soluzioni tampone di pH

<p>Applications An amino acid found in the urine of species of the Felidae family, is believed to either possess pheromone activity or give rise to compounds with such activity.<br>References Daniels, D.L. and Richert, C.: Tetrahedron Letters, 40, 4463 (1999)<br></p>

<p>Tris-HCl is the same as Tris base with the addition of HCl. It is used as a buffering agent to prepare buffer solutions.</p>

<p>Bis-Tris is a Bis(2-hydroxyethyl) amine buffer that forms metal chelates and can be used with proteins and nucleic acids. This buffering agent has an optimal pH range of 5.8-7.2 and a pKa of 6.46.</p>

<p>2-(N-Cyclohexylamino)ethanesulfonic acid, also known as CHES, is a biological cyclohexylamino buffer with an optimal pH range of 8.6-10.0 and a pKa of 9.5. It has poor metal ion coordination and is suitable for applications above physiological pH.</p>

<p>HEPPS, also known as EPPS, is a piperazinic buffer with an optimal pH range of 7.3-8.7 and a pKa of 8. This pH range is suitable for phosphorylation reaction studies.</p>

<p>HEPES, also known as 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a piperazinic buffer with an optimal pH range of 6.8-8.2 and a pKa of 7.48. This zwitterionic buffering agent is widely used in cell culture however when exposed to light HEPES can produce hydrogen peroxide.</p>

<p>Tris is a buffering agent with an optimal pH range of 7.1-9.1 and a pKa of 8.07. It is used to prepare buffer solutions within physiological pH range and is suitable for western blot and nucleic acid electrophoresis. This buffer also forms metal chelates.</p>

<p>3-Cyclohexylamino-1-propanesulfonic acid sodium, also known as CAPS sodium salt, is a zwitterionic biological buffering agent with an optimal pH range of 9.7 to 11.1 and a pKa of 10.4. It is useful for the extraction of proteins from biological samples and for studying enzyme kinetics at high pH.</p>

<p>MOPS is a morpholinic buffer with an optimal pH range of 6.5-7.9 and a pKa of 7.14. It does not coordinate metal ions and can be used in cell culture and electrophoresis.</p>

<p>Tricine is a buffering agent that is widely used in electrophoresis and ATP assays. It has an optimal pH range of 7.4-8.8 and a pKa of 8.15.</p>

<p>3-(Cyclohexylamino)-1-propanesulfonic acid, also known as CAPS is a zwitterionic biological buffering agent with an optimal pH range of 9.7 to 11.1 and a pKa of 10.4. It is useful for the extraction of proteins from biological samples and for studying enzyme kinetics at high pH.</p>

<p>HEPES sodium salt is a piperazinic buffer with an optimal pH range of 6.8-8.2 and a pKa of 7.48. This buffering agent is widely used in cell culture however when exposed to light HEPES can produce hydrogen peroxide.</p>

<p>TAPS, also known as 3-[(Tris(hydroxymethyl)methyl)amino]-1-propanesulfonic acid, is a buffering agent that can be used in capillary electrophoresis with DNA and can form metal complexes. It has an optimal pH range of 7.7-9.1 and a pKa of 8.4.</p>

<p>BES, also known as 2-[N,N-Bis-(2-hydroxyethyl)amino]ethanesulfonic acid, is a buffering agent that forms complexes with DNA and certain metal ions, It has an optimal pH range of 6.4-7.8 and a pKa of 7.09.</p>

<p>RIPA (Radio-Immuno Precipitation Assay) Buffer is a ready- to-use versatile solution and widely used lysis buffer for preparing cell lysates for a variety of protein-related applications. Radio Immuno Precipitation Assay buffer (RIPA buffer) is used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structuresProtein lysis can be completed within 45 to 60 minutes. </p>

<p>DNA Methylation reagent plays a key role in DNA methylation analysis. It works by selectively converting unmethylated cytosines to uracil, while leaving methylated cytosines unchanged. This difference in conversion allows researchers to identify and analyze DNA methylation patterns using subsequent PCR amplification and sequencing.</p>

<p>DNA Quick Extraction Reagent is used to isolate genomic DNA from various samples. The reagent efficiently extracts DNA from sources like animal &amp; plant tissues, yeast, and bacteria. The process typically takes 10-30 minutes, with DNA recoveries ranging from 70-100%.</p>

<p>A ready-to-use 2X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. <br> Role of 2X SDS PAGE Sample buffer :<br> 1 .Tris-HCl is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.<br> 2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.<br> 3. The high density of glycerol ensures the sample moves down into the well.<br> 4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.<br> 5. The dye visually indicates the location (tracking) of the sample in the gel. </p>

<p>It is the most commonly used sample buffer for SDS-PAGE of denatured proteins. It is a ready-to-use 5X solution with dithiothreitol (DTT) as a reducing agent. It can be used for SDS-PAGE protein loading of conventional proteins. The use of sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. :It is the most commonly used sample buffer for SDS-PAGE of denatured proteins. It is a ready-to-use 5X solution with dithiothreitol (DTT) as a reducing agent. It can be used for SDS-PAGE protein loading of conventional proteins. The use of sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. :It is the most commonly used sample buffer for SDS-PAGE of denatured proteins. It is a ready-to-use 5X solution with dithiothreitol (DTT) as a reducing agent. It can be used for SDS-PAGE protein loading of conventional proteins. The use of sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer.Role of reagents: <br> Tris-HCl: as a buffering substance. The pH play an essential role in preserving peptide bonds from breaking apart. <br> SDS: Proteins comes in different sizes and charges. SDS is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge. <br> Glycerol: The high density (thickening of the solution) of glycerol ensures the sample moves down into the well. <br> DL-Dithiothreitol (DTT): is a strong reducing agent. Its specific role in sample denaturation is to remove the last bit of tertiary and quaternary structure by reducing disulfide bonds.<br> Bromophenol Blue: visually indicates the location (tracking dye) of the sample in the gel. </p>

<p>It is the most commonly used sample buffer for SDS-PAGE of non-denatured proteins It is a ready-to-use 5X solution without dithiothreitol (DTT) and Beta-mercaptoethanol (BME) as a reducing agent. This buffer can be used as a loading buffer in all kinds of denaturing gels and are compatible with Coomassie, silver staining and Western blotting. The 5X concentration of this buffer facilitates the loading of larger sample volume per well compared to the traditional 2X loading buffer.Role of reagents: <br> Tris-HCl: as a buffering substance. The pH play an essential role in preserving peptide bonds from breaking apart. <br> SDS: Proteins comes in different sizes and charges. SDS is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge. <br> Glycerol: The high density (thickening of the solution) of glycerol ensures the sample moves down into the well. <br> Bromophenol Blue: visually indicates the location (tracking dye) of the sample in the gel. </p>

<p>A ready-to-use 5X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. Role of 5X SDS PAGE Sample buffer :1. Tris is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.<br> 2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.<br> 3. The high density of glycerol ensures the sample moves down into the well.<br> 4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.<br> 5. The dye visually indicates the location (tracking) of the sample in the gel. </p>

<p>20X Borate Buffer is a stock solution that is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at alkaline pH. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Borate Buffer pH remains 8.5, when diluted to 1X with water.</p>