Substrati enzimatici

Chlorophenol red-b-D-galactopyranoside sodium salt is the salt from of CPRG (chlorophenol red-b-D-galactopyranoside), that has a higher solubility. It is a chromogenic substrate for beta-galactosidases that, after enzymatic cleavage, releases chlorophenol red, a dark red compound quantified by absorbance at 570 nm.

Chromogenic β-glucosaminidase substrate yielding a yellow solution upon cleavage, used particularly in yeasts and molds.

DMNPE-caged Luciferin is a photoactivatable probe that can easily penetrate the cell membrane, and by exposure to UV light it undergoes a photochemical decomposition liberating a Luciferin molecule. Once exposed to Luciferase enzyme expressed by the cells, Luciferin emits visible (and measurable) light. Using_x000D_this technique, imaging of luciferase activity was performed both in living cells and in mouse tumor xenograft, by intraperitoneal injection of the caged DMNPE luciferin (Chem. Commun., 2009, 0, 4028-4030).

4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is a synthetic substrate that is used in enzyme kinetics. It is a beta-galactosidase substrate that reacts with the enzyme to form a product. The rate of reaction can be measured by following the change in absorbance over time. This product will react with an acceptor, such as chondroitin sulfate, to produce an observable reaction product. 4NPBGA has been shown to be an effective carbon source for plant physiology studies and has been shown to have optimal activity at pH 6.5.

<p>Please enquire for more information about 5-Nitro-3-indoxyl-β-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>A chromogenic and fluorogenic substrate, used for detection of esterase activity.</p>

<p>Chemiluminescence beta-galactosidase substrate</p>

<p>Chromogenic substrate for α-D-Fucosidase yielding a blue precipitate.</p>

Fluorogenic substrate for α-L-arabinosidase yielding a blue fluorescent solution upon cleavage.

Chromogenic α-mannosidase substrate yielding a absorbent solution upon cleavage. Also used for studies of the crystal structure of the complexes of concanavalin A and binding studies with concanavalin A.

6-Fluoro-3-indoxyl-beta-D-galactopyranoside is a fluorescent chromogenic substrate that is commonly used for the detection and quantification of beta-galactosidase activity. When this substrate is hydrolyzed by beta-galactosidase, it produces a fluorescent blue product that can be easily detected and measured using a fluorometer or spectrophotometer. This substrate is often used in microbiology and molecular biology research to study gene expression and enzyme kinetics, and is also used in medical diagnostics as a marker for specific diseases and conditions.

<p>3-Indoxyl phosphate, p-toluidine salt is a fluorogenic substrate that can be used for the detection of β-galactosidase. 3-Indoxyl phosphate, p-toluidine salt is a highly active chromogenic substrate that can be used as a diagnostic tool in the food industry to determine whether or not milk has been adulterated with animal milk. This product is also used in environmental testing to detect bacteria and fungi. 3-Indoxyl phosphate, p-toluidine salt is a high purity, high quality conjugate that can be used for the detection of β-galactosidase.</p>

Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.

Chromogenic substrate for α-L-arabinofuranosidase yielding a yellow solution upon cleavage.

4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).

Please enquire for more information about Resorufin-alpha-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

<p>Please enquire for more information about Trimethyl phosphonoacetate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

L-Leucine-PAB-resorufin is a substrate for enzymes such as β-glucuronidase, α-glucosidase, and β-galactosidase. It is used in the diagnosis of bacterial infections and the detection of certain bacteria in urine and stool samples. L-Leucine-PAB-resorufin can be used to detect the presence of bacteria that produce β-galactosidase and/or β-glucuronidase by releasing resorufin from the substrate. This reaction produces a blue color in the presence of a fluorogenic substrate like fluorescein diacetate or fluorescein isothiocyanate. L-Leucine-PAB-resorufin is also used to detect bacteria that produce α-glucosidase by releasing fluorescamine from the substrate. This reaction produces a green color in the presence of a chrom

Use as a positive control when detecting nitroreductase activity

A substrate for beta-glucuronidase.