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<p>2-(N-Hexadecanoylamino)-4-nitrophenylphosphocholine hydroxide, often abbreviated as HDA-PC, is a chromogenic substrate for acid sphingomyelinase enzyme. HDA-PC is used as a key substrate for measuring the activity of this enzyme, responsible for catalyzing the hydrolysis of sphingomyelin to ceramide. The activity assay for this measuring this enzyme's acitivy is to detect a deficiency (or lack) of the enzyme which is directly related to the Niemann-Pick disease.</p>

<p>5-Bromo-4-chloro-3-indoxyl butyrate is a colorimetric substrate used to detect and quantify esterase activity. Upon hydrolysis by esterases, it yields a blue-green dye, allowing the detection and quantification of enzyme activity. It is commonly used in assays for the screening of esterase-producing microorganisms and in research aimed at understanding the role of esterases in various cellular processes.</p>

<p>6-Chloro-3-indoxyl palmitate is a high quality, pure chemical reagent that can be used as a substrate for enzyme activity. It is a chromogenic substrate that is used in the detection of esterase activity. 6-Chloro-3-indoxyl palmitate is also used as a fluorogenic substrate in the presence of horseradish peroxidase and hydrogen peroxide to produce chemiluminescence. 6-Chloro-3-indoxyl palmitate has been shown to be an effective bioluminescent substrate for luciferase enzymes, such as firefly luciferase and Renilla luciferase.</p>

<p>Aldol 495 acetate is a chromogenic substrate that contains the chemical compound Aldol. It is used in food testing, diagnostics, and environmental testing. Aldol 495 acetate can be used as a ligand to detect the presence of enzymes or bacteria in culture media and diagnostics. This product has been shown to have a high purity and quality.</p>

<p>Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>2-Naphthyl-β-D-galactopyranoside is a substrate for β-galactosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.</p>

<p>5-Bromo-6-chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in mucopolysaccharidosis screening.</p>

<p>Histochemical substrate for alkaline phosphatase</p>

<p>4-Nitrophenyl b-D-thioglucopyranoside is a highly sensitive chromogenic substrate designed for the colorimetric detection and quantification of thioglucosidase enzymes. Upon enzymatic cleavage, it releases a highly absorbing 4-nitrophenyl moiety, generating a yellow color that can be readily measured spectrophotometrically. This substrate is widely utilized in the study of enzyme kinetics, substrate specificity, and inhibitor screening for thioglucosidases involved in various metabolic pathways and biological processes.</p>

<p>Aldol® 518 beta-D-glucuronic acid is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucuronidase activity, yielding red colour and red fluorescence. Living cells of Escherichia coli can be reliably detected with Aldol® 518 beta-D-glucuronic acid-containing media. Aldol® 518 beta-D-glucuronic acid can be also used in GUS reporter assays, e.g. in plant biotechnology. Aldol® 518 beta-D-glucuronic acid is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.</p>

<p>5-Bromo-6-chloro-3-indolyl acetate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in bacterial esterase detection (e.g. Staphylococcus spp.).</p>

<p>3-Indoxyl caprylate is an enzyme substrate used in a variety of diagnostic applications, including the detection of various bacterial and viral infections. It is used as a substrate for beta-galactosidase, which can be detected by bioluminescence or chromogenic substrates. 3-Indoxyl caprylate also serves as a ligand for receptor binding and has been shown to bind to CD4 receptors on human T cells. 3-Indoxyl caprylate is also used as an enzyme substrate in the detection of DNA polymerases, RNA polymerases, and ATPases. This product is available with a high purity that ranges from 98% to 99%. 3-Indoxyl caprylate is soluble in water, alcohols, acetone, acetic acid, and chloroform.</p>

<p>Chromogenic substrate for β-D-galactosidase, yielding a blue precipitate upon cleavage.</p>

<p>Chromogenic substrate for phosphatase</p>

<p>6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.</p>

<p>4-Nitrophenyl b-D-thiogalactopyranoside is a specialized chromogenic substrate designed for measuring the activity of thio-β-galactosidase enzymes. Upon cleavage by thio-β-galactosidase, it releases the intensely colored 4-nitrophenolate ion, providing a straightforward, sensitive, and quantitative detection method. This substrate is easily incorporated into enzyme-coupled assays, making it a reliable and convenient choice for research and diagnostic applications. With its rapid color development and high signal-to-background ratio, 4-Nitrophenyl b-D-thiogalactopyranoside is ideal for monitoring enzyme kinetics, substrate specificity evaluation, and inhibitor screening in a range of scientific and biotechnological settings.</p>

