Substrats fluorogènes

<p>Resorufin benzyl ether is a chemical compound that can be used as a substrate for cytochrome P450. It has been shown to inhibit the activity of epoxide hydrolase, an enzyme involved in the metabolism of endoperoxides. Resorufin benzyl ether has also been found to inhibit cytochrome P450 enzymes, which are responsible for oxidative metabolism of xenobiotics. This compound has been shown to have a high affinity for mouse monoclonal antibody and is used in immunoassays to detect mouse antibodies. Resorufin benzyl ether is used in biochemical assays and polymerase chain reactions (PCRs) as a fluorescence marker. In addition, this compound is used in model systems to study enzyme activities such as recombinant cytochrome p450 and protein oxidation by reactive oxygen species (ROS).</p>

<p>4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. It can be used in diagnostic tests to identify antibodies against certain bacteria and fungi. The fluorogenic form of 4-methylumbelliferyl 2-acetamido-3,4,6-triO acetyl -2 deoxy a D glucopyranoside can be used as a chemiluminescent substrate for detecting bacterial activity. 4 methylumbelliferyl 2 acetamido 3,4,6 triO acetyl -2 deoxy a D glucopyranoside is also useful in bioluminescent assays to detect ATP production in bacteria and other microorganisms. CAS No: 1174287–76–3</p>

<p>4-Methylumbelliferyl 2-acetamido-3-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-4-O-(2,3,4,6 -tetra-O-acetylbDgalactopyranosyl)-6-O-acetyldEoxybDglucopyranoside is a chemiluminescent substrate that has been used in the detection of beta galactosidase activity. It is a ligand for beta galactosidases and can be used for the study of enzyme kinetics. This compound also exhibits high purity and is available in CAS number: 916071. 4MUFAGAALF is suitable for use in culture media and environmental testing as well as diagnostics (e.g., bioluminescence), staining (fluorogenic substrate), and chromogenic substrates</p>

<p>4-Methylumbelliferyl 2-azido-2-deoxy-3-O-(tetra-O-acetyl-b-D-galactopyranosyl)-4,6-O-benzylidene a D galactopyranoside is a chemiluminescent substrate for detecting ligands. It has been used to detect the presence of bacteria, fungi, and parasites in food samples and as an indicator for enzymatic activity. This product is an excellent fluorescent substrate for the detection of nucleic acids such as DNA. This product is a conjugate with high purity, which makes it suitable for use in various types of applications.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-3-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-3-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis (deficiency of the enzyme alpha-L-fucosidase) and lysosomal storage disorder screening.</p>

<p>6-Chloro-4-trifluoromethylumbelliferyl b-D-galactopyranoside is a chromogenic substrate that can be used in enzyme assays to measure the activity of beta-glucuronidase. This compound is sold as a conjugate with an enzyme, which will not interfere with the assay. The compound has been tested to have high purity and quality.</p>

<p>Resorufin maltotriose is an alpha-amylase substrate that releases the dye resorufin with red emission (lmax=585 nm) after enzyme reaction.</p>

<p>Caspase substrates are a class of pharmacological agents that regulate apoptosis. Caspases are proteases that cleave specific proteins in the cell, leading to apoptosis. Pro-apoptotic proteins activate caspases, while anti-apoptotic proteins inhibit them. Caspase substrates can be either pro-caspase or anti-caspase, depending on the desired effect. They may also be used to regulate caspases without affecting their activity. The caspase substrate is cleaved by caspases and releases a polypeptide fragment that triggers apoptosis in some cells, but not others. For example, the Hl-60 cell line is resistant to the pro-apoptotic effects of the substrate because it contains an anti-apoptotic protein called Bcl-2. In contrast, myocardial cells contain pro-apoptotic proteins and are sensitive to the pro-apoptotic effects of the substrate. In</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-3,6-di-O-pivaloyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with an enzyme to produce light. It is used in the detection of ATPase activity and in the diagnosis of diseases. This product has been tested for quality and environmental safety, and it does not contain any hazardous substances. It is highly pure and can be used for testing food, diagnosing diseases, or staining cells.</p>

