Substrati enzimatici

<p>Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases.</p>

<p>Please enquire for more information about 2,5-dioxabicyclo[2.2.1]heptan-7-one including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Please enquire for more information about 3-Indoxyl myo-inositol-1-phosphate, ammonium salt including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Methylumbelliferyl 4-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 4-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis and in screening studies of glycoprotein metabolism disorders.</p>

<p>Luc-phosphate Na enhanced is a bioluminescent substrate used to detect phosphatase activity in bacterial assays.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.</p>

<p>4-Trifluoromethylumbelliferyl-beta-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. It is used in the detection of beta-galactosidase activity in cell lysates and culture media, as well as in food testing. This compound has been shown to be highly pure, with a purity greater than 98%. 4-Trifluoromethylumbelliferyl-beta-D-galactopyranoside has also been shown to have high chemiluminescence, making it an ideal candidate for use in diagnostics.</p>

<p>Bioluminescent substrate for luciferase</p>

<p>Histochemical substrate for esterase</p>

<p>Selenium analogue of firefly luciferin with red-shifted bioluminescence</p>

<p>For detection of beta-galactosidase activity. <br>The colors of the chromogen can be adjusted by incorporating different functional groups, e.g. cyano or nitro group, which yields purple or black green colonies with beta-galactosidase positive strain.</p>

<p>Chromogenic substrate targeting alpha-L-arabinofuranosidase</p>

<p>Esculetin-7-beta-D-galactopyranoside, also known as 6,7-Dihydroxycoumaryl-7-beta-D-galactopyranoside, is a fluorogenic substrate used in the detection of beta-galactosidase activity. This coumarin derivative, when hydrolyzed, yields esculetin (6,7-dihydroxycoumarin) which, in the presence of iron salts, forms a brown-black complex.</p>

<p>Chromogenic substrate to visualize the activity of alpha-D-mannopyranoside; red color produced</p>

<p>Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranosideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B screening and mucopolysaccharidosis diagnosis.</p>

<p>Chromogenic sulfatase substrate</p>

<p>3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that is used for the detection of beta-galactosidase. It has been shown to be an efficient substrate for the enzyme beta galactosidase and can be used to detect this enzyme in cell culture media and other diagnostic applications. 3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside can also be used as a chromogenic substrate, which is added to a reaction mixture containing beta galactosidase, where it is hydrolyzed by the enzyme to produce a blue color. This product also has food testing and environmental testing applications.</p>

<p>4-Nitrophenyl beta-L-arabinopyranoside is a chromogenic substrate for beta-L-arabinosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-L-arabinopyranoside is used in different applications such as the Bacterial arabinan degradation assays, gut microbiome analysis</p>

<p>4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside is an enzyme substrate that is used in the detection of phenylalanine, tyrosine, and tryptophan. It is also used for environmental testing, bioluminescence, culture media, chemiluminescence, fluorogenic substrate, food testing, diagnostics and other applications.</p>

<p>1-Naphthyl b-D-galactopyranoside is a chromogenic substrate used to detect the presence of beta-galactosidase enzymes. The enzyme cleaves the substrate to produce a colored product that can be quantified and visualized. This substrate is commonly used in applications such as the detection of lacZ gene expression in bacterial colonies or in histochemical staining of tissues to identify beta-galactosidase activity.</p>

<p>6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.</p>

<p>Dehydroluciferin is a bioluminescence probe that is a potent inhibitor of the firefly luciferase reaction. It is a byproduct of the oxidation of luciferin, the substrate for firefly luciferase, which catalyzes the bioluminescence reaction. It can also form during the synthesis or storage of luciferin. Its presence can potentially lead to issues in bioluminescent assays.</p>

<p>Substrate for a-L-arabinopyranosidase</p>

<p>4-Nitrophenyl a-D-mannopyranoside is a chromogenic pNP enzyme substrate that enables the detection and quantification of a-D-mannosidase activity in various biological samples through a colorimetric assay. The hydrolysis of the substrate by the enzyme releases 4-nitrophenol, a yellow compound whose absorption can be measured spectrophotometrically. Applications for this substrate span biomedical research, disease diagnostics, and industrial enzyme production.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Chromogenic substrate for beta-glucuronidase</p>

<p>5-Nitro-3-indolyl-b-D-galactopyranoside is a substrate for a number of enzymes, including beta-galactosidase, beta-glucuronidase, and beta-mannosidase. It is used as an enzyme substrate in diagnostics to measure the activity of these enzymes. 5N3IG was also used to study the effects of conjugation on enzyme activity. 5N3IG is available in high purity and high quality at CAS No.</p>

<p>Beta-naphthyl stearate is a chromogenic substrate for measuring enzymatic activity.</p>