<p>6-Bromo-2-naphthyl alpha-D-glucopyranoside is a chromogenic substrate used to detect alpha-glucosidase enzyme activity. When cleaved by the enzyme alpha-glucosidase, it releases 6-bromo-2-naphthol, which can be coupled with diazonium salts (for example 4'-amino-2,3'-dimethylazobenzene, also known as Fast Garnet GBC) to form a colored precipitate. 6-Bromo-2-naphthyl alpha-D-glucopyranoside is used in biochemical assays, histochemistry, and diagnostic tests for conditions like lysosomal storage disorders (e.g. Pompe disease) and carbohydrate metabolism studies.</p>

<p>3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.</p>

<p>Chromogenic substrate for esterase with C8 activity yielding a blue precipitate upon cleavage.</p>

<p>Chromogenic substrate for aryl-sulfatase. Yields a blue precipitate upon cleavage.</p>

<p>6-Chloro-3-indoxyl caprylate is a high quality, enzyme substrate, chromogenic substrate, bioluminescence, chemiluminescence ligand, staining and diagnostics reagent. It has CAS No. 159954-35-5 and is used in food testing, culture media and environmental testing. 6-Chloro-3-indoxyl caprylate is also a fluorogenic substrate that is used for high purity applications such as conjugation with other molecules.</p>

<p>Chromogenic substrate to visualize the activity of alpha-D-mannopyranoside; red color produced</p>

<p>2-Nitrophenyl b-D-cellobioside heptaacetate is a chromogenic enzyme substrate used to assay for cellobiohydrolase activity. The substrate undergoes enzymatic hydrolysis, releasing 2-nitrophenol which can then be detected via absorbance at 400 nm. This type of substrate is also used for the analysis of cellulose and hemicelluloses in plant materials. The heptaacetate modification enhances solubility, making it ideal for use in organic solvents, and it also protects against unwanted hydrolysis by acid or alkali. The substrate can be used in a variety of applications, including enzyme assay development, screening for inhibitors or activators, and in the study of the mode of action and substrate specificity of cellulase enzymes.</p>

<p>Naphthol AS-BI N-acetyl-beta-D-glucosaminide is a fluorogenic substrate for the pararosaniline-glyoxalase (PG) reaction. This substrate is used to study enzymatic activity in two human isoenzymes of PG, PG I and PG II. The conversion of this substrate by PG I or PG II results in a color change from yellow to red. Naphthol AS-BI N-acetyl-beta-D-glucosaminide may be used as an experimental tool to measure the activity of these enzymes and to study the effect of inhibitors on their activity. It can also be used as a marker for studying enzyme transfer between cells.</p>

<p>N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.</p>

<p>4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester, also known as 4-bromo-4'-(N,N-dimethylamino)biphenyl, is a bioluminescent substrate. It is used for the detection of beta-glucuronidase activity in biological samples and for the detection of bacterial β-glucuronidase activity in culture media. Beta-glucuronidase catalyzes the hydrolysis of glucuronic acid by breaking down a conjugate molecule into two smaller molecules, which can be detected using bioluminescence. 4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is a chromogenic substrate that has been shown to be an excellent ligand in biomolecular research because it reacts with many different enzymes. This product is highly pure and</p>

<p>Aldol 584 is a chromogenic substrate that is used in enzymatic assays to detect the presence of an enzyme. It is a triethylammonium salt with beta-D-glucuronic acid. Aldol 584 is used in diagnostic procedures and can be used as a substrate for fluorogenic substrates and chemiluminescent substrates. This product has been shown to be effective in detecting the presence of bile in feces, erythrocyte hemolysis, and the presence of proteinases in human urine. It can also be used as a food testing agent or to measure bacterial activity in culture media.</p>

<p>Aldol® 518 myo-inositol phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13) activity. The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms grown in liquid or solid media, yielding intense red color. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also be obtained. Aldol® 518 myo-inositol phosphate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus species. Aldol® 518 myo-inositol phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>1-Naphthyl a-D-galactopyranoside is a chromogenic substrate use to detect alpha-galactosidase activity.</p>

<p>6-Bromo-2-naphthyl b-D-glucopyranoside is an enzyme substrate used in chromogenic assays with beta-glucosidases. It has been used in culture media for the identification of Legionella pneumophila.</p>

<p>3-Nitrophenyl beta-D-glucopyranoside is a chromogenic enzyme substrate commonly used to assay for beta-glucosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be easily detected by spectrophotometry. This substrate is often used in the field of carbohydrate chemistry and biochemistry to study enzyme kinetics and inhibition in both in vitro and in vivo systems.</p>