<p>4-Trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid is a fluorescent chemiluminescent substrate that is used in the detection of beta-galactosidase activity. Beta-galactosidase catalyzes the hydrolysis of 4-trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid to form 4-trifluoromethylumbelliferone and D-galactose, which emits light at approximately 480 nm. This reaction can be detected by fluorescence spectrophotometry using a fluorometer or photometer. 4TFUNA is not toxic, does not inhibit enzymes, and has been tested for toxicity and mutagenicity using bacteria and mammalian cells.</p>

<p>6,8-Difluoro-4-methylumbelliferyl b-D-cellotrioside is a ligand that binds to active sites of enzymes such as beta-glucuronidase and beta-glucosidase. It is used in diagnostics for the detection of bacteria in culture media and food testing where it can be used as a chromogenic or fluorogenic substrate. 6,8-Difluoro-4-methylumbelliferyl b-D-cellotrioside is also used to detect the presence of bacteria and fungi in environmental samples. It has a high purity and conjugate with other molecules for use in fluorescence microscopy.</p>

<p>4-Methylumbelliferyl 2,3-di-O-benzoyl-4,6-O-benzylidene-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of β-galactosidase activity. This product is a white to off white powder that has a purity of 99.2%. It has been given CAS number 53654–95–1 and it can be found in the Diagnostics section of Acros Organics.</p>

<p>4-Methylumbelliferyl 4-deoxy-b-D-chitobiose peracetate is a chromogenic substrate used for the detection of β-galactosidase activity. It is an analog of 4-methylumbelliferone that can be hydrolyzed by β-galactosidase to produce fluorescence. This product is supplied as a white crystalline solid and can be stored at room temperature. This substrate has been shown to work well in culture media, food testing, and environmental testing.</p>

<p>5-Octanoylaminofluoroscein di-b-D-glactopyranoside is a chemiluminescent substrate that can be used for the detection of peroxidase activity. It is a bifunctional substrate, which means it has two reactive groups: one at the 5 position and one at the 8 position. The 5-octanoylaminofluorescein reacts with hydrogen peroxide in a 1:1 ratio, emitting light at 490 nm. The 8-position amine reacts with 2,2'-azino-bis(3-ethylbenzthiazoline)6 sulphonic acid (ABTS) to produce an orange color product that acts as a chromogen. The substrate can be used in diagnostics, food testing, and enzyme substrates.</p>

<p>5-Acetamidofluorescein-di-(b-D-galactopyranoside), also known as C2FDG, is a fluorogenic substrate for beta-galactosidase that generates a green fluorescence after cleavage (Em ~520 nm). It is used in enzyme activity assays and in flow cytometry in animal, bacterial, and yeast cells.</p>

<p>Fluorogenic substrate for beta-glucuronidase</p>

<p>6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-galactopyranoside is a chromogenic substrate for the enzyme β-galactosidase, which hydrolyzes the substrate to produce a fluorescent product. This fluorogenic substrate can be used in chemiluminescent detection of β-galactosidase activity as well as in immunochemical and immunohistochemical analysis of glycoproteins. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-galactopyranoside is also used for staining of glycoprotein substrates with periodic acid Schiff reagent or Alcian blue, and it can be used in the diagnosis of glycogen storage diseases.</p>

<p>6'-(O-(Carboxymethyl))-2',7'-dichlorofluoroscein 3'-(O-(N-acetyl-b-D-glucosamide)) is a chemiluminescent substrate that can be used in the detection of ligands. This product has a CAS number of 44636-76-8 and is available at a purity level of 98%. It has been tested for bacterial contamination, as well as for adverse effects on culture media and environmental testing. 6'-(O-(Carboxymethyl))-2',7'-dichlorofluoroscein 3'-(O-(N-acetyl-b-D-glucosamide)) is an enzymatic substrate that can be used in diagnostic applications such as the detection of fluorescence, staining, and bioluminescence. It has also been shown to be useful in food testing.</p>

<p>6-Chloro-4-methylumbelliferyl b-D glucuronide is a fluorogenic substrate for beta-glucuronidase that exhibits longer absorption/emission maxima (~385/500 nm) than typical 4MU-derived substrates.</p>

<p>4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraoside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraosideas a substrate for measuring the chitinase activity is used for fungal infection detection and Gaucher disease screening.</p>