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[5-FAM]/[Lys(Dabcyl)]-CoV Main Protease (Mpro) Substrate
Rif. 3D-CRB1101508
| 1mg | 626,00 € | ||
| 500µg | 452,00 € |
Informazioni sul prodotto
- 5fam-AVLQSGFR(K/Dabcyl)K-NH2
FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The substrate sequence is derived from residues P4-P5' of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. SARS-CoV Mpro is a key antiviral target.This peptide contains an N-terminal 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag and the Dabcyl fluorescence quencher. When the peptide is intact, fluorescence is undetectable due to the proximity of the Dabcyl quencher to the 5-FAM fluorophore. However upon cleavage of the peptide by SARS-CoV Mpro, the 5-FAM group and the Dabcyl quencher are separated and fluorescence can be detected. This therefore represents a useful tool for investigating SARS-CoV Mpro activity.
Proprietà chimiche
Richiesta tecnica su: 3D-CRB1101508 [5-FAM]/[Lys(Dabcyl)]-CoV Main Protease (Mpro) Substrate
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