Substrati enzimatici

AquaSpark® Alkaline Phosphatase Substrate is a stand-alone reagent - no additives such as enhancers or detergents are required to start a strong light reaction. AquaSpark® Alkaline Phosphatase Substrate is superior to other luminogenic alkaline phosphatase substrates as it shows significantly higher signal intensities and very low background.Application Examples:

6-Bromo-2-naphthyl-alpha-D-mannopyranoside is a strong inhibitor of alpha-mannosidase. It has been shown to inhibit the growth of Mycobacterium tuberculosis and Streptococcus pneumoniae, which are both known to cause bacterial infections in humans. 6BNA inhibits the enzyme alpha-mannosidase, which hydrolyzes terminal α-(1,4)-linked mannose residues from oligosaccharides and glycoproteins. This drug has a high affinity for the active site of alpha-mannosidase and an IC50 value of 0.2 mM, making it an effective inhibitor.

6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin is a fungicide that inhibits the growth of fungi by interfering with their DNA synthesis. It does this by inhibiting the activity of two enzymes: the fungal enzyme oxime and the enzyme cinerea, which is found in many fungi. 6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin has been shown to be effective against Alternaria and Botrytis cinerea. The compound also has an antifungal effect against other types of fungi, such as Penicillium roqueforti, when used in combination with a solvent such as ether.

3-Carboxyumbelliferyl b-D-galactopyranoside is a fluorescent probe that can be used to study protein synthesis in live cells. It binds to the active site of the enzyme peptidyl transferase, which catalyzes the covalent linkage of amino acids to form a polypeptide chain. 3CMBG is specific for the amino acid proline and is a substrate for peptidyl transferase. The fluorescence of 3CMBG increases when it is bound to peptidyl transferase, making it useful as a marker for protein synthesis.

4-Trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid is a fluorescent chemiluminescent substrate that is used in the detection of beta-galactosidase activity. Beta-galactosidase catalyzes the hydrolysis of 4-trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid to form 4-trifluoromethylumbelliferone and D-galactose, which emits light at approximately 480 nm. This reaction can be detected by fluorescence spectrophotometry using a fluorometer or photometer. 4TFUNA is not toxic, does not inhibit enzymes, and has been tested for toxicity and mutagenicity using bacteria and mammalian cells.

4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-b-D-glucopyranoside is a fluorogenic substrate specifically designed for the detection and quantification of hexosaminidase enzymes. Upon cleavage by the target enzyme, it generates a bright fluorescent signal facilitating the investigation of enzyme activity, screening of inhibitors, and diagnostics.

2-Nitrophenyl 2,3-di-O-acetyl-b-D-xylopyranoside is a chromogenic substrate used to assay the activity of xylosidase enzymes. It is hydrolyzed by xylosidase to yield a yellow-colored product, 2-nitrophenol, which absorbs light at 405 and 410 nm. This substrate is used in research to study the function of xylosidase enzymes and their role in various biological processes. It is also used in diagnostic tests for the detection of xylosidase activity in different microorganisms, including bacteria and fungi.

5-Bromo-4-chloro-3-indoxyl sulfate p-toluidine salt is a staining agent used for the detection of glucose in urine and tissues. It is also an enzyme substrate that can be used to detect the presence of lipases, amylases, esterases, phosphatases, and proteases. This product has a CAS number of 6581-23-3 and comes in high quality.

4-Bromo-2-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that reacts with an excess of luciferase to produce light. It is used as a reagent in the detection of ATP, NADH, and other compounds in biochemistry and biology. 4-Bromo-2-nitrophenyl b-D-galactopyranoside is also used in diagnostic kits for detecting bacterial or fungal contamination in food.

This product is a fluorogenic substrate that reacts with the enzyme beta-galactosidase to produce a fluorescent product. It can be used in food testing, environmental testing, and diagnostics. The CAS number for this product is 609-33-2. This product is available in high purity and high quality.

4-Methylumbelliferyl 4-deoxy-b-D-chitobiose peracetate is a chromogenic substrate used for the detection of β-galactosidase activity. It is an analog of 4-methylumbelliferone that can be hydrolyzed by β-galactosidase to produce fluorescence. This product is supplied as a white crystalline solid and can be stored at room temperature. This substrate has been shown to work well in culture media, food testing, and environmental testing.

Aldol® 515 palmitate is a high quality, non-toxic and water soluble chromogenic substrate for enzymes. It is used to detect the presence of lipase activity due to yielding a red color.

