure the activity of enzymes such as lysozyme and peroxidase. This product has been tested for its ability to induce bioluminescence in bacteria grown in culture media, as well as for its ability to cause fluorescence or staining in cells or tissues. The 4TUMBTM Tetra O Acetylated</p>

<p>6-Chloro-3-indolyl phosphate disodium salt is a substrate for visualising alkaline phosphatase activity. It produces a red colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.</p>

<p>This compound inhibits the enzyme protein kinase. It has been shown to inhibit the activity of a number of protein kinases including those that are involved in cell proliferation, differentiation and apoptosis. This drug is also known to be a potent inhibitor of human c-Jun N-terminal kinase (JNK) and other types of kinases such as cyclin-dependent protein kinases. The inhibition of these enzymes results in the prevention of tumorigenesis, inflammation and immunological responses.</p>

4-Nitrophenyl 6-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that is used in assays for detecting nitrates. This compound reacts with 4-(methylnitrosamino)pyridine (MNAP) to produce an intense light that can be detected by a luminometer. It has been shown to react with the enzyme nitrate reductase and is used in food testing and environmental testing as a means of measuring the amount of nitrates present.

<p>5-Bromo-4-chloro-3-indolyl β-D-glucuronide (BCIG) is an chromogenic enzyme substrate used to detect the presence of glucuronidase enzyme activity. The reaction occurs when BCIG reacts with the enzyme beta-glucuronidase yielding an intense blue color. It is used in a variety of biochemical and biomedical applications, such as the detection of pathogenic bacteria, the diagnosis of genetic disorders, and the isolation of recombinant proteins.</p>

<p>5-Bromo-3-indolyl a-D-mannopyranoside is a chromogenic substrate that is used as an enzyme substrate for various spectrophotometric, fluorometric, or chemiluminescent assays. The 5-bromo group of the dye reacts with the active site of an enzyme to form a product that can be measured by spectrophotometry. This product can also be used as a fluorescent indicator in the presence of luciferase and ATP. 5-Bromo-3-indolyl a-D-mannopyranoside is also used to estimate bacterial counts during culture media preparation and it has been shown to inhibit the growth of Gram negative bacteria such as E. coli and Pseudomonas aeruginosa. This compound does not contain any heavy metals or toxic substances and it is highly soluble in water. It has been found to have no adverse effects on humans and other animals at concentrations up to 100</p>

<p>4-Chloro-3-indoxyl-1,3-diacetate is a chromogenic substrate used in the identification of bile acids. It reacts with 4-chlorophenol to form a red dye. This compound is also used as a ligand for metal ions.</p>

<p>4-Bromo-2-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that reacts with an excess of luciferase to produce light. It is used as a reagent in the detection of ATP, NADH, and other compounds in biochemistry and biology. 4-Bromo-2-nitrophenyl b-D-galactopyranoside is also used in diagnostic kits for detecting bacterial or fungal contamination in food.</p>