<p>2-Naphthyl b-D-mannopyranoside is a chromogenic substrate specifically designed for the detection and quantification of β-mannosidase enzyme activity. Upon hydrolysis by β-mannosidase, this substrate releases a colored product, 2-naphthol, which can be easily monitored spectrophotometrically. The use of 2-Naphthyl b-D-mannopyranoside allows for the rapid and sensitive measurement of β-mannosidase activity in various applications, such as enzyme assays, screening for β-mannosidase-producing microorganisms, and studying the kinetics and inhibition of β-mannosidase enzymes.</p>

<p>5-Bromo-3-indolyl-beta-D-galactopyranoside-6-sulfate sodium salt is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in sulfatase activity assays and for the diagnosis of Mucopolysaccharidosis IVA (Morquio A).</p>

<p>4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is a synthetic substrate that is used in enzyme kinetics. It is a beta-galactosidase substrate that reacts with the enzyme to form a product. The rate of reaction can be measured by following the change in absorbance over time. This product will react with an acceptor, such as chondroitin sulfate, to produce an observable reaction product. 4NPBGA has been shown to be an effective carbon source for plant physiology studies and has been shown to have optimal activity at pH 6.5.</p>

<p>2-Naphthyl-lpha-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.</p>

<p>Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for alpha-glucosidase activity (EC 3.2.1.20, alpha-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>6-Chloro-3-indolyl butyrate is a chromogenic substrate for lipases. Use in lipid digestion and metabolism studies, it produces a salmon-pink color when 6-chloro-3-indolyl is released after enzymatic cleavage.</p>

<p>p-Nitrophenylphosphorylcholine is a substrate molecule for enzymes that catalyze phospholipid biosynthesis. It is the most common substrate used to study the enzyme activity of phosphatidylcholine synthase and phospholipase A2 in human serum. The reaction mechanism is not well understood, but it appears to involve autocatalysis and an initial nucleophilic attack by p-nitrophenolate on the carbonyl carbon atom of the choline moiety. p-Nitrophenylphosphorylcholine has been shown to exhibit receptor activity on cells, which may be due to its ability to bind with basic proteins. This compound can also inhibit meiosis in Drosophila melanogaster and increase levels of natriuretic peptides in rats, as well as cause cell lysis and polymerase chain reactions in mammalian cells.</p>

<p>1-Naphthyl beta-D-glucuronide (NPG) is a chromogenic enzyme substrate used to detect the presence of β-glucuronidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is commonly used in clinical assays to detect the presence of the enzyme in urine samples, as elevated levels can indicate liver or urinary tract diseases. It is also utilized in environmental testing, as some bacteria strains can produce the enzyme as a by-product, making NPG a useful tool for detecting these strains in contaminated water or food sources.</p>

<p>Chromogenic substrate for beta-glucuronidase</p>

<p>5-Bromo-6-chloro-3-indoxyl butyrate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lipid digestion assays.</p>

<p>Aldol® 515 palmitate is a chromogenic substrate that is used in food testing, diagnostics, and other applications. It has a high purity and is conjugated to various ligands for specific applications. Aldol® 515 palmitate is an excellent chemiluminescent substrate for the detection of ATP and other nucleotides. It also has high bioluminescence activity for the detection of adenosine-5'-triphosphate (ATP) or luciferin as well as enzyme substrates to measure β-galactosidase or β-glucuronidase activity.</p>

<p>3-Indoxyl phosphate p-toluidine salt is a secretor of blood group A. It is used in the detection of the presence of alkaline phosphatase in serum and other body fluids. This compound has been shown to be an isoform of 3-indoxyl phosphate with a molecular mass of about 4,000 daltons. It is also used as a substrate for gel electrophoresis and filtration experiments. The purified form of this compound has been shown to have an alkaline phosphatase activity that is ˜4 times greater than that found in human serum and intestinal extracts.</p>

<p>Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>2-Naphthyl caprylate is a substrate that is employed in an assays for carboxylesterase and lipase and when hydrolyzed, produces 2-naphthol, a compound that can also be identified via fluorescence analysis. By combining it with a diazonium salt, the formation of the corresponding azo dye occurs. In particular, this substrate is used to detect the extracellular lipase of Pseudomonas fluorescens B52.</p>

<p>Chromogenic substrate for beta-glucanase</p>

<p>Indoxyl substrate targeting the enzyme inositol monophosphatase; Magenta color</p>

<p>6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a pink enzyme substrate commonly used in biochemical research and diagnostic applications. This compound is a derivative of indolyl glucopyranoside, which is known for its ability to produce a colored product upon enzymatic hydrolysis. 6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is particularly useful for studying glycosidases, enzymes that cleave glycosidic bonds in complex carbohydrates. Its pink coloration makes it an ideal choice for colorimetric assays, enabling researchers to monitor enzyme activity in real-time and facilitating the development of new diagnostic tools and therapeutic strategies.</p>