2-Chloro-4-nitrophenyl b-D-xylobioside is a chemiluminescent substrate that is used as a diagnostic agent. It has been used in the identification of gram positive and gram negative bacteria, which are differentiated by the color of the reaction. This substrate has also been used to detect the presence of certain enzymes produced by bacteria. 2-Chloro-4-nitrophenyl b-D-xylobioside has shown to be an effective enzyme substrate for detecting bacterial growth in culture media and is often used as a fluorogenic substrate in food testing.

2-Chloro-4-nitrophenyl a-D-fucopyranoside (CNPF) is a chromogenic enzyme substrate used primarily to detect fucosidase activity. CNPF is cleaved by the enzyme, which results in the release of a colored product that can be measured spectrophotometrically. Fucosidases are important enzymes that catalyze the hydrolysis of fucose-containing oligosaccharides, and CNPF is a commonly used substrate for the detection and quantification of these enzymes in various research studies.

2-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)]--2-deoxy--bDglucopyranoside is an acylated glucopyranoside that is used as a substrate for the enzyme β -galactosidase. When hydrolyzed by β -galactosidase, it yields a blue product that has been widely used in chemiluminescence and bioluminescence assays. It also can be used for staining and ligand binding studies.

5-Bromo-6-chloro-3-indolyl palmitate is a magenta enzyme substrate that is widely used in the study of lipase and esterase enzymes. This compound consists of an indole ring with bromine and chlorine atoms at positions 5 and 6, respectively, and a palmitate moiety esterified to the indolyl ring. The palmitate group is a long-chain fatty acid, making this substrate particularly useful for investigating enzymes that hydrolyze ester bonds in lipids. Upon cleavage by lipases or esterases, 5-Bromo-6-chloro-3-indolyl palmitate releases a magenta-colored product, which can be easily detected and quantified to measure enzyme activity.

Aldol® 515 beta-D-xylopyranoside is a chromogenic substrate that is used in food testing. It is an alternative to the more common glucose and sucrose, which are not suitable for some types of assays. Aldol® 515 beta-D-xylopyranoside is also used in diagnostics as a ligand for fluorescent antibody staining and chemiluminescence assays. It has been shown to increase the sensitivity of these tests by up to 10 times. The substrate has been tested extensively and is safe for use with a wide range of bacteria including E. coli, S. typhimurium, M. tuberculosis, and C. albicans at concentrations up to 100 mM without any inhibitory effects on growth or viability.
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Aldol® 515 L-proline amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-proline aminopeptidase activity. The colorless enzyme substrate is cleaved by L-proline aminopeptidase produced by microorganisms grown in liquid or solid media, yielding red-orange color. In addition, red fluorescence signals are generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-proline amide is suitable for use under aerobic and anaerobic conditions.

2-Bromo-4-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that is used for the detection of β-galactosidase activity in the biochemical reaction. It has been shown to be an excellent ligand for metal ions and can be used as a reagent in the determination of metal ions in biological systems. This product is manufactured with high purity and quality, which ensures its suitability for use in culture media, environmental testing, and diagnostics. 2-Bromo-4-nitrophenyl b-D-galactopyranoside has been tested as a fluorogenic substrate for β-galactosidase. It has also been used as a chromogenic substrate for β-galactosidase and an enzyme substrate for β-galactosidase. This product can be conjugated with other molecules such as biotin to enable rapid analysis of proteins or

1-Naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate commonly used to assay the activity of enzymes that cleave N-acetyl-beta-D-glucosaminide, such as beta-N-acetylglucosaminidase. Upon cleavage by the enzyme of interest, the substrate releases a yellow-colored 1-naphthylamine, which can be measured spectrophotometrically. This substrate is widely used in biomedical research and drug discovery for studying lysosomal storage disorders, bacterial infections, and neurological diseases.

L-Citrulline-p-nitroanilide hydrobromide is a substrate for enzymes such as beta-galactosidase, beta-glucuronidase, and alkaline phosphatase. It is used in biochemical assays for the detection of microorganisms and in medical diagnostics. This compound has been shown to be useful in fluorescence microscopy and bioluminescence. It also has applications in food testing, environmental testing, and high purity research.

5-Bromo-6-chloro-1H-indol-3-yl-a-D-mannopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lysosomal storage disorder screening.

4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2,6,8,10,12-pentaoxoheptaacetic acid is a chemiluminescent substrate that is used for the detection of peroxidase and other enzymes. The reaction product is a yellow fluorescent product which can be measured using a fluorometer. It is sold as an aqueous solution at 1mg/mL in phosphate buffer at pH 7.4.

4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a useful substrate for enzymatic assays. This high-sensitivity chromogenic pNP substrate offers enhanced selectivity and increased detection ability, allowing for a broad range of applications in the field of glycosidase research. Featuring rapid colorimetric readouts with easily detectable endpoint determination, this substrate delivers clear and accurate results every time.

N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.