C-terminal Sortagging-[Cys(Sulfocyanine7)]

Rif. 3D-CRB1101496

Informazioni sul prodotto

• Nome: C-terminal Sortagging-[Cys(Sulfocyanine7)]
• Sinonimi: H-GGG(C/Sulfocyanine7)-NH2
• Marchio: Biosynth
• Descrizione: This C-terminal Sortagging peptide acts as a (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the (Sulfocyanine7) fluorescent moiety being attached to the C-terminus of the target protein or peptide.A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA, serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye. This method of protein labelling is known as sortagging.This peptide contains Sulfocyanine7, which is a NIR (near infrared) emitting fluorescent dye.

Proprietà chimiche

• Peso molecolare: 1,121.4 g/mol