Électrophorèse
Dans cette section, vous trouverez tous les réactifs et matériaux nécessaires pour réaliser l'électrophorèse, une technique de laboratoire essentielle utilisée pour séparer les protéines en fonction de leur masse moléculaire et de leur charge. Cette technique est cruciale pour analyser la composition et la pureté des échantillons de protéines, ainsi que pour étudier les acides nucléiques et autres biomolécules. Notre sélection comprend des tampons d'électrophorèse, des gels, des colorants et des marqueurs de haute qualité, ainsi que des appareils et accessoires conçus pour garantir une séparation précise et efficace. Ces produits sont indispensables pour la recherche en biologie moléculaire, biochimie et génétique, fournissant des résultats fiables pour la caractérisation des protéines, l'analyse de l'expression génique et d'autres applications. Chez CymitQuimica, nous fournissons tout ce dont vous avez besoin pour réaliser l'électrophorèse, soutenant vos recherches avec précision et cohérence.
Sous-catégories appartenant à la catégorie "Électrophorèse"
298 produits trouvés pour "Électrophorèse"
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N,N'-Methylene Bisacrylamide 2% Aq. Solution for molecular biology
CAS :Formule :C7H10N2O2Couleur et forme :Clear, Colourless, liquidMasse moléculaire :154.171X Phosphate Buffered Saline (PBS) for molecular biology
The pH of the buffer is typically maintained within a range of 7.4 ± 0.1, closely resembling the physiological pH found in many biological systems.Couleur et forme :Clear, Colourless, Liquid10X Phosphate Buffered Saline Tween-20 (PBST) for molecular biology
Couleur et forme :Clear, Colourless with yellow tinted, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-7.2 for molecular biology
Couleur et forme :Clear, Colourless, LiquidBrilliant Blue R-250 for molecular biology
CAS :Formule :C45H44N3NaO7S2Couleur et forme :Dark blue with reddish tinge, PowderMasse moléculaire :825.9810X MOPS Buffer for molecular biology
MOPS, or morpholino propanesulfonic acid, is a structural analog of MES, the ethanesulfonic acid. With a pKa value of 6.80-7.20, MOPS serves as an excellent buffer for many biological systems at near-neutral pH. It is extensively used as a buffering agent in molecular biology and biochemistry and has been tested and recommended for use in polyacrylamide gel electrophoresis. Additionally, MOPS is applicable in various bioanalytical methods, including isoelectric focusing, protein assays, and X-ray crystallographic studies. Furthermore, it can be used as an electrophoresis buffer for agarose gel electrophoresis of RNA.Couleur et forme :Clear, Colourless, Liquid20X SSC Buffer pH 6.9-7.1 for molecular biology
The Saline-Sodium Citrate Hybridization Buffer [20X] is a key component in various nucleic acid techniques, ensuring optimal stringency during washing steps in procedures such as Southern blotting, in situ hybridization, DNA microarrays, and Northern blotting. When utilized as 20X SSC, it effectively prevents agarose gels from drying out during vacuum transfers, preserving sample integrity. This solution is specifically formulated to support the diverse needs of end users in nucleic acid hybridizations and blot transfer applications, making it an invaluable resource in molecular biology workflows.Couleur et forme :Clear, Colourless, SolutionLithium Lauryl Sulphate extrapure, 99%
CAS :Formule :C12H25SO4LiDegré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :272.30Albumin Bovine Solution 10% Diluent Solution in PBS (BSA 10% Diluent/Blocking Solution in PBS)
CAS :Couleur et forme :Clear, Pale yellow, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-8.0 for molecular biology
Couleur et forme :Clear, Colourless, Liquid20X SSPE Buffer pH-7.4 suitable for molecular biology
The 20X SSC Buffer, with a pH between 6.9 and 7.1, is essential for in molecular biology, particularly for Southern and Northern hybridization techniques. This solution is sterilized through a 0.22 µm filter, making it suitable for direct use or for dilution based on experimental requirements. In parallel, 20X SSPE is available as a concentrated buffer intended for nucleic acid hybridizations and blot transfer processes. A significant feature of SSPE is its inclusion of EDTA, which chelates divalent metal ions like Mg²+. This property effectively inhibits DNase activity, helping to preserve the concentrations of probe and target DNA during Southern hybridization, thereby enhancing the accuracy of the results.Couleur et forme :Clear, Colourless, Liquid10X Tris-Tricine-SDS Buffer for molecular biology
The Tris-Tricine-SDS Gel Running Buffer is specifically designed for separating proteins with molecular weights between 1 and 100 kDa, making it particularly effective for resolving proteins smaller than 30 kDa. This approach differs from traditional SDS-PAGE by substituting glycine (pK 9.6) with tricine (pK 8.15), which enhances the stacking and destacking of low molecular weight proteins and improves the resolution of smaller peptides due to the differing pK values. Incorporating urea into the stacking gel allows for the effective separation of two proteins that share the same molecular weight. Additionally, the lower acrylamide concentrations in Tricine gels facilitate the transfer of hydrophobic proteins during Western blotting. Tricine–SDS-PAGE is therefore commonly employed for the separation of lower molecular weight proteins.Couleur et forme :Clear, Colourless, Liquid
