Mouse IkBKb(Inhibitor Of κ-Light Polypeptide Gene Enhancer In B-Cells Kinase β) ELISA Kit
Rif. EK-ELK6139
48T | 404,00 € | ||
96T | 575,00 € |
Informazioni sul prodotto
- IKK2; NFKBIKB; IKK-beta; IKKB; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBKb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBKb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBKb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBKb in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK6139 Mouse IkBKb(Inhibitor Of κ-Light Polypeptide Gene Enhancer In B-Cells Kinase β) ELISA Kit
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