Substrats d'enzymes
Les substrats enzymatiques sont des molécules sur lesquelles les enzymes agissent pour catalyser des réactions chimiques. Le substrat se lie au site actif de l'enzyme, où il subit une transformation spécifique pour former le produit de la réaction. Les substrats enzymatiques sont des outils essentiels dans la recherche pour étudier la cinétique enzymatique, la spécificité et le mécanisme d'action. Ils sont également utilisés dans les essais diagnostiques et les études biochimiques. Chez CymitQuimica, nous offrons une sélection diversifiée de substrats enzymatiques de haute qualité pour soutenir vos recherches en enzymologie et biochimie.
Sous-catégories appartenant à la catégorie "Substrats d'enzymes"
1527 produits trouvés pour "Substrats d'enzymes"
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4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside
CAS :4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside is a molecule that has been expressed in the yeast Pichia pastoris. It can be used as a fluorescent probe for chitinase activity and has been shown to be sensitive to tryptophan residues and aromatic residues in proteins. 4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside has also been used as a fluorescent probe for isozymes of glucose phosphate isomerase. The nature of the trisaccharide is unknown, but it appears to be hydrophobic.Formule :C26H34N2O13Degré de pureté :Min. 99.0 Area-%Masse moléculaire :582.57 g/mol4-Methylumbelliferyl-beta-D-mannopyranoside
CAS :Fluorogenic substrate for detection of β-D-mannosidase.Formule :C16H18O8Degré de pureté :Min. 99.0 Area-%Masse moléculaire :338.31 g/mol5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside
CAS :5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-mannosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is used in alpha-mannosidosis diagnosis and other lysosomal storage disorder.Formule :C14H15BrClNO6Degré de pureté :Min. 95 Area-%Couleur et forme :PowderMasse moléculaire :408.63 g/mol4-Methylumbelliferyl lignocerate
CAS :This botanical is a download for the Floral Database.
Formule :C34H54O4Degré de pureté :Min. 95 Area-%Masse moléculaire :526.81 g/molRef: 3D-M-5720
1gÀ demander5gÀ demander250mgÀ demander500mgÀ demander2500mgÀ demander-Unit-ggÀ demander5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt
5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.Degré de pureté :Min. 95%Resorufin β-D-cellobioside
CAS :Resorufin beta-D-cellobioside is a fluorogenic substrate for the enzyme beta-glucosidase. It is soluble in water and can be used as a chromogenic substrate for beta-galactosidase. Resorufin beta-D-cellobioside is also used as a ligand to study protein interactions, such as those between lectins and glycoproteins. This product is of high purity and quality, with CAS No. 1000404-48-7.Formule :C24H27NO13Degré de pureté :Min. 95 Area-%Masse moléculaire :537.47 g/molRef: 3D-R-2056
10mgÀ demander25mgÀ demander50mgÀ demander100mgÀ demander250mgÀ demander-Unit-ggÀ demanderAldol® 458 phosphate, disodium salt, Biosynth Patent: EP 2427431 and US 8940909
CAS :Aldol® 458 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by phosphatases secreted by bacteria grown in liquid media, yielding yellow color and green fluorescence. Aldol® 458 phosphate can also be used in assays involving phosphatase-coupled antibodies in immunoassay applications (ELISA). Aldol® 458 phosphate can be used under aerobic and anaerobic conditions.Formule :C16H12NNa2O6PDegré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :391.22 g/mol4-Methylumbelliferyl b-D-cellopentoside
CAS :A fluorogenic MOSCERDAM® substrate targeting cellulasesFormule :C40H58O28Degré de pureté :Min. 95%Couleur et forme :White To Off-White SolidMasse moléculaire :986.89 g/molGlycyl-glycine 7-amido-4-methylcoumarin hydrochloride
