Rat OGDH(Oxoglutarate Dehydrogenase) ELISA Kit
Rif. EK-ELK7075
| 48T | 402,00 € | ||
| 96T | 576,00 € |
Informazioni sul prodotto
- AKGDH; E1k; OGDC; Alpha-Ketoglutarate Dehydrogenase,Lipoamide; 2-Oxoglutarate Dehydrogenase E1 Component,Mitochondrial
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OGDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OGDH in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK7075 Rat OGDH(Oxoglutarate Dehydrogenase) ELISA Kit
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