Substrats fluorogènes
Dans cette catégorie, vous trouverez une sélection complète de substrats fluorogènes conçus pour la catalyse enzymatique. Ces substrats sont utilisés avec divers types d'enzymes et sont capables de produire de la couleur et de la fluorescence lors de la réaction. Les substrats fluorogènes sont des outils essentiels dans la recherche en biochimie et biologie moléculaire, permettant la détection et la quantification de l'activité enzymatique avec une grande sensibilité et spécificité. Ils sont largement utilisés dans les essais pour étudier la cinétique enzymatique, rechercher des inhibiteurs d'enzymes et surveiller les processus cellulaires. Chez CymitQuimica, nous proposons des substrats fluorogènes de haute qualité pour soutenir vos recherches, garantissant des résultats fiables et précis dans vos essais enzymatiques.
297 produits trouvés pour "Substrats fluorogènes"
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FLsharp™ - Phosphate, disodium salt
FLsharp™ - Phosphate, disodium salt is a fluorogenic water-soluble substrate for phosphatase enzymes. Its chemical name is 6-Chloro-2-[5-chloro-2-(phosphonooxy)phenyl]-4(1H)-quinazolinone disodium salt and was developed by Molecular Probes in the 1990s. After cleavage of the substrate by phosphatase activity, an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissue sections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as a substrate for bacterial phosphatases.
Degré de pureté :Min. 95%4-Methylumbelliferyl beta-D-lactoside
CAS :Fluorogenic substrate for beta-galactosidaseFormule :C22H28O13Degré de pureté :Min. 98 Area-%Couleur et forme :White PowderMasse moléculaire :500.45 g/mol4-Methylumbelliferyl oleate
CAS :Methylumbelliferyl oleate is a MOSCERDAM® substrate targeting the lipase enzymeFormule :C28H40O4Degré de pureté :Min. 90 Area-%Couleur et forme :White PowderMasse moléculaire :440.61 g/mol4-Methylumbelliferyl pyrophosphate diester disodium salt
CAS :4-Methylumbelliferyl pyrophosphate diester disodium salt is a fluorogenic substrate for pyrophosphatases or diphosphatases. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl pyrophosphate diester disodium saltas a substrate for measuring the pyrophosphatases or diphosphatases activity is used for monitoring and diagnosis of pyrophosphatase deficiency and bone metabolism disorder.Formule :C20H14Na2O11P2Degré de pureté :Min. 95%Couleur et forme :White PowderMasse moléculaire :538.25 g/mol4-(Trifluoromethyl)umbelliferyl-β-D-cellotrioside
CAS :4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is a fluorogenic substrate for cellulase. After cleavage, the fluorophore 4-(trifluoromethyl)umbelliferone, commonly referred as TFMU, is released, exhibiting a blueish color. 4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is used to study the enzyme kinetics in bacterial cellulose degradation assays as well as in biomass conversion research studies.Formule :C28H35F3O18Degré de pureté :Min. 95%Couleur et forme :White to off-white solid.Masse moléculaire :716.56 g/mol4-Methylumbelliferyl b-L-fucopyranoside
CAS :A MOSCERDAM® substrate targeting the fucosidase enzymeFormule :C16H18O7Degré de pureté :Min. 98 Area-%Couleur et forme :PowderMasse moléculaire :322.31 g/mol4-Methylumbelliferyl-2-O-(α-L-fucopyranosyl)-β-L-fucopyranoside
4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for diagnosis of fucosidosis (defficiency of L-fucosidase).Degré de pureté :Min. 95%Couleur et forme :White/Off-White SolidMasse moléculaire :468.45 g/molResorufin b-D-xylobioside
Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.Degré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :477.42 g/mol4-Methylumbelliferyl dodecanoate
CAS :4-Methylumbelliferyl dodecanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl dodecanoateas a substrate for measuring the lipase activity is used for detecting pancreatic insufficiency in diagnosis assays as well as the screening of lipase deficiency.Formule :C22H30O4Degré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :358.47 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid 2-sulphate disodium salt - Moscerdam™ biochemical purity
CAS :PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.Formule :C16H14Na2O12SDegré de pureté :Min. 99 Area-%Couleur et forme :White PowderMasse moléculaire :476.32 g/mol4-Methylumbelliferyl a-D-glucosaminide
CAS :4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.Formule :C16H19NO7Degré de pureté :Min. 90 Area-%Couleur et forme :White PowderMasse moléculaire :337.32 g/molL-Alanine-7-amido-4-methylcoumarin hydrochloride
CAS :L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.Formule :C13H14N2O3·HClDegré de pureté :Min. 90%Couleur et forme :PowderMasse moléculaire :282.72 g/mol4-Methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside
CAS :4-Methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside is a fluorogenic substrate for alpha-N-acetylgalactosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranosideas a substrate for measuring the alpha-N-acetylgalactosaminidase activity is used for Fabry disease diagnosis and Schindler disease screening.Formule :C24H31NO13Degré de pureté :Min. 95%Couleur et forme :White PowderMasse moléculaire :541.5 g/mol(+)-Biotin 4-amidobenzoic acid sodium salt
CAS :(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.
Formule :C17H20N3NaO4SDegré de pureté :Min. 95%Couleur et forme :White Slightly Yellow PowderMasse moléculaire :385.41 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid sodium salt
CAS :PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI179383.
4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).Formule :C16H15NaO9Degré de pureté :Min. 98 Area-%Couleur et forme :White PowderMasse moléculaire :374.27 g/mol4-Methylumbelliferyl palmitate
CAS :4-Methylumbelliferyl palmitate, commonly names as 4-MUP, is a fluorescent probe that binds to lipases, enzymes responsible to assist on the hydrolysis of fats. In diagnosis, 4-MUP is used for screening of lysosomal storage diseases (LSD) caused by a deficiency of a single enzyme involved in the metabolism of glycoproteins, lipids or mucopolysaccharides.
Formule :C26H38O4Degré de pureté :Min. 95%Couleur et forme :White PowderMasse moléculaire :414.58 g/molResorufin b-D-glucuronide sodium salt
CAS :Resorufin b-D-glucuronide sodium salt is used as a fluorogenic substrate for beta-glucuronidase (GUS) to study drug metabolism.Degré de pureté :Min. 95%Couleur et forme :Brown Orange Red PowderMasse moléculaire :411.29 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside-4-sulfate sodium salt
4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-4-sulfate sodium salt is a fluorogenic substrate for N-acetylgalactosamine-4-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-4-sulfate sodium saltas a substrate for measuring the N-acetylgalactosamine-4-sulfatase activity is used for diagnosis of Mucopolysaccharidosis VI (MPS VI) (a rare lysosomal storage disorder) and Maroteaux-Lamy syndrome screening.Degré de pureté :Min. 95%Couleur et forme :White PowderMasse moléculaire :481.41 g/mol6-Hexadecanoylamino-4-methylumbelliferone
CAS :Fluorochrome for calibration of fluorimeters. Used with corresponding fluorogenic substrates for the diagnosis of Krabbe - and Niemann-Pick A/B disease.Formule :C26H39NO4Degré de pureté :Min. 95%Couleur et forme :White PowderMasse moléculaire :429.59 g/molFluorescein mono-b-D-galactopyranoside
CAS :Fluorescein mono-b-D-galactopyranoside or FMG is a fluorogenic substrate for beta-galactosidase. The fluorophore fluorescein is released after enzymatic cleavage, exhibiting a greenish fluorescent. This substrate is particularly valuable in gene expression studies (e.g., lacZ reporter assays) and as a probe for cell-based assays for real-time monitoring.Degré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :494.45 g/mol
