Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit
Rif. EK-ELK0650
| 48T | 401,00 € | ||
| 96T | 574,00 € |
Informazioni sul prodotto
- Anti-Insulin; Antiinsulin; Insulin Antibodies
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK0650 Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit
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