<p>Aldol® 518 alpha-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of alpha-galactosidase activity. The enzyme substrate is cleaved by alpha-galactosidase positive bacteria and yeasts in liquid or solid media, yielding red color and red fluorescence signal. Aldol® 518 alpha-D-galactopyranoside can also be used to detect alpha-galactosidase (encoded by the Mel1 gene) in yeast gene reporter assays or for identification of alpha-gal positive transformants on agar plates. Aldol® 518 alpha-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>2-Chloro-4-nitrophenyl a-D-maltotrioside (2CNP) is a potent hypoglycemic agent that has been shown to decrease postprandial blood glucose levels in humans. 2CNP is a white crystalline solid that is soluble in water and ethanol. The transfer reactions of 2CNP are enhanced by benzalkonium chloride, which forms an organic complex with the drug. The optimum concentration for the hypoglycemic effect of 2CNP is determined to be 10 μM, which can be detected using an optical sensor. This compound also inhibits α-amylase and other enzymes involved in carbohydrate metabolism, leading to the accumulation of glycogen and lowering the blood glucose level.</p>

<p>Chromogenic substrate for beta-galactosidase</p>

<p>Fluorogenic substrate for α-D-glucosidase. It has been used in studies of Pompe's disease (Glycogen Storage Disease). Used for differentiation and enumeration of Enterobactersakazakii based on α-glucosidase activity.</p>

<p>Luc-Salmonella is a conjugate of the enzyme luciferase and the substrate 5-bromo-4-chloro-3-indolyl phosphate. Luc-Salmonella has been used for diagnostic purposes in the detection of bacteria in culture media, as well as in environmental and food testing. Luc-Salmonella can be used to detect Salmonella, which is a bacterium that can cause gastrointestinal disease. Luminescence is measured using a luminometer or fluorimeter with an excitation wavelength of 485 nm and emission wavelengths at 510 nm. Luminescence from Luc-Salmonella is typically measured after 1 hour incubation with bacterial cells.<br>Luc-Salmonella has been shown to have high purity and quality due to its unique composition of enzyme substrate. This product has been tested for contamination by sensitive assays such as chemiluminescence, bioluminescence, or fluorescence. The purity level of Luc-Sal</p>

<p>4-Methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotriosideas a substrate for measuring the chitinase activity is used for fungal infection assays.</p>

<p>Coelenterazine is a bio-luminescent substrate of luciferases such as Renilla luciferase (Rluc) and Gaussia luciferase (Gluc). The main applications of Coelenterazine are monitoring of reporter genes and chemiluminescence-based methods for Calcium detection. The substrate is also used in bioluminescence resonance energy transfer (BRET), ELISA, HTS and chemiluminescent detection of superoxide anion and peroxynitritein cells or tissues. About this grade Coelenterazine, native, dried (C-7002): in order to provide milligram and sub-milligram quantities of native Coelenterazine we pre-dissolve the substance; defined amounts of Coelenterazine are subsequently dried under nitrogen in individual vials. Coelenterazine, native, dried is identical in quality and chemistry to our product Coelenterazine, native which is provided as a powder.<br>Information to the user: since Coelenterazine is sensitive to oxygen, degas your solvents for stock solutions, if possible. To dissolve Coelenterazine, native, dried please proceed as follows. 1) Add the appropriate amount of solvent. If possible through the septum using a needle. Otherwise open the cap with pliers. 2) Vortex the closed vial briefly to completely dissolve the content of the vial. 3) Take out the solution, if possible with a needle through the septum.</p>

<p>This Clips-O substrate 7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin allows the detection of single and dual enzymatic activity (L-alanine aminopetidase and oxidation of 4-amino-3-hydroxybutyloxy spacer either enzymatically or by exposure to sodium periodate) with a single substrate resulting in a fluorescent signal. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.</p>

<p>5-Bromo-4-chloro-3-indolyl palmitate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl palmitate is used in lipase activity assays or cystic fibrosis diagnostics.</p>

<p>Aldol® 518 alpha-D-glucopyranoside is a fluorogenic enzyme substrate used for detection of alpha-D-glucosidase activity. The colorless enzyme substrate is cleaved by alpha-D-glucosidase, generating red fluorescent signal and in some conditions, an orange colour in the visible spectrum can get formed, staining bacterial colonies in faint orange coloration. Aldol® 518 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>DL-threo-2-methylisocitrate sodium salt is an enzyme substrate for isocitrate lyase 1 (ICL1), an enzyme involved in the glyoxylate cycle, a metabolic pathway that allows organisms to convert acetate into glucose. It is also a substrate for bovine heart aconitase and acts as inhibitor of TPN-linked isocitrate dehydrogenase.</p>

