CAS 23491-45-4

Hoechst 33258

Descrizione:

Hoechst 33258 è un colorante fluorescente comunemente usato in biologia molecolare e biochimica per colorare il DNA. È caratterizzato dalla sua capacità di legarsi specificamente al solco minore del DNA a doppia elica, il che consente la visualizzazione degli acidi nucleici sotto luce ultravioletta. Il composto mostra una forte fluorescenza al legarsi, rendendolo uno strumento prezioso per applicazioni come la citometria a flusso, la microscopia a fluorescenza e l'analisi del ciclo cellulare. Hoechst 33258 è tipicamente utilizzato in saggi su cellule vive e fisse grazie alla sua natura permeabile alle cellule. La sua struttura chimica include un gruppo di bisbenzimidazolo, che contribuisce alle sue proprietà di legame al DNA. Il colorante è generalmente considerato a bassa tossicità, sebbene debbano essere adottate le appropriate precauzioni di sicurezza durante la manipolazione. Inoltre, Hoechst 33258 può essere utilizzato in combinazione con altri marcatori fluorescenti per facilitare l'analisi multiparametrica in vari allestimenti sperimentali. In generale, la sua specificità per il DNA e le proprietà fluorescenti lo rendono un reagente essenziale nella ricerca genetica e cellulare.

Formula: C₂₅H₂₄N₆O·3ClH

InChI: InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key: InChIKey=SMNPLAKEGAEPJD-UHFFFAOYSA-N

SMILES: CN1CCN(C=2C=C3NC(=NC3=CC2)C=4C=C5C(=CC4)N=C(N5)C6=CC=C(O)C=C6)CC1.Cl

Sinonimi:

• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole trihydrochloride
• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)-benzimidazole trihydrochloride
• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)benzimidazole trichloride
• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)benzimidazole-3HCl
• 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole
• 4-[5-[5-(4-Methyl-1-piperazinyl)-2-benzimidazolyl]-2-benzimidazolyl]phenol trihydrochloride
• 4-[6-(4-methylpiperazin-1-yl)-1',3'-dihydro-1H,2'H-2,5'-bibenzimidazol-2'-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride
• Bisbenzimide
• Bisbenzimide (quenchant)
• Bisbenzimide H 33258
• Bisbenzimide trihydrochloride
• H 33258
• Ho 33258
• Hoe 33258
• Hoechst 33258
• NSC 322921
• Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]-, trihydrochloride
• Phenol, 4-[6-(4-methyl-1-piperazinyl)[2,6′-bi-1H-benzimidazol]-2′-yl]-, hydrochloride (1:3)

Bisbenzimide H 33258 (0.2mL*5) (1mg/mL in Water) [for Cell Staining]

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3HCl

Colore e forma: Light yellow to Yellow clear liquid

Peso molecolare: 533.88

Ref: 3B-B6236

Bisbenzimide H 33258 [for Biochemical Research]

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3HCl

Purezza: >98.0%(HPLC)

Colore e forma: Light yellow to Brown to Dark green powder to crystal

Peso molecolare: 533.88

Ref: 3B-H1343

Bis-Benzimide H 33258

CAS: 23491-45-4

Bis-Benzimide H 33258

Formula: C₂₅H₂₄N₆O·3ClH

Purezza: By hplc: 99.37% (Typical Value in Batch COA)

Colore e forma: Solid-Powder

Peso molecolare: 533.88047

Ref: 54-BIH1003

Hoechst 33258 trihydrochloride

CAS: 23491-45-4

Hoechst 33258 trihydrochloride

Formula: C₂₅H₂₄N₆O·3HCl

Purezza: 99.5%

Peso molecolare: 533.9

Ref: 54-BUP16267

Hoechst 33258 trihydrochloride

CAS: 23491-45-4

Formula: C₂₅H₂₇Cl₃N₆O

Purezza: 98%

Colore e forma: Solid

Peso molecolare: 533.8805

Ref: IN-DA00I4K8

Hoechst 33258 trihydrochloride

CAS: 23491-45-4

4-(5-(4-Methylpiperazin-1-yl)-1H,1'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenol trihydrochloride

Formula: C₂₅H₂₇Cl₃N₆O

Purezza: 98%

Peso molecolare: 533.88

Ref: IN-DG133716

Bisbenzimide H 33258 Fluorochrome trihydrochloride

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3HCl

Purezza: ≥ 98.0%

Colore e forma: Yellow to dark yellow powder

Peso molecolare: 533.9

Ref: 7W-GT5082

Hoechst 33258 trihydrochloride

CAS: 23491-45-4

Hoechst 33258 trihydrochloride (Bisbenzimide) is a benzimidazole anti-filarial agent. Hoechst 33258 is fluorescent when it binds to certain nucleotides in DNA.

Formula: C₂₅H₂₇Cl₃N₆O

Purezza: 96.31% - 99.53%

Colore e forma: Yellow Solid

Peso molecolare: 533.88

Ref: TM-T4057

4-{6-[6-(4-methylpiperazin-1-yl)-1H-1,3-benzodiazol-2-yl]-1H-1,3-benzodiazol-2-yl}phenol trihydrochloride

CAS: 23491-45-4

Purezza: 98%

Colore e forma: Solid

Peso molecolare: 533.8800049

Ref: 10-F868889

Hoechst 33258

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3ClH

Colore e forma: Neat

Peso molecolare: 533.88

Ref: TR-H456600

Hoechst 33258

CAS: 23491-45-4

Hoechst 33258 is a fluorescent dye that binds to the DNA of cells and can be used as a model system in cell biology. It is also used to detect radiation exposure and has been shown to inhibit the growth of certain bacteria. Hoechst 33258 binds with high affinity to basic proteins, such as histones, which are rich in arginine. This interaction inhibits the ability of DNA polymerase to synthesize DNA and prevents cellular transformation. The drug also inhibits cellular physiology by inhibiting protein synthesis, which may lead to cell death. Hoechst 33258 is not active against most bacteria because it has low affinity for phosphate groups on the bacterial cell wall.

Formula: C₂₅H₂₄N₆O•(HCl)₃

Purezza: Min. 95%

Colore e forma: Yellow Powder

Peso molecolare: 533.88 g/mol

Ref: 3D-FH36311