Mouse 4-Hydroxynonenal(HNE) ELISA Kit
Rif. EK-ELK8374
| 48T | 404,00 € | ||
| 96T | 574,00 € |
Informazioni sul prodotto
- 4-HNE
- HNE
- 4-hydroxy-2-nonenal
- 6-Hydroxynonenal
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK8374 Mouse 4-Hydroxynonenal(HNE) ELISA Kit
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