<p>L-Serine 7-amido-4-methylcoumarin hydrochloride is a fluorogenic peptide substrate for peptidases. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.</p>

<p>6-(b-D-Galactopyranosyloxy)-9-(4-methoxy-2-methylphenyl)-3H-xanthen-3-one (MGBG) is a hydrophilic activatable fluorescence probe that has been shown to be effective in photodynamic therapy. MGBG is activated by light and has a high affinity for esterases, which are found in many tissues. The fluorescence of the MGBG is quenched by the esterase enzymes, but when it is exposed to light and oxygen, the probe becomes activated and its fluorescence increases. MGBG can be used as a fluorescent probe for intraperitoneal tumor resection or as a fluorescent imaging agent in optical microscopy.</p>

<p>Fluorogenic substrate for phosphatidylinositol-specific phospholipase C. Isolation and identification of Listeria monocytogenes, bacteria of the Bacillus cereus group, yeasts and molds. See also B-7404.</p>

<p>Aldol® 515 acetate is a chromogenic and fluorogenic substrate, used for detection of esterase activity. The colorless product is cleaved by purified esterase, or esterase secreted by microorganisms in liquid or solid media, yielding a red color and red fluorescence signal when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 acetate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of a purified esterase, color and fluorescence develops within minutes to few hours.</p>

<p>4-MU-Gal-6S (M4A) is a fluorogenic substrate of galactose-6-sulfate sulfatase (GALNS), used for the diagnosis of Mucopolysaccharidosis IV type A (MPS IV A). MPS IV, also known as Morquio syndrome, is a rare metabolic disorder in which the human body cannot process glycosaminoglycans (GAGs). MPS IV A is caused by the deficiency of the enzyme N-acetyl-galactosamine-6-sulfatase resulting in accumulation of keratan and chondroitin sulfate in tissues. M4A is used as the GALNS substrate in the anti-lysosomal enzyme antibody assay for the treatments of lysosomal storage disorders (LSDs) with enzyme replacement therapies (ERTs).</p>

<p>2-Naphthyl caprylate is a substrate that is employed in an assays for carboxylesterase and lipase and when hydrolyzed, produces 2-naphthol, a compound that can also be identified via fluorescence analysis. By combining it with a diazonium salt, the formation of the corresponding azo dye occurs. In particular, this substrate is used to detect the extracellular lipase of Pseudomonas fluorescens B52.</p>

<p>Resorufin b-D-glucopyranoside is a stable red fluorogenic galactosidase substrate.</p>

<p>Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>L-Luciferin is an enzyme substrate that is used in the detection of bioluminescence. It is a fluorogenic substrate that can be used to detect the presence of glucose in urine. L-Luciferin is also a chemiluminescent substrate that can be used to detect ATP, which is required for cell metabolism. L-Luciferin has been shown to be stable at pH levels ranging from 2.5 to 9 and can be stored for up to 3 years when stored at 4°C with no light or oxygen exposure. This product has CAS No. 754978-59-1, indicating high purity and quality. L-Luciferin will not stain or react with other compounds, making it highly suitable for use as a conjugate in diagnostic tests or as a ligand in fluorescent staining reactions.</p>

<p>5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).</p>

<p>4-Methylumbelliferyl phosphocholine, commonly known as 4-MUPC, is a synthetic phosphodiester used as an enzyme substrate for phospholipase enzymes. 4-MUPC is used as a fluorogenic substrate to measure the activity of phopholipases, which are important mediators of cell signalling.</p>

<p>2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.</p>

<p>Chromogenic β-Glucosaminidase substrate yielding a yellow absorbent solution upon cleavage.</p>

<p>Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.</p>

<p>3-Nitrophenyl beta-D-glucopyranoside is a chromogenic enzyme substrate commonly used to assay for beta-glucosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be easily detected by spectrophotometry. This substrate is often used in the field of carbohydrate chemistry and biochemistry to study enzyme kinetics and inhibition in both in vitro and in vivo systems.</p>

<p>For use in bacterial detection</p>

<p>Also known as BCIP; can be used for visualization of phosphatase labelled antibodies on Western blots. This method quickly became extremely popular in diagnostics and biology research.</p>

<p>A substrate used for the diagnosis of Sanfilippo disease C (MPS III C).</p>

<p>4-Methylumbelliferyl 2-O-(a-L-fucopyranosyl)-b-D-galactopyranoside is an enzyme substrate used in chromogenic assays of alpha-fucosidases. It is also used for glycoprocessing enzymes in synthesis.</p>

<p>4-Methylumbelliferyl lignocerate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl lignocerateas a substrate for measuring the lipase activity is used for X-linked adrenoleukodystrophy diagnosis and peroxisomal disorder screening.</p>

<p>Substrate for horseradish peroxidase</p>

<p>Water soluble substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry.</p>

