Human PUMA(p53 Upregulated Modulator Of Apoptosis) ELISA Kit
Rif. EK-ELK2674
48T | 402,00 € | ||
96T | 575,00 € |
Informazioni sul prodotto
- JFY1; BBC3; BCL2 Binding Component 3
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PUMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PUMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PUMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PUMA in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK2674 Human PUMA(p53 Upregulated Modulator Of Apoptosis) ELISA Kit
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