X-Gal

CAS: 7240-90-6

Rif. 3D-EB06680

Informazioni sul prodotto

• Nome: X-Gal
• Sinonimi: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal)X-b-D-GalactosideBCIG, .beta.-D-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl, 5-Bromo-4-chloro-1H-indol-3-yl beta-D-galactopyranoside, 5-Bromo-4-chloro-1H-indol-3-yl β-<span class="text-smallcaps">D</span>-galactopyranoside, 5-Bromo-4-chloro-3-C-indolyl-β-<span class="text-smallcaps">D</span>-galactopyranose, 5-Bromo-4-chloro-3-indolyl-.beta.-D-galactoside, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside, 5-Bromo-4-chloro-3-indoyl-β-galactopyranoside, 5-Bromo-4-chloroindol-3-yl β-<span class="text-smallcaps">D</span>-galactopyranoside, 5-Bromo-4-chloroindol-3-yl β-<span class="text-smallcaps">D</span>-galactoside, Galactopyranoside, 5-bromo-4-chloroindol-3-yl, β-<span class="text-smallcaps">D</span>-, Indole, 5-bromo-4-chloro-3-(β-<span class="text-smallcaps">D</span>-galactopyranosyloxy)-, Xgal, β-<span class="text-smallcaps">D</span>-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl, β-D-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl, Indole, 5-bromo-4-chloro-3-(β-D-galactopyranosyloxy)-, Galactopyranoside, 5-bromo-4-chloroindol-3-yl, β-D-, 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside, 5-Bromo-4-chloroindol-3-yl β-D-galactopyranoside

Descrizione

X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli ) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing beta;-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta;-D-galactopyranoside (X-beta;-D-galactoside, X-Gal) is a chromogenic indicator for beta;-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate.
X-Gal can also be used as a detecting agent for beta;-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta;-galactosidase, color develops within minutes to few hours.

• Marchio: Biosynth

Proprietà chimiche

• Peso molecolare: 408.63 g/mol
• Formula: C₁₄H₁₅BrClNO₆
• Purezza: Min. 98 Area-%
• Colore/Forma: White Powder
• InChI: InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• InChI key: InChIKey=OPIFSICVWOWJMJ-AEOCFKNESA-N
• SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O