<p>4-Methylumbelliferyl beta-D-glucuronide (MUG) is commonly used as a substrate for detecting beta-glucuronidase. MUG is used for identifying E. coli contamination in food, drinking water, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants.</p>

<p>Water soluble substrate for firefly luciferase.</p>

<p>Please enquire for more information about L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Nitrophenyl-beta-L-fucopyranoside is a chromogenic substrate used in the determination of alpha-glucosidase activity. The formation of phenol from 4-nitrophenyl leads to the production of a yellow color that can be quantified by measuring its absorbance at 490 nm. This assay is based on the principle that alpha-glucosidase hydrolyzes sucrose into glucose and fructose, which are then oxidized to form 4-nitrophenol and 4-nitroanomeric acid, respectively. The reaction product is detected by measuring the absorption at 490 nm.</p>

<p>Luc-Enterobacteria Na is a fluorogenic substrate that can be used for the detection of Enterobacteriaceae. This product has been shown to have high purity and a CAS number. Luc-Enterobacteria Na is also conjugated with an enzyme substrate, making it suitable for detecting the presence of bacterial colonies in culture media.</p>

<p>5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.</p>

<p>N-CBZ-L-Lysyl-L-lysyl-L-arginine 7-amido-4-methylcoumarin triacetate is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>Naphthol AS-BI sulphate potassium salt is a modified naphtol AS substrate used for alkaline and acid phosphatase.</p>

<p>3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.</p>

<p>Chromogenic β-glucosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry.</p>

<p>Fluorogenic substrate for aminopeptidase yielding a blue fluorescent solution.</p>

<p>L-Proline 7-amido-4-methylcoumarin hydrobromide is a fluorogenic substrate that is used to measure esterase and peptidase activity in a variety of animal and microbial sources. This compound has been shown to have a lipase activity. L-Proline 7-amido-4-methylcoumarin hydrobromide is also used as an enzyme preparation and as an enzyme source.</p>

<p>Chromogenic substrate for α-galactosidase yielding a blue precipitate. Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifido bacteria species from Lactobacillus species.</p>

<p>4-Methylumbelliferyl 3-deoxy-D-glycero-a-D-galacto-2-nonulosonic acid sodium salt is a fluorogenic substrate that can be used to detect enzyme activity. It reacts with the enzyme β-galactosidase, which hydrolyzes lactose in the presence of oxygen and hydrogen peroxide. The product is 4methylumbelliferone, which emits light in the presence of luciferin and an oxidizing agent. This product is used for diagnostics, food testing, and environmental testing. CAS No. 123269-95-4</p>

<p>6-Chloro-3-indoxyl choline phosphate is a chromogenic substrate for phosphatidylcholine-specific phospholipase C (PC-PLC) for the detection of mibrobial metabolites. The use of this specific substrate permits monitoring the activity of PC-PLC. This an important enzyme for the regulation of steady-state levels of phosphatidylcholine (PC) and diacylglycerol (DAG). Abnormally high levels of PC-PLC activity in various tissues have been linked to many disease progressions.</p>

<p>6-Chloro-1H-indol-3-yl hexadecanoate is a chromogenic substrate for an enzyme that oxidizes it to produce a blue color. It can be used in the diagnosis of bacterial infections, such as tuberculosis and leprosy. 6CHIH is a ligand for various enzymes, including lactate dehydrogenase, which catalyzes the conversion of pyruvate to lactic acid. This compound reacts with oxygen to produce light in the presence of a catalyst. 6CHIH is also used as a fluorescent tag for protein labeling and detection.<br>6CHIH has been shown to be effective in detecting bacteria in food products and water samples using bioluminescence techniques. The high purity and quality of this compound make it suitable for use in diagnostics, culture media, and environmental testing.</p>

<p>6-Bromo-2-naphthyl b-D-glucopyranoside is an enzyme substrate used in chromogenic assays with beta-glucosidases. It has been used in culture media for the identification of Legionella pneumophila.</p>

<p>Fluorogenic substrate for beta-glucosidase yielding a blue fluorescent solution. Used for the detection of beta-glucosidase as an indicator of Enterococci.</p>

<p>4-Methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium salt is a fluorogenic substrate for N-sulfoglucosamine sulfohydrolase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium saltas a substrate for measuring the N-sulfoglucosamine sulfohydrolase activity is used for screening of Mucopolysaccharidosis IIIA (MPS IIIA) and diagnosis of Sanfilippo A syndrome.</p>

<p>1-Naphthyl a-D-galactopyranoside is a chromogenic substrate use to detect alpha-galactosidase activity.</p>

<p>Fluorogenic substrate for α-L-Fucosidase yielding a blue fluorescent solution upon cleavage.</p>