Substrats fluorogènes

For fluorogenic detection of caspase 3

5-Octanoylaminofluoroscein di-b-D-glactopyranoside is a chemiluminescent substrate that can be used for the detection of peroxidase activity. It is a bifunctional substrate, which means it has two reactive groups: one at the 5 position and one at the 8 position. The 5-octanoylaminofluorescein reacts with hydrogen peroxide in a 1:1 ratio, emitting light at 490 nm. The 8-position amine reacts with 2,2'-azino-bis(3-ethylbenzthiazoline)6 sulphonic acid (ABTS) to produce an orange color product that acts as a chromogen. The substrate can be used in diagnostics, food testing, and enzyme substrates.

4-Methylumbelliferyl 2,3,4-tri-O-benzoyl-b-D-galactopyranoside-6-sulfate is a fluorogenic substrate for β-galactosidase and a chromogenic substrate for β-glucuronidase. It is used in food testing, environmental testing, and diagnostics.

6,8-Difluoro-4-methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside is a chemiluminescent substrate that reacts with oxygen in the presence of luciferase to produce light. This product is an excellent replacement for luminol, especially in applications where aqueous solvents are not suitable. 6,8-Difluoro-4-methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside has been extensively tested and shown to be nonhazardous in a variety of different culture media. It is also nonhazardous to the environment, as it can be easily degraded by microorganisms.

Fluorogenic substrate targeting caspases 3 and 7

Caspase substrates are a class of pharmacological agents that regulate apoptosis. Caspases are proteases that cleave specific proteins in the cell, leading to apoptosis. Pro-apoptotic proteins activate caspases, while anti-apoptotic proteins inhibit them. Caspase substrates can be either pro-caspase or anti-caspase, depending on the desired effect. They may also be used to regulate caspases without affecting their activity. The caspase substrate is cleaved by caspases and releases a polypeptide fragment that triggers apoptosis in some cells, but not others. For example, the Hl-60 cell line is resistant to the pro-apoptotic effects of the substrate because it contains an anti-apoptotic protein called Bcl-2. In contrast, myocardial cells contain pro-apoptotic proteins and are sensitive to the pro-apoptotic effects of the substrate. In

Fluorogenic substrate used to differentiate cytochrome P450 monooxygenases

4-Methylumbelliferyl 2,3-di-O-benzoyl-4,6-O-benzylidene-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of β-galactosidase activity. This product is a white to off white powder that has a purity of 99.2%. It has been given CAS number 53654–95–1 and it can be found in the Diagnostics section of Acros Organics.

This Clips-O substrate 7-(4'-L-Alaninamido)-rac-(3'-octanoyloxy)butyloxy) coumarin allows the detection of three enzymatic activities (L-alanine aminopeptidase, C8-esterase and oxidation of 4-amino-3-hydroxybutyloxy either enzymatically or by exposure to sodium periodate) with one single substrate and give a positive fluorescent signal only in presence of all activities. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.