C-Terminal Sortagging-AAA-[Lys(Biotin]

Rif. 3D-CRB1000650

Informazioni sul prodotto

• Nome: C-Terminal Sortagging-AAA-[Lys(Biotin]
• Sinonimi: H-AAA(K/Biot)-NH2
• Marchio: Biosynth
• Descrizione: This peptide is recognised and cleaved by the enzyme Sortase A (SrtA) from-Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA, serves as a nucleophile to cleave the peptide bond between threonine and glycine. Cleavage results in the formation of a thioacyl intermediate between the peptide and SrtA. This intermediate is then resolved by the N-terminus of an (oligo)alanine residues as acyl acceptors, resulting in the creation of a new peptide bond that links the peptide and its biotin tag to the incoming nucleophile.- This method of protein labelling is known as sortagging.This peptide contains an C-terminal biotin tag for detection and purification.

Proprietà chimiche

• Peso molecolare: 584.3 g/mol
• Colore/Forma: Powder