CAS :Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride (Gly-Gly-AMC) is a fluorescent compound that can be used as a probe for the transfer of glycine in the brain. Gly-Gly-AMC binds to Langerhans cells and is transferred by an anterograde axonal transport mechanism from the peripheral nervous system to the central nervous system. The binding of Gly-Gly-AMC to Langerhans cells has been shown to activate them, which induces the release of inflammatory mediators into the surrounding tissue. This activity may be due to increased levels of calcium ions, which are known to induce exocytosis in these cells. Glycine is also involved in depression and other mental disorders, as well as muscle and brain activity.Formule :C14H16ClN3O4Masse moléculaire :325.75 g/molRef: 3D-G-6100
1gÀ demander5gÀ demander10gÀ demander500mgÀ demander2500mgÀ demander-Unit-ggÀ demander4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside
CAS :4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.Formule :C28H41N3O18Degré de pureté :Min. 95 Area-%Couleur et forme :White PowderMasse moléculaire :707.63 g/molAldol® 515 choline phosphate, Biosynth Patent: EP 2427431 and US 8940909
A useful substrate for the study of enzyme activitiesFormule :C28H32N3O5PDegré de pureté :Min. 95 Area-%Masse moléculaire :521.55 g/mol4-Nitrophenyl-beta-D-lactopyranoside
CAS :Chromogenic substrate for cellobiohydrolase and endoglucanase.Formule :C18H25NO13Degré de pureté :Min. 99.0 Area-%Masse moléculaire :463.40 g/molHippurate hydrolase from Campylobacter jejuni, aqueous solution with glycerol
Hippurate hydrolase is an enzyme that catalyzes the hydrolysis of hippurate to glycine and taurine. It is a fluorogenic substrate with high purity, ligand, and high quality. Hippurate hydrolase is used as a diagnostic enzyme for detecting the presence of hippuric acid in urine. This enzyme can be conjugated to a variety of molecules, including fluorophores, biotin or haptens, for use in immunoassay or other applications.Formule :C1944H3000N516O573S18Degré de pureté :Min. 95%Masse moléculaire :24,542 g/molAquaSpark® 510 Calibration Probe, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191
CAS :AquaSpark® Calibration Probe is a water-soluble dioxetane-based chemiluminescent probe. Deprotonation of the free phenol group of the calibration probe under mild basic conditions results in an emissive decomposition process. No addition of enhancer or sensitizing agent is required to produce the bright green light emission in aqueous solutions.
AquaSpark® Calibration Probe is used for:
Demonstration experiment susing only the probe and an aqueous solution or water;
Evaluation of the AquaSpark® maximum light emission;
Functional tests of luminometers, plate readers and cameras;
Comparison with other probes bearing an enzyme labile group in order to build concentration/response curves;
Validation and comparison between different AquaSpark® probes.Formule :C21H23ClO6Degré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :406.12 g/mol5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside
5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-arabinofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is used in bacterial arabinan degradation assays (e.g. Bacillus spp.).Formule :C13H13BrClNO5Degré de pureté :Min. 98.0 Area-%Couleur et forme :PowderMasse moléculaire :378.6 g/molAldol® 455 alpha-D-glucopyranoside - Biosynth Patent: EP 2427431 and US 8940909
Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.
Formule :C27H24ClNO7Degré de pureté :Min. 95 Area-%Couleur et forme :PowderMasse moléculaire :509.93 g/mol4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate
CAS :4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.Formule :C11H21N2O9PDegré de pureté :Min. 95 Area-%Masse moléculaire :356.26 g/molIsopropyl-β-D-thiogalactopyranoside - non-animal origin
CAS :A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.Formule :C9H18O5SDegré de pureté :Min. 99.0 Area-%Masse moléculaire :238.3 g/mol4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-β-D-glucopyranoside
4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.Formule :C24H26O11SDegré de pureté :Min. 95%Couleur et forme :White to off-white solid.Masse moléculaire :522.52 g/mol4-Methylumbelliferyl caprylate
CAS :Fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Used for the identification of Salmonella spp. based on the detection of caprylate esterase.Formule :C18H22O4Degré de pureté :Min. 98 Area-%Masse moléculaire :302.37 g/mol
