Substrats d'enzymes

5-Bromo-4-chloro-3-indoxyl oleate is a fluorescent substrate that is used for staining and enzyme substrate. It is a conjugate that is used in environmental testing, food testing, and diagnostics. 5-Bromo-4-chloro-3-indoxyl oleate has a CAS number of 341972-97-2 and its molecular weight is 229.5 g/mol. It's purity is > 98%, with a specific optical rotation of +147° (c=1, CHCl3). This compound can be used as a chromogenic or fluorogenic substrate in bioluminescence assays. 5BRIO3 has been shown to be an excellent ligand for the detection of DNA polymerase I activity.

Chromogenic substrate for alkaline and acid phosphatase

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5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-rhamnosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is used in bacterial rhamnose metabolism studies (e.g. E. coli).

Chemiluminescent phosphatase substrate in a wide pH range (pH 6 - 10)

Used for asymmetric ketone reduction into chiral alcohols

Methyl 4-methylumbelliferyl hexyl phosphonate is a lipase suicide inhibitor that is a fluorogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases and the fluorescent signal can be detected at Ex 363 nm and Em 465 nm.

3,3'-Diaminobenzidine tetrahydrochloride or DAB hydrochloride is the hydrochloride salt of biphenyl tetra-aniline. DAB hydrochloride is a water-soluble formulation of DAB and is used in immunohistochemical staining of nucleic acids and proteins. DAB hydrochloride is used in Alzheimer's disease research, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize causing a colour change. 3,3’-Diaminobenzidine tetrahydrochloride is also available as the tetrahydrochloride hydrate and as the free base.

The AquaSpark® 510 Nitroreductase/NADH Probe (CL-NTR) is a water-soluble probe for the selective chemiluminescent detection of nitroreductase (NTR) activity. It is a versatile tool for monitoring a number of biological processes.

4-Nitrophenyl 6-O-benzoyl-3,4-O-isopropylidene-a-D-galactopyranoside (4NBP) is a fluorogenic substrate for β-galactosidase. It has been used as an enzyme substrate in the detection of β-galactosidase activity. 4NBP is also used to detect the presence of galactose in culture media and as a chromogenic substrate in diagnostics